ISHla18
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001365 | ND | Halorubrum lacusprofundi | Halorubrum lacusprofundi ATCC 49239 |
DNA section
IS Length : 1495 bp
Ends
Left end : GGAGAATCGAGGAGAAAGCCTCGCGCTTTAGCGCGGGGATGAATCCGACATTACCCTCCACAGTCCACCATTCGATGGCACGACTGGATATTCCACGCTG II struct. : Yes
Right end : TTCGCACCGCAAGAACAGGTAACGTCGTAAACCACAATATCCCAACTCAGCGGTGCAGTGCCGTGGGAATCCTCGCGCTTCAGCGCGGGGAGGATGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| AACACCCGAGTA | TTAC | GCGTTCGCGAAC | tcaa |
DNA sequence
GGAGAATCGAGGAGAAAGCCTCGCGCTTTAGCGCGGGGATGAATCCGACATTACCCTCCACAGTCCACCATTCGATGGCACGACTGGATATTCCACGCTG
CGCCAATACACACCTTCAAGTAATTTTGTCTACATAGGTTACGTATGGCGAAACAGGTCGTTACCCGCACCTACACTGCTTCCATACGGAACCAGTCTCG
GGTGCAAGACGACCTTGATTCGCTCGGGTTCGCCGCCTCAAAACTCTGGAACGTCGGACGGTGGACGTGCAGTCGGATCTGGGATAAAATCGATCACATT
CCCACCCACAACGAACTCACCACGTACCTCAAAAACCACGAACGCTATGATGACCTGCATTCTCAGTCAAGTCAGCGAGTCCTTCAAGAACTCGCTGAAG
CGTTCAACGGCTGGTACGGCAACCGACAAAACGGAGATACGAAAGCGAACCCGCCCGGCTACCGCAAACACGGCGACGAGCACCCGCGCTCAACAGTCAC
CTTCAAGCAGAAAGGCTTCAAACTCGACACTCAGTACGACCGAGTTCGACTCTCAAAAGGATCGAACCTGAAAGAGTATTGGTCGGACTTCGTACTGTGC
AAGTACCAAACTCGCCCCGATGTTGACCTCTCCACCGTGGAGAACGTCCAACAAGTCAAGATTGTATGGACGGGTGACGAGTGGGAACTACACTTCGTCT
GTAAGGTCGAAATAGACGTGGATGAAGCCCCCGGTGAGAAGACGGTGGGTGTTGATCTCGGTATCAACAACTTCGCCGCACTCGCCTACGAAGACGGTCA
CAGCGAGCTGTACCCGCTTAACTGCTTGAAACAGGACGACTACTACTTCAGCAAGCTGATTGCTCGGTGTGACAACTCGGACTCCGAGCAGGCCACCCGG
CTGAACCAGAAGAAGTCGGCCCGCCGAACCCACTACTTCCACACCCTCTCCAAGCATATCGTCCAGCGGTGTGTTGACGAGGAAATTGGAACTATCGTGG
TGGGCGATCTCTCCGGCATCCGTGAGGATGAGGAGAACGGCGAGTCAAAGAACTGGGGCAAGCACGGTAATCTTGATTTGCACTCGTGGGCGTTCGACCG
GTTCACCGGCCTCCTCGAATACAAAGCCGAGATGGAAGGCATCACGTTCGAGCAAGTGTCTGAGCGGGATACCTCGAAGTCGTGTTCGTGCTGTGGCCGG
AAGCATGAAGCCAACCGTGTTGAACGCGGGCTGTATGTCTGCGATGAGTGCGGCACAGTGGCGAACGCAGATGTGAACGGCGCTGAGAACATTCGGCAGA
AAGTATCTCCGAGTTCACCGAATCTCTCGGTGAATAGGAGTAACGGCTGGTTGGCACAGCCATCGACGTTGTTGTTTGACAAGGAAACTGGTGCGTTCGC
ACCGCAAGAACAGGTAACGTCGTAAACCACAATATCCCAACTCAGCGGTGCAGTGCCGTGGGAATCCTCGCGCTTCAGCGCGGGGAGGATGTCAA
CGCCAATACACACCTTCAAGTAATTTTGTCTACATAGGTTACGTATGGCGAAACAGGTCGTTACCCGCACCTACACTGCTTCCATACGGAACCAGTCTCG
GGTGCAAGACGACCTTGATTCGCTCGGGTTCGCCGCCTCAAAACTCTGGAACGTCGGACGGTGGACGTGCAGTCGGATCTGGGATAAAATCGATCACATT
CCCACCCACAACGAACTCACCACGTACCTCAAAAACCACGAACGCTATGATGACCTGCATTCTCAGTCAAGTCAGCGAGTCCTTCAAGAACTCGCTGAAG
CGTTCAACGGCTGGTACGGCAACCGACAAAACGGAGATACGAAAGCGAACCCGCCCGGCTACCGCAAACACGGCGACGAGCACCCGCGCTCAACAGTCAC
CTTCAAGCAGAAAGGCTTCAAACTCGACACTCAGTACGACCGAGTTCGACTCTCAAAAGGATCGAACCTGAAAGAGTATTGGTCGGACTTCGTACTGTGC
AAGTACCAAACTCGCCCCGATGTTGACCTCTCCACCGTGGAGAACGTCCAACAAGTCAAGATTGTATGGACGGGTGACGAGTGGGAACTACACTTCGTCT
GTAAGGTCGAAATAGACGTGGATGAAGCCCCCGGTGAGAAGACGGTGGGTGTTGATCTCGGTATCAACAACTTCGCCGCACTCGCCTACGAAGACGGTCA
CAGCGAGCTGTACCCGCTTAACTGCTTGAAACAGGACGACTACTACTTCAGCAAGCTGATTGCTCGGTGTGACAACTCGGACTCCGAGCAGGCCACCCGG
CTGAACCAGAAGAAGTCGGCCCGCCGAACCCACTACTTCCACACCCTCTCCAAGCATATCGTCCAGCGGTGTGTTGACGAGGAAATTGGAACTATCGTGG
TGGGCGATCTCTCCGGCATCCGTGAGGATGAGGAGAACGGCGAGTCAAAGAACTGGGGCAAGCACGGTAATCTTGATTTGCACTCGTGGGCGTTCGACCG
GTTCACCGGCCTCCTCGAATACAAAGCCGAGATGGAAGGCATCACGTTCGAGCAAGTGTCTGAGCGGGATACCTCGAAGTCGTGTTCGTGCTGTGGCCGG
AAGCATGAAGCCAACCGTGTTGAACGCGGGCTGTATGTCTGCGATGAGTGCGGCACAGTGGCGAACGCAGATGTGAACGGCGCTGAGAACATTCGGCAGA
AAGTATCTCCGAGTTCACCGAATCTCTCGGTGAATAGGAGTAACGGCTGGTTGGCACAGCCATCGACGTTGTTGTTTGACAAGGAAACTGGTGCGTTCGC
ACCGCAAGAACAGGTAACGTCGTAAACCACAATATCCCAACTCAGCGGTGCAGTGCCGTGGGAATCCTCGCGCTTCAGCGCGGGGAGGATGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1281 bp | 426 aa | 145 | 1425 | + | No |
AG : TnpB
ORF sequence :
MAKQVVTRTYTASIRNQSRVQDDLDSLGFAASKLWNVGRWTCSRIWDKIDHIPTHNELTTYLKNHERYDDLHSQSSQRVLQELAEAFNGWYGNRQNGDTK
ANPPGYRKHGDEHPRSTVTFKQKGFKLDTQYDRVRLSKGSNLKEYWSDFVLCKYQTRPDVDLSTVENVQQVKIVWTGDEWELHFVCKVEIDVDEAPGEKT
VGVDLGINNFAALAYEDGHSELYPLNCLKQDDYYFSKLIARCDNSDSEQATRLNQKKSARRTHYFHTLSKHIVQRCVDEEIGTIVVGDLSGIREDEENGE
SKNWGKHGNLDLHSWAFDRFTGLLEYKAEMEGITFEQVSERDTSKSCSCCGRKHEANRVERGLYVCDECGTVANADVNGAENIRQKVSPSSPNLSVNRSN
GWLAQPSTLLFDKETGAFAPQEQVTS
ANPPGYRKHGDEHPRSTVTFKQKGFKLDTQYDRVRLSKGSNLKEYWSDFVLCKYQTRPDVDLSTVENVQQVKIVWTGDEWELHFVCKVEIDVDEAPGEKT
VGVDLGINNFAALAYEDGHSELYPLNCLKQDDYYFSKLIARCDNSDSEQATRLNQKKSARRTHYFHTLSKHIVQRCVDEEIGTIVVGDLSGIREDEENGE
SKNWGKHGNLDLHSWAFDRFTGLLEYKAEMEGITFEQVSERDTSKSCSCCGRKHEANRVERGLYVCDECGTVANADVNGAENIRQKVSPSSPNLSVNRSN
GWLAQPSTLLFDKETGAFAPQEQVTS
Blast result :
Comments
ISHla18 is 92% (TnpB) aa similar to ISH39.
References
1] F. Pfeiffer (2013) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.