ISNpe28

  • Family IS1595
  • Group ISH4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003373 ND Natrinema pellirubrum
Natrinema pellirubrum DSM 15624 plasmid pNATPE01
DNA section
IS Length : 944 bp

Ends


IR Length : 21/25

IRL : CGCTCATCTCGATAAGTCGTAACATCAATTTTCCTTACTCTCCAAGTGTC
IRR : CGCTCATCCCGATACATTGTAACATAGGAGTTTCTCGAACCAGCTATCTG

Insertion site


Left flankDirect repeatRight flankDR Length
ATTGGCCGGTCTAAGGAAAAGAAAGTGT8

DNA sequence

CGCTCATCTCGATAAGTCGTAACATCAATTTTCCTTACTCTCCAAGTGTCTTCCAAGATGGACGAGAAGTCTGCGCAAGTGTTCCTTCCACCACGTGAGC
GGTGCTTTGAACGTCTCCGTCTCGCCCGCTTCGGCGAGACGGTGACGTGTGTCCATTGCGAGAGTGACGACGTTGTCAAGCGCGGAACAACCGGAAAAGA
CGCCCAGCAGTACTGGTGCAAGGGGTGTGAGACGTACTTCAACGACCTCACAAACACGATCTTCGGCCAACATCGCTTCGAACTGGAAGAGATGTTCTAT
ATCGTCAAGGAGATGCGATCTGAGCCGACCGCTCAGATCGCTCGTGACCTTGGCCGAGACTACGAAGCTGTTCTCAACTTCGTTCACAAAGTCCAGGATG
TGAGCGGGGAGATCGATGAATTTGAGCTGTACGATGTCTGTGAAGCTGACGAGATCTACGTAACAGCTGGTGAGAAAGGGATCGAAGACGAGGATCAGAG
TCCGCGCAAGCGCGGACTCAAAAAAAGGGACGAGGACGATTCGAATCAGACAAACCACCGGTGTTGACACTCGTCCGTCGGGATGACGGACGAGTTCGGT
TTCTCGTCTGCAAGGATCTCCAAGACGCCGACGAAGACATCGCTGAATACGGTGATGGAAGCGTCATCCTCTGTACCGATGATTACACAATCTACGACGA
GATCGAGGAGAAGGAGGGGGTGGACGGCCATCTGGCCGTCACCCACTCCGACACCTACGTCATCGGCGATGCCCACACGAACACCTGTGAGAACCGCCAC
AGCTTCCTTCGCCAGTGGCTGGCGAAGTTCAGAGGTGTCTCAAAACATCATCTCCAACGGTATCTCAATTTCCTTGAACTGAAACTCAACGAACCAGATA
GCTGGTTCGAGAAACTCCTATGTTACAATGTATCGGGATGAGCG
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
510 bp169 aa58567+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDEKSAQVFLPPRERCFERLRLARFGETVTCVHCESDDVVKRGTTGKDAQQYWCKGCETYFNDLTNTIFGQHRFELEEMFYIVKEMRSEPTAQIARDLGR
DYEAVLNFVHKVQDVSGEIDEFELYDVCEADEIYVTAGEKGIEDEDQSPRKRGLKKRDEDDSNQTNHRC

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
468 bp155 aa474941+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ERDRRRGSESAQARTQKKGRGRFESDKPPVLTLVRRDDGRVRFLVCKDLQDADEDIAEYGDGSVILCTDDYTIYDEIEEKEGVDGHLAVTHSDTYVIGDA
HTNTCENRHSFLRQWLAKFRGVSKHHLQRYLNFLELKLNEPDSWFEKLLCYNVSG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
881 bp294 aa58938+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDEKSAQVFLPPRERCFERLRLARFGETVTCVHCESDDVVKRGTTGKDAQQYWCKGCETYFNDLTNTIFGQHRFELEEMFYIVKEMRSEPTAQIARDLGR
DYEAVLNFVHKVQDVSGEIDEFELYDVCEADEIYVTAGEKGIEDEDQSPRKRGLKKKGRGRFESDKPPVLTLVRRDDGRVRFLVCKDLQDADEDIAEYGD
GSVILCTDDYTIYDEIEEKEGVDGHLAVTHSDTYVIGDAHTNTCENRHSFLRQWLAKFRGVSKHHLQRYLNFLELKLNEPDSWFEKLLCYNVSG

 

Blast result :
Comments
ISNpe28 is 52% aa similar to ISHvo5.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission to GenBanK.