ISHma14

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY596292 ND Haloarcula marismortui
Haloarcula marismortui ATCC 43049 plasmid pNG300
DNA section
IS Length : 1705 bp

Ends


Left end : CTTGATTCAGCGAGAGAATCCCGCCCTTCCCGTGAGTGACGAGCGTTCCGACGCTTAGTCGGAACCGAGGAGCGAACAGGGCGGGAGTGAATCGCGTAAG II struct. : Yes
Right end : GAGTACCGTCCACCGTGACGGGAATGTTGCTTCCGGGGAATCAGCATAGCCGAGACTCCTACAGAGGAAACCGCGCCGTTCACGGCGCGGAGGATGTCAT II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GTCGCGGCAGTACTATTGATTGGTCGTCtcat

DNA sequence

CTTGATTCAGCGAGAGAATCCCGCCCTTCCCGTGAGTGACGAGCGTTCCGACGCTTAGTCGGAACCGAGGAGCGAACAGGGCGGGAGTGAATCGCGTAAG
CTCCGGTAAGAAACCGCCATGTCACTGCTGGTCTTGAGTCTCCAACACTTCCAGACCAGCTTCTAACACTTCTGCATAGGCTTCCGAGAGGTTCAGGTCG
TTCGCTTCTGCGTAGTCTTTGATTCGTCCGTGGATTGCCCAGTCAATGTCGATGTTTGGCCTCATCGGTGGACTCATTAGACATTGGGACTGATTATACT
TTGGATGAAGCGCACCAACACGTTCGCCGTGCGACCGCTCACCGACGATGGTGAGCAGGTGCTACGGGATCTGTTGGACGCTTCTGCCGCTCTCTGGAAC
GAGATTAACTACCAGCGCCTCATGCGCTACAACGACGAGAACGGCTTTGAGGGCGAAGACGTGTGGGACGCCGATACCGGCGCTCTTGAAGGCAAATACA
AAGGCGTTCTCGGCGCGTCTACCGCTCAAACTGTCCGGCGAGCAAACACCGAAGCGTGGCGGTCGTTCTTCGAGAACAAGAAGGCGTATCACGACAAATC
GAACACGTCGGTCACGGAACACCCGGAACCGCCGGGCTTCCGTGGCAACGAAGATGACGGACGTGTTCTCAAAGGCGTCGTCCGAAAGGACGCCTACACC
GTTGAATGGGGCGACCGCTCTCGACTTGAGATTATCGTCGGCAAAGAACTCCGAGACAGGCACAACAGCCCGAAAAGCCGTCTTCGGCTCGAAATCGTTG
GCGACCCGAACTGGCCTGACTACGAGGACCAAGGCCGGTTGGAACTGTGGTACGACGAGACTGACAGCACCTTCCGAGCTTCGCAACCCGTCACTGTTTC
TGACGATGCACGGGGGACTCCACTGGCCGACCACAAGGCCGCTCTGGACATTGGTGCGAACAATCTCGTCGCCTGTACCACGACGACCGGCAAACAATAC
CTGTACGAAGGCCGCGAGTTGTTTCAGCGATTCCGTGAGACGACACGAGAAATCGCCCGGTTACAGTCCAAGCTACGGGAAGGCCGATACAGTAGCGAGC
GTATTCGGCGGTTGTACCGAAAGCGAACCCGTCGCCGCGACCACGCACAAGAGGCACTGTGTCGTGACCTGCTCGAACGACTCTACGAGGACGGTGTGGA
CACGGTGTATATCGGAGGCTTGGCCGACGTGCTGGACACACACTGGTCGGTCGAGACGAACGCCAAGACGCACAACTTCTGGGCGTTCAAGCAGTTCACC
GAGCGACTGGCGTGTACCGCAGACGAATACGGTATCTCGGTCGAAGTCCGGTCGGAAGCGTGGACCAGTCAAGAGTGCCCACAGTGTGGTTCGACAGACC
GGACGACACGGCATCAGGACACGCTCACCTGTCCGTGTGGATTCGAGGGGCACGCCGACCTTACGGCGTCAGAAACGTTCCTGAAGCGGCATACGAGCAA
AGAAGTCAGGCCGATGGCACGGCCCGTGCGGTTCGAGTGGGACGACCACGAATGGTCGGAGTCACCACGCTCTCTCGAAAGTCCCAAAGAACAGCGCACA
GACCCGAGTACCGTCCACCGTGACGGGAATGTTGCTTCCGGGGAATCAGCATAGCCGAGACTCCTACAGAGGAAACCGCGCCGTTCACGGCGCGGAGGAT
GTCAT
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
144 bp47 aa265122-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRPNIDIDWAIHGRIKDYAEANDLNLSEAYAEVLEAGLEVLETQDQQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1350 bp449 aa3051654+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRTNTFAVRPLTDDGEQVLRDLLDASAALWNEINYQRLMRYNDENGFEGEDVWDADTGALEGKYKGVLGASTAQTVRRANTEAWRSFFENKKAYHDKSN
TSVTEHPEPPGFRGNEDDGRVLKGVVRKDAYTVEWGDRSRLEIIVGKELRDRHNSPKSRLRLEIVGDPNWPDYEDQGRLELWYDETDSTFRASQPVTVSD
DARGTPLADHKAALDIGANNLVACTTTTGKQYLYEGRELFQRFRETTREIARLQSKLREGRYSSERIRRLYRKRTRRRDHAQEALCRDLLERLYEDGVDT
VYIGGLADVLDTHWSVETNAKTHNFWAFKQFTERLACTADEYGISVEVRSEAWTSQECPQCGSTDRTTRHQDTLTCPCGFEGHADLTASETFLKRHTSKE
VRPMARPVRFEWDDHEWSESPRSLESPKEQRTDPSTVHRDGNVASGESA

 

Blast result :
Comments
ISHma14 is 91% aa (TnpB) similar to ISNamo22. The first ORF is a passenger gene annotated as hypothetical protein.
References
1] F. Pfeiffer (2013) Direct submission
2] Baliga,N.S., Bonneau,R., Facciotti,M.T., Pan,M., Glusman,G., Deutsch,E.W., Shannon,P., Chiu,Y., Weng,R.S., Gan,R.R., Hung,P., Date,S.V., Marcotte,E., Hood,L. and Ng,W.V. (2004) Genome Res. 14 (11), 2221-2234