ISfinder Mirror Site

ISHti5

Family IS1595
Group ISH4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HF571520	ND	Halorhabdus tiamatea	Halorhabdus tiamatea SARL4B

DNA section

IS Length : 944 bp

Ends

IR Length : 23/27

IRL : CGCTCATCTGTAGCAGTGAATACTTTGTGAGTGGAGTTCGTAGCTGACTC
IRR : CGCTCATCTGTAGAAGTCAGTACTTAGGATTTTCTCGAACCAGCGATCTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTCAATATA	TAGTCAGAT	ACCATATTCG	9
TTGCAGCTGT		TTCTTTGATA	0

DNA sequence

CGCTCATCTGTAGCAGTGAATACTTTGTGAGTGGAGTTCGTAGCTGACTCCAAGATGGTAAGCAGAGAGAGCGTGGATGAGGTCTTTCTGCCTGACAAAG
ATGATTGTCTAGAGTATCTCCGTGAACAGCGATGGCCAGAAGAGGTAATGTGTCCGCACTGCGAGAGTGCGGACACGATCAAGAAGGGGACGACGAGAAA
GGGTGCTCAACGCTATCGCTGTCACAACTGTGATAGTATTTTCAACGATCTTACCGAGACCATATTTGCCGAGCACAAGCTTTCACTCCCGGAGATGTTC
CATATCATTCGAGGGATGGAAGAGGACAAAACATCACAGATAACTCAGGAACTTGACCGAACATACAAGACGGTCTTGGACTTCGTCCATGAAGTCCAAG
ACGCTCTTGACGACGATCCAGAGTTTGATCTCACTGGTGTTTGCGAAGCTGACGAGGTCTACGTCGTGGCTGGTGAGAAAGGTACCAAGCAGGCGAGTCC
GCGCTCGCGCGGACTCAAAAAAAGGGACGCGGAACGTTCGAGTCAGACAAACCGCCAGTCGTGACACTCGTCCGTCGCTCCGACGGACGAGTTCGGTTCC
TCGTTCGTGAAGATCTCGAAGATGTAGACGAGGACATCGTCGAATACGGCGATGAAGACGATCCGGCGATCCTCTGCACCGATCAGTACAGCATCTACGA
CGGTATCGACGAGTACGACGAGATTGATGGCCATCTCGCCATCAATCACGACGAACACTACGTCGTCGGTGATGCTCACACGAACAGCTGTGAGAACCGC
CATAGCTTCCTTCGCAACTGGTTGCGAAGGTTCCGAGGCGTCTCAAAACACCACTTACAGGGCTATTTGAACTTCTTCAGTCTCACACTCAACACAGATC
GCTGGTTCGAGAAAATCCTAAGTACTGACTTCTACAGATGAGCG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
510 bp	169 aa	55	564	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVSRESVDEVFLPDKDDCLEYLREQRWPEEVMCPHCESADTIKKGTTRKGAQRYRCHNCDSIFNDLTETIFAEHKLSLPEMFHIIRGMEEDKTSQITQEL
DRTYKTVLDFVHEVQDALDDDPEFDLTGVCEADEVYVVAGEKGTKQASPRSRGLKKRDAERSSQTNRQS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
465 bp	154 aa	477	941	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ERYQAGESALARTQKKGRGTFESDKPPVVTLVRRSDGRVRFLVREDLEDVDEDIVEYGDEDDPAILCTDQYSIYDGIDEYDEIDGHLAINHDEHYVVGDA
HTNSCENRHSFLRNWLRRFRGVSKHHLQGYLNFFSLTLNTDRWFEKILSTDFYR

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
884 bp	295 aa	55	938	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVSRESVDEVFLPDKDDCLEYLREQRWPEEVMCPHCESADTIKKGTTRKGAQRYRCHNCDSIFNDLTETIFAEHKLSLPEMFHIIRGMEEDKTSQITQEL
DRTYKTVLDFVHEVQDALDDDPEFDLTGVCEADEVYVVAGEKGTKQASPRSRGLKKKGRGTFESDKPPVVTLVRRSDGRVRFLVREDLEDVDEDIVEYGD
EDDPAILCTDQYSIYDGIDEYDEIDGHLAINHDEHYVVGDAHTNSCENRHSFLRNWLRRFRGVSKHHLQGYLNFFSLTLNTDRWFEKILSTDFYR

Blast result :

Comments

ISHti5 is 74% aa similar to ISNpe28.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Pfeiffer F. (2015) Direct submission
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol. In press