ISfinder Mirror Site

ISHla19

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001365	ND	Halorubrum lacusprofundi	Halorubrum lacusprofundi ATCC 49239

DNA section

IS Length : 1851 bp

Ends

Left end : TCTTTTTTCAAGGCGAGAATCCCGCCGTTTACGGCGGGCGTGAAGCCGACAACTCCCTCACGTTCCACCGTCGGTTGCAGTCTGAATACCCCACGACGGC II struct. : Yes
Right end : GCTCACGGCCTACGAAGAGTCGAAACCCTCTACCAGCGACGACTGACTGGTATTCCCCCTGCGTGGGAAGCCTCGCCGTTTACGGCGAGGAGGATGTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GCTCGTCGTCC	TCTA	GTGATGGCGGTC	TCAC
CGCTGTCGGGC	TCTA	ATCCTCGCCGAG	TCAc

DNA sequence

TCTTTTTTCAAGGCGAGAATCCCGCCGTTTACGGCGGGCGTGAAGCCGACAACTCCCTCACGTTCCACCGTCGGTTGCAGTCTGAATACCCCACGACGGC
AAACCTTTATTAGAATTGGGTGTGTAGAAATCAGTACGACCAAATGAAGTACAACCTCGAAACTGGGTCGCACACGGTCTACGCGCTCCAATATCACTTC
GTGACCGTCACGAAGTACCGCGCAGACCTACTCACCGACGAAATCGCAGAACGGGTCGGTGAGATTGCCAGCGACATCTCCGAGGACTTCGGCGTAAACA
TCCAGAACGTCAACGGCGGAAGCGACCACGTTCACATCCTCTTCACGGCGAAGCCAACGACCGACCTCACCAAGTTCATCAACTCACTCAAGGGCGTCAC
ATCTCGCAAAATCCGTGACGAGAACCCCGAGGTTCGGCAGGCGCTCGACAAAGCGTTCTGGCAACCGGGATACTTCCTCACCACCACTGGCCAAGTGAGC
ATCGACGTGCTGATGGAGTACGTGGAGGAGCAGTAGCGTGTCCACGACCGTCACGAAGACGTTGCAGGCGACGTTCGCACCCCCCACCGCGCACAAGCAG
TCGAAACTCAACGACCTGCTCGAAACCTACCGTGACGGTCTGCAAGAAGCGTTCGACGCCGGGGCGAGTACCATGTCCTCGGTGAGCGACATCGTGACGC
CCTACGACTTGCCGTATCAGGCCAAAGCCGCGCTCTGCAACTACGTCCCGAAACTCCGCAAGACGTACAACGCGCAGGATTTGGACGACGAACACCCGAT
ACGACTCACGAACCAAGCCGCGAAGTTCGACCACTCGGAAGAACGCGACTACGAGTTTACGTGGTGGGTTCCTCGTCCCGGTCGGGGAACGAATTTCTGG
ATGCCGCTCCGCATCAATCCCGAACAGGAAGACCTCTGGCACGACCTCGTATCCGAGGACGCGAAAGCGGGCGAGATACGGCTTCAACAGCATCGGAAGA
ATTGGGTACTACACGTCACCGTCGAGTACCCGGTTGAAGAACCAGCGGCGGACGGTGACGCCACGCACATCGGCTTAGACATCGGAGAAACCGCCCTCAT
CACGGGCTGTGCCCTCAAGGACGGTTCTCCGACTGACCCGTTCGTGTGTAGCGGAAGCAGAGCGAAGCATCTCCGAAAAGAGATGCACACGACCCTGAAA
CGACTGCAAGAGCGTGACGCTTCGGAGTGGCGTACCGACGAACGGTTCGACCACTACCAGAACGCCCTCACCGACATCGTGGAGAAAGCGTCTCGGGAAG
CCGTCGAGTACGCCAAGCAGTTTGAAAATCCGGTGTTGGTGATGGAGGACTTGACGTACATCCGTGAGCGTCTCGACTACGGGAAGTACATGAACCGTCG
GCTTCACTCGTGGGCGTTCGCCCGACTCCAAGGGCGCATCGAGGACAAGGCGACGGAAGCAGGTATTCCGGTCGAGTACGTGAATCCGGCGTACACCTCG
CAGACGTGCCACTCGTGCCACCGCATCGGTCGGCGGGACTCGCAGGCCGAGTTCCGGTGTCCGAACGATGACTGCCACGTTTCGACGTTTCAGGCCGACA
TCAACGCTTCCGCGAATATCGCACGACGGGTTGACCCGTGGGGAGAGAGCGTCCCGCTTGACAAGGCCGGACGCGATGACTCGCCTCGGGATGGGAGCGG
TTGTGACACCGCCACGACTCACCGTGAGAAGAGCGTACCAGCGCAGATGACGCTCACGGCCTACGAAGAGTCGAAACCCTCTACCAGCGACGACTGACTG
GTATTCCCCCTGCGTGGGAAGCCTCGCCGTTTACGGCGAGGAGGATGTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
393 bp	130 aa	144	536	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKYNLETGSHTVYALQYHFVTVTKYRADLLTDEIAERVGEIASDISEDFGVNIQNVNGGSDHVHILFTAKPTTDLTKFINSLKGVTSRKIRDENPEVRQA
LDKAFWQPGYFLTTTGQVSIDVLMEYVEEQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1260 bp	419 aa	538	1797	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSTTVTKTLQATFAPPTAHKQSKLNDLLETYRDGLQEAFDAGASTMSSVSDIVTPYDLPYQAKAALCNYVPKLRKTYNAQDLDDEHPIRLTNQAAKFDHS
EERDYEFTWWVPRPGRGTNFWMPLRINPEQEDLWHDLVSEDAKAGEIRLQQHRKNWVLHVTVEYPVEEPAADGDATHIGLDIGETALITGCALKDGSPTD
PFVCSGSRAKHLRKEMHTTLKRLQERDASEWRTDERFDHYQNALTDIVEKASREAVEYAKQFENPVLVMEDLTYIRERLDYGKYMNRRLHSWAFARLQGR
IEDKATEAGIPVEYVNPAYTSQTCHSCHRIGRRDSQAEFRCPNDDCHVSTFQADINASANIARRVDPWGESVPLDKAGRDDSPRDGSGCDTATTHREKSV
PAQMTLTAYEESKPSTSDD

Blast result :

Comments

ISHla19 is 99% (TnpA : the transposase) aa similar to ISHhu2 and 97% (TnpB) aa similar to ISHal1.

References

1] F. Pfeiffer (2013) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.