ISHwa30
- Family IS1595
- Group ISH4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FR746099 | ND | Haloquadratum walsbyi | Haloquadratum walsbyi C23 |
DNA section
IS Length : 945 bp
Ends
IR Length : 19/25
IRL : CGCTCTTCTCGATAAGCCGTAACATCAATCTTGCCGGCGGATCATTGATC
IRR : CGCTCATCCCGATACATTGTAACATAGGAGTTTCTCGAACCAATCTTCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAATCAGAATT | ATATGCTA | AAACAAATATGC | 8 |
| AGAAGGCACGAA | TGCAGCAC | AACAAATCATCA | 8 |
DNA sequence
CGCTCTTCTCGATAAGCCGTAACATCAATCTTGCCGGCGGATCATTGATCAGTCAACGATGGATCAAGAGTCTGCTCAAGTCTTCCTTCCATCACGAGAA
CGCTGCTTTGAGCGTCTCCGTCTCGCCCGCTTCGGCGAGACGGTGACGTGTGTCCACTGCGAGAGTGATGCGGTAGTCAAGCGAGGAACCACGGGGAAAG
ATGCCCAGCAGTACTGGTGTAAAAACTGTGAGACATACTTCAACGACCTCACAGACACGATTTTCGGCCAACATCGCTTTGAGCTTGAGGAGCTGTTCTA
TATGGTCAAGGAGATGCGATCTGAGACGACTGCTCAGATCGCCCGTGATCTTGACCGTGACTACGAGGCTGTCCTTGATTTCGTTCACGAAGTCCAGGAG
ACAAGCGGTAACATCGGAGAATTCGAGCTTTCGGAGGTGTGCGAGGCCGATGAGATCTACGTGACTGCCGGTGAGAAAGGACGCGAGGACGAGGATTCGA
GTCCGCGCGAGCGCGGACTAAAAAAAAGGGACGCGGTGACTTCGAGGGAGACAAACCACCAGTCCTGACACTCGTCCGTCGGTCTGACGGACGAGTTCGA
TTCATCGTCTGCAAGGATCTACAGGAAGCCGACGAAGAAATCGCAGCCTACGGCGATGGCAGTGTTATTCTCTGTACGGATGGCTACTCGATCTACGAAG
ATATCGAGGAAAAGGAGGGGGTGGACGGCCATCTGACCGTCACCCACTCCGATACCTACGTCATCGGTGACGCTCACACGAATACTTGTGAGAACCGGCA
TAGTTTCCTTCGCCAGTGGCTGGCGAACTTCAGAGGCGTCTCGAAGCATCATCTACAGAAATATCTCAACTTCCTTGCACTGAAGCTCAATTCACCCGAA
GATTGGTTCGAGAAACTCCTATGTTACAATGTATCGGGATGAGCG
CGCTGCTTTGAGCGTCTCCGTCTCGCCCGCTTCGGCGAGACGGTGACGTGTGTCCACTGCGAGAGTGATGCGGTAGTCAAGCGAGGAACCACGGGGAAAG
ATGCCCAGCAGTACTGGTGTAAAAACTGTGAGACATACTTCAACGACCTCACAGACACGATTTTCGGCCAACATCGCTTTGAGCTTGAGGAGCTGTTCTA
TATGGTCAAGGAGATGCGATCTGAGACGACTGCTCAGATCGCCCGTGATCTTGACCGTGACTACGAGGCTGTCCTTGATTTCGTTCACGAAGTCCAGGAG
ACAAGCGGTAACATCGGAGAATTCGAGCTTTCGGAGGTGTGCGAGGCCGATGAGATCTACGTGACTGCCGGTGAGAAAGGACGCGAGGACGAGGATTCGA
GTCCGCGCGAGCGCGGACTAAAAAAAAGGGACGCGGTGACTTCGAGGGAGACAAACCACCAGTCCTGACACTCGTCCGTCGGTCTGACGGACGAGTTCGA
TTCATCGTCTGCAAGGATCTACAGGAAGCCGACGAAGAAATCGCAGCCTACGGCGATGGCAGTGTTATTCTCTGTACGGATGGCTACTCGATCTACGAAG
ATATCGAGGAAAAGGAGGGGGTGGACGGCCATCTGACCGTCACCCACTCCGATACCTACGTCATCGGTGACGCTCACACGAATACTTGTGAGAACCGGCA
TAGTTTCCTTCGCCAGTGGCTGGCGAACTTCAGAGGCGTCTCGAAGCATCATCTACAGAAATATCTCAACTTCCTTGCACTGAAGCTCAATTCACCCGAA
GATTGGTTCGAGAAACTCCTATGTTACAATGTATCGGGATGAGCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 59 | 568 | + | No |
Description : First part of the transposase
ORF sequence :
MDQESAQVFLPSRERCFERLRLARFGETVTCVHCESDAVVKRGTTGKDAQQYWCKNCETYFNDLTDTIFGQHRFELEELFYMVKEMRSETTAQIARDLDR
DYEAVLDFVHEVQETSGNIGEFELSEVCEADEIYVTAGEKGREDEDSSPRERGLKKRDAVTSRETNHQS
DYEAVLDFVHEVQETSGNIGEFELSEVCEADEIYVTAGEKGREDEDSSPRERGLKKRDAVTSRETNHQS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 468 bp | 155 aa | 475 | 942 | + | No |
Description : Second part of the transposase
ORF sequence :
ERTRGRGFESARARTKKKGRGDFEGDKPPVLTLVRRSDGRVRFIVCKDLQEADEEIAAYGDGSVILCTDGYSIYEDIEEKEGVDGHLTVTHSDTYVIGDA
HTNTCENRHSFLRQWLANFRGVSKHHLQKYLNFLALKLNSPEDWFEKLLCYNVSG
HTNTCENRHSFLRQWLANFRGVSKHHLQKYLNFLALKLNSPEDWFEKLLCYNVSG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 884 bp | 294 aa | 59 | 942 | + | Yes |
Chemistry : DDE
ORF sequence :
MDQESAQVFLPSRERCFERLRLARFGETVTCVHCESDAVVKRGTTGKDAQQYWCKNCETYFNDLTDTIFGQHRFELEELFYMVKEMRSETTAQIARDLDR
DYEAVLDFVHEVQETSGNIGEFELSEVCEADEIYVTAGEKGREDEDSSPRERGLKKKGRGDFEGDKPPVLTLVRRSDGRVRFIVCKDLQEADEEIAAYGD
GSVILCTDGYSIYEDIEEKEGVDGHLTVTHSDTYVIGDAHTNTCENRHSFLRQWLANFRGVSKHHLQKYLNFLALKLNSPEDWFEKLLCYNVSG
DYEAVLDFVHEVQETSGNIGEFELSEVCEADEIYVTAGEKGREDEDSSPRERGLKKKGRGDFEGDKPPVLTLVRRSDGRVRFIVCKDLQEADEEIAAYGD
GSVILCTDGYSIYEDIEEKEGVDGHLTVTHSDTYVIGDAHTNTCENRHSFLRQWLANFRGVSKHHLQKYLNFLALKLNSPEDWFEKLLCYNVSG
Blast result :
Comments
ISHwa30 is 93% aa similar to ISHarch6.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Dyall-Smith,M.L., Pfeiffer,F., Klee,K., Palm,P., Gross,K., Schuster,S.C., Rampp, M., and Oesterhelt,D. (2011) PLoS One 6: e20968
2] Dyall-Smith,M.L., Pfeiffer,F., Klee,K., Palm,P., Gross,K., Schuster,S.C., Rampp, M., and Oesterhelt,D. (2011) PLoS One 6: e20968