ISBf13
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ417513 | ND | Bacteroides fragilis | Bacteroides uniformis WH207 Bacteroides fragilis 42 plasmid pBFU42e |
DNA section
IS Length : 1220 bp
Ends
IR Length : 14/17
IRL : TTTAGTAGATGACAAAATATAAATTATGTTGTTATATGCCTAATTATTAG
IRR : CTAAGTACATGACAAAAATTTGAATACATCAAATTTCGGGACATAAATTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTTCCACT | ATGTCCTCT | ATATAGTGCA | 9 |
DNA sequence
TTTAGTAGATGACAAAATATAAATTATGTTGTTATATGCCTAATTATTAGGTGTTTATATTTGCAAAAGTCCCTAAGAATCAGTATCTTTAAAGAAAAGT
TTGCCCGCTTTTTCGTATGAAGACAACTGACTTTAAGGACTTGGGCAAAGTTAACCAAATCCTTCCGATTATGCAAGAACATTTTGGGAAATCGATGAAT
CTGGCCCGCATAAAGTTTATGGCTTTCATGCTCCATGCCCTGTGTGTAGTACAGACCGTAAGCCTCCATAAACTCGCGGCGGCAATGCCAACCTCTGTTG
AAAGGGACTCCAACCTTCGCCGCATTCAAAGATTCATAGCCAAGTACGCCCTCAACTTTGACCTTGTGGCGCAGATGATATACTCTCTGCTTCCCGTCAA
GACAGGGCTGGTGCTGAGCATGGACCGTACAAACTGGAAGTTCGGTGATTTCAACATCAACATCCTCATGCTCGGCGTGGCATACAAAGGGATTGCATTC
CCATTGATGTTCAGCCTTCTGCCTAAGAAAGGGAACTCCAATTGGAAGGAACGCAAGGCAATCGTTGAACGATTTGTCCGGCTGTTCGGCTCCGAATGCA
TCGACTCTCTTGTTGCCGACCGGGAGTTTGTCGGCAAGGATTGGATCGGTTGGCTCAACCGCAATCATATACGATATTATATCCGGATCCGGCAGAATTT
CTGGCTTGTCAAGCCTTCCACAGGGGAAAGAATCCGTGCATGGTGGCTCTTCAATTCCCTGAAAGTGGGGCAGGAAAGATTTTATTACAAGCTTTTCCTG
CACAAAGGTGAGTACGTTTATCTTGCCGGAAGCCGAATCAAGAACTCCGACGGTGTGCCTGAACTTCAGATCCTCATCTGCTTCAACCGCCCGGAAAAGG
GTGTCGACACTTATAAAAGGCGATGGGAGATCGAGACCGCATTCCGGGCTATGAAATCTTCCGGATTCAATATCGAAGACACGCATTTGCGCGACACAGA
GCGGATTGCAAGACTTCTGGCAATGGTTTGTATTGCTCTTGTATGGGCGTATCTCGTTGGGGAACATAAAGATGAAAACGTAAAGCCTATAAAGACATTG
AAACATGGACGTAAAGCCAAATCTTTAGTAAAGTACGGCTTGGAGGAAATCTCCAACGTGCTTTTTCGACCAATTTATGTCCCGAAATTTGATGTATTCA
AATTTTTGTCATGTACTTAG
TTGCCCGCTTTTTCGTATGAAGACAACTGACTTTAAGGACTTGGGCAAAGTTAACCAAATCCTTCCGATTATGCAAGAACATTTTGGGAAATCGATGAAT
CTGGCCCGCATAAAGTTTATGGCTTTCATGCTCCATGCCCTGTGTGTAGTACAGACCGTAAGCCTCCATAAACTCGCGGCGGCAATGCCAACCTCTGTTG
AAAGGGACTCCAACCTTCGCCGCATTCAAAGATTCATAGCCAAGTACGCCCTCAACTTTGACCTTGTGGCGCAGATGATATACTCTCTGCTTCCCGTCAA
GACAGGGCTGGTGCTGAGCATGGACCGTACAAACTGGAAGTTCGGTGATTTCAACATCAACATCCTCATGCTCGGCGTGGCATACAAAGGGATTGCATTC
CCATTGATGTTCAGCCTTCTGCCTAAGAAAGGGAACTCCAATTGGAAGGAACGCAAGGCAATCGTTGAACGATTTGTCCGGCTGTTCGGCTCCGAATGCA
TCGACTCTCTTGTTGCCGACCGGGAGTTTGTCGGCAAGGATTGGATCGGTTGGCTCAACCGCAATCATATACGATATTATATCCGGATCCGGCAGAATTT
CTGGCTTGTCAAGCCTTCCACAGGGGAAAGAATCCGTGCATGGTGGCTCTTCAATTCCCTGAAAGTGGGGCAGGAAAGATTTTATTACAAGCTTTTCCTG
CACAAAGGTGAGTACGTTTATCTTGCCGGAAGCCGAATCAAGAACTCCGACGGTGTGCCTGAACTTCAGATCCTCATCTGCTTCAACCGCCCGGAAAAGG
GTGTCGACACTTATAAAAGGCGATGGGAGATCGAGACCGCATTCCGGGCTATGAAATCTTCCGGATTCAATATCGAAGACACGCATTTGCGCGACACAGA
GCGGATTGCAAGACTTCTGGCAATGGTTTGTATTGCTCTTGTATGGGCGTATCTCGTTGGGGAACATAAAGATGAAAACGTAAAGCCTATAAAGACATTG
AAACATGGACGTAAAGCCAAATCTTTAGTAAAGTACGGCTTGGAGGAAATCTCCAACGTGCTTTTTCGACCAATTTATGTCCCGAAATTTGATGTATTCA
AATTTTTGTCATGTACTTAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 367 aa | 195 | 1220 | + | No |
Chemistry : DDE
ORF sequence :
MKTTDFKDLGKVNQILPMMQEHFGKSMNLARIKFMAFMLHALCVVQTVSLHKLAAAMPTSVERDSNLRRIQRFIAKYALNFDLVAQMIYSLLPVKTGLVL
SMDRTNWKFGDFNINILMLGVAYKGIAFPLMFSLLPKKGNSNWKERKAIVERFVRLFGSECIDSLVADREFVGKDWIGWLNRNHIRYYIRIRQNFWLVKP
STGERIRAWWLFNSLKVGQERFYYKLFLHKGEYVYLAGSRIKNSDGVPELQILICFKRPEKGVDTYKRRWEIETAFRAMKSSGFNIEDTHLRDTERIARL
LAMVCIALVWAYLVGEHKDENVKPIKTLKHGRKAKSLVKYGLEEISNVLFRPIYVPKFDVFKFLSCT
SMDRTNWKFGDFNINILMLGVAYKGIAFPLMFSLLPKKGNSNWKERKAIVERFVRLFGSECIDSLVADREFVGKDWIGWLNRNHIRYYIRIRQNFWLVKP
STGERIRAWWLFNSLKVGQERFYYKLFLHKGEYVYLAGSRIKNSDGVPELQILICFKRPEKGVDTYKRRWEIETAFRAMKSSGFNIEDTHLRDTERIARL
LAMVCIALVWAYLVGEHKDENVKPIKTLKHGRKAKSLVKYGLEEISNVLFRPIYVPKFDVFKFLSCT
Blast result :
Comments
ISBf13 is 57% aa similar to ISLpn9.
The sequence of the original KJ417513 file was extended with the data found in AY345595. There are direct repeat of 9 bp and not of 3 bp.
The sequence of the original KJ417513 file was extended with the data found in AY345595. There are direct repeat of 9 bp and not of 3 bp.
References
1] ISfinder submission (2015)
2] Soki,J., Sydenham,T., Fenyvesi,V.S. and Justesen,U (2014) Direct submission GenBank.
3] Gupta,A., Vlamakis,H., Shoemaker,N. and Salyers,A.A. (2003) Appl. Environ. Microbiol. 69 (11), 6455-6463
4] Schlesinger,D.J., Shoemaker,N.B. and Salyers,A.A.(2007)Appl. Environ. Microbiol. 73 (13), 4226-4233.
2] Soki,J., Sydenham,T., Fenyvesi,V.S. and Justesen,U (2014) Direct submission GenBank.
3] Gupta,A., Vlamakis,H., Shoemaker,N. and Salyers,A.A. (2003) Appl. Environ. Microbiol. 69 (11), 6455-6463
4] Schlesinger,D.J., Shoemaker,N.B. and Salyers,A.A.(2007)Appl. Environ. Microbiol. 73 (13), 4226-4233.