ISPa56
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KR258747 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Ps402 |
DNA section
IS Length : 1359 bp
Ends
IR Length : 27/36
IRL : TGAGATAGCCCCCTAATTCCCCGGCCCCGTCCTATCGCTTATCTTTGAAG
IRR : TGATGTTGCCCCCGTATTCCCGACCCCGCCCTATTCGCAATAAAGCGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTCTGCCAT | CACA | GTGCCGCAGT | 4 |
DNA sequence
TGAGATAGCCCCCTAATTCCCCGGCCCCGTCCTATCGCTTATCTTTGAAGATAGGAACAGGACTGTGCATATGACTAGGCATACGGAGTCAGTAGAAGTA
GTCGTGAAGGACCAGCGCCGCAGGCGCTGGTCTCTCGCAGAAAAGGCGGCGCTGGTGCGCCGAACCTACGAACCAGGCATGAGCGTGTCATTAGTCGCTC
GGCAGGAAGGCGTCTCGGCTGGACTGCTGTTCCAGTGGCGCAAGCTCGAACGCCAGGGTGCTTTGACCGCCGTCTCCGCTGGCGAGGCAGTCGTGCCCGC
ATCCGAGCTGGCGGCTGCCCGCGCGGAAATCGCCAAGCTGCAGCGCGTGCTCGGCAAGAAAACCTTGGAGAACGAAATCCTCAAGGAAGCCGTGGAGTAC
GCCGCAGAAAAAAAGTGGATTGCGCGCTCGCCCTTGTTGCCCGGGGACGACCAGTGAAAACGGTCTGCCGGACGATGGGGCTGGCGCGCTCGCATGTGCG
CGACCTGCTTGGGCGCGGTGAAGACTGGACCGATGGCCGCAGCGATCGCACACCCAGCGACGATGCCGGGCTGCTCGCCGAACTGCGCCAGGAAATTGCC
GACTTGCCCAGCTATGGCTACCGACGTGCCTGTGCCTTGGTGAACCGCCAGCGCACGGCACGGGGCGCCCCGAGGGTCAATGCCAAACGCGTCTATCGCG
TCATGGCACAAGCCGCTCTGCTACTGCCCAAGGCGCCTCGCCGGCGCCAGTCAGCACGCACGCATGAGGGCCGGATTGCGGTCTCACGCAGCGACCTGCG
GTGGTGCTCCGATGGTCTGGAAATCAAGTGCGATTCGGGCCAGACCGTGACGGCCACCTTCGCCAAGGACTGCTGCGACCGTGAGGTGATGGCTTGGCGC
GCGTGGGAGGGCAAAGGGCTGCCAGGTGAGCCGGTACGCGAGATGCTCATCGAGGCCGTGGAGCGCCGCTTCGGCTCGGTCGAAGCGGTCCCGCAGGGCC
AGGAGTTGGAGTTCCTCAGTGACAACGGCGGTGCCTACATCGCCGCCGAAACGAGGGCGCTGGCACGCGCTCTGGGCCTGAAGCCCATTAACACTCCCGT
CTGCAGCCCGCAGAGCAACGGCATGGCCGAGAGCTTCGTCAACACCTTCAAGCGCGACTATGTGGCGCGCATGGACCTGAGTGACGCGCAGACGGTACTG
GCGCAACTGGCAGCGGCCTTCGAGCACTTCAACGAGGTGCATCCGCATTCTTCGCTGAAAATGCGTTCGCCTCGGGAGTTCAGGCGGCAACAGGCTGCAG
GGGCTGAGCAGGCGCTTTATTGCGAATAGGGCGGGGTCGGGAATACGGGGGCAACATCA
GTCGTGAAGGACCAGCGCCGCAGGCGCTGGTCTCTCGCAGAAAAGGCGGCGCTGGTGCGCCGAACCTACGAACCAGGCATGAGCGTGTCATTAGTCGCTC
GGCAGGAAGGCGTCTCGGCTGGACTGCTGTTCCAGTGGCGCAAGCTCGAACGCCAGGGTGCTTTGACCGCCGTCTCCGCTGGCGAGGCAGTCGTGCCCGC
ATCCGAGCTGGCGGCTGCCCGCGCGGAAATCGCCAAGCTGCAGCGCGTGCTCGGCAAGAAAACCTTGGAGAACGAAATCCTCAAGGAAGCCGTGGAGTAC
GCCGCAGAAAAAAAGTGGATTGCGCGCTCGCCCTTGTTGCCCGGGGACGACCAGTGAAAACGGTCTGCCGGACGATGGGGCTGGCGCGCTCGCATGTGCG
CGACCTGCTTGGGCGCGGTGAAGACTGGACCGATGGCCGCAGCGATCGCACACCCAGCGACGATGCCGGGCTGCTCGCCGAACTGCGCCAGGAAATTGCC
GACTTGCCCAGCTATGGCTACCGACGTGCCTGTGCCTTGGTGAACCGCCAGCGCACGGCACGGGGCGCCCCGAGGGTCAATGCCAAACGCGTCTATCGCG
TCATGGCACAAGCCGCTCTGCTACTGCCCAAGGCGCCTCGCCGGCGCCAGTCAGCACGCACGCATGAGGGCCGGATTGCGGTCTCACGCAGCGACCTGCG
GTGGTGCTCCGATGGTCTGGAAATCAAGTGCGATTCGGGCCAGACCGTGACGGCCACCTTCGCCAAGGACTGCTGCGACCGTGAGGTGATGGCTTGGCGC
GCGTGGGAGGGCAAAGGGCTGCCAGGTGAGCCGGTACGCGAGATGCTCATCGAGGCCGTGGAGCGCCGCTTCGGCTCGGTCGAAGCGGTCCCGCAGGGCC
AGGAGTTGGAGTTCCTCAGTGACAACGGCGGTGCCTACATCGCCGCCGAAACGAGGGCGCTGGCACGCGCTCTGGGCCTGAAGCCCATTAACACTCCCGT
CTGCAGCCCGCAGAGCAACGGCATGGCCGAGAGCTTCGTCAACACCTTCAAGCGCGACTATGTGGCGCGCATGGACCTGAGTGACGCGCAGACGGTACTG
GCGCAACTGGCAGCGGCCTTCGAGCACTTCAACGAGGTGCATCCGCATTCTTCGCTGAAAATGCGTTCGCCTCGGGAGTTCAGGCGGCAACAGGCTGCAG
GGGCTGAGCAGGCGCTTTATTGCGAATAGGGCGGGGTCGGGAATACGGGGGCAACATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 387 bp | 128 aa | 71 | 457 | + | No |
Description : First part of the transposase
ORF sequence :
MTRHTESVEVVVKDQRRRRWSLAEKAALVRRTYEPGMSVSLVARQEGVSAGLLFQWRKLERQGALTAVSAGEAVVPASELAAARAEIAKLQRVLGKKTLE
NEILKEAVEYAAEKKWIARSPLLPGDDQ
NEILKEAVEYAAEKKWIARSPLLPGDDQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1251 bp | 416 aa | 79 | 1329 | + | No |
Description : Second part of the transposase
ORF sequence :
AYGVSRSSREGPAPQALVSRRKGGAGAPNLRTRHERVISRSAGRRLGWTAVPVAQARTPGCFDRRLRWRGSRARIRAGGCPRGNRQAAARARQENLGERN
PQGSRGVRRRKKVDCALALVARGRPVKTVCRTMGLARSHVRDLLGRGEDWTDGRSDRTPSDDAGLLAELRQEIADLPSYGYRRACALVNRQRTARGAPRV
NAKRVYRVMAQAALLLPKAPRRRQSARTHEGRIAVSRSDLRWCSDGLEIKCDSGQTVTATFAKDCCDREVMAWRAWEGKGLPGEPVREMLIEAVERRFGS
VEAVPQGQELEFLSDNGGAYIAAETRALARALGLKPINTPVCSPQSNGMAESFVNTFKRDYVARMDLSDAQTVLAQLAAAFEHFNEVHPHSSLKMRSPRE
FRRQQAAGAEQALYCE
PQGSRGVRRRKKVDCALALVARGRPVKTVCRTMGLARSHVRDLLGRGEDWTDGRSDRTPSDDAGLLAELRQEIADLPSYGYRRACALVNRQRTARGAPRV
NAKRVYRVMAQAALLLPKAPRRRQSARTHEGRIAVSRSDLRWCSDGLEIKCDSGQTVTATFAKDCCDREVMAWRAWEGKGLPGEPVREMLIEAVERRFGS
VEAVPQGQELEFLSDNGGAYIAAETRALARALGLKPINTPVCSPQSNGMAESFVNTFKRDYVARMDLSDAQTVLAQLAAAFEHFNEVHPHSSLKMRSPRE
FRRQQAAGAEQALYCE
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1259 bp | 417 aa | 71 | 1329 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRHTESVEVVVKDQRRRRWSLAEKAALVRRTYEPGMSVSLVARQEGVSAGLLFQWRKLERQGALTAVSAGEAVVPASELAAARAEIAKLQRVLGTLENE
ILKEAVEYAAEKKVDCALALVARGRPVKTVCRTMGLARSHVRDLLGRGEDWTDGRSDRTPSDDAGLLAELRQEIADLPSYGYRRACALVNRQRTARGAPR
VNAKRVYRVMAQAALLLPKAPRRRQSARTHEGRIAVSRSDLRWCSDGLEIKCDSGQTVTATFAKDCCDREVMAWRAWEGKGLPGEPVREMLIEAVERRFG
SVEAVPQGQELEFLSDNGGAYIAAETRALARALGLKPINTPVCSPQSNGMAESFVNTFKRDYVARMDLSDAQTVLAQLAAAFEHFNEVHPHSSLKMRSPR
EFRRQQAAGAEQALYCE
ILKEAVEYAAEKKVDCALALVARGRPVKTVCRTMGLARSHVRDLLGRGEDWTDGRSDRTPSDDAGLLAELRQEIADLPSYGYRRACALVNRQRTARGAPR
VNAKRVYRVMAQAALLLPKAPRRRQSARTHEGRIAVSRSDLRWCSDGLEIKCDSGQTVTATFAKDCCDREVMAWRAWEGKGLPGEPVREMLIEAVERRFG
SVEAVPQGQELEFLSDNGGAYIAAETRALARALGLKPINTPVCSPQSNGMAESFVNTFKRDYVARMDLSDAQTVLAQLAAAFEHFNEVHPHSSLKMRSPR
EFRRQQAAGAEQALYCE
Blast result :
Comments
ISPa56 is 72% aa similar to ISRso10.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Beatriz Rojo Bezares (2015) Direct submission.