ISfinder Mirror Site

ISHla16

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001365	ND	Halorubrum lacusprofundi	Halorubrum lacusprofundi ATCC 49239

DNA section

IS Length : 1836 bp

Ends

Left end : GGAAAATTCGAGGCGAAAGCCTCGCCCTTCAGGGCGGGGATGAAGCCGACCAACAGTATTCAACCGCCGACGATGGCATAGACGGGGTTTCCAACGCAAT II struct. : Yes
Right end : TCCCAAAGAAGTGCGCACGAACCCGCAAGTTGCCTCCGTGGGTCGGTAGCCGAACCCTCAACGGAGGAATCCTCGCGCTTTAGCGCGGGGAGGATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CCACTCGGTAGG	TTAC	TTACACTCTCGA	TCAA
CGCTGTCGCGAG	TTAC	GCTTGCTTCGTC	TCAA
GCAGTCGTCGCT	TTAC	CGCCACTCCGAC	TCAA

DNA sequence

GGAAAATTCGAGGCGAAAGCCTCGCCCTTCAGGGCGGGGATGAAGCCGACCAACAGTATTCAACCGCCGACGATGGCATAGACGGGGTTTCCAACGCAAT
CATTAACCCGTCCGACCGTGATAGTATGCATAGATGGTGAAGAGTACCCGTCACGCGAAATACGAACTCTACTACCACATAGTGTTCGTGCCGAAATATC
GGCGTTCGCACCTGACGGGGAAGACGAAGGAACGTCTCGAATCCATCTTCGCGGAAATCTGTGAGGACAAGGGCCTCGAACTGGCCGAGTCCGAGGTCAT
GCCCGACCACGTACACCTGTTCATCGGGAGTCCACCGAAGAACGCCCCGTCGCTCATCGTCAACTGGGTGAAGGGCATCTCCGCCCGCAAGTACAACCAG
CGGTACGACGACCGCGTGAAGTGGACTCGTTCGTACTACGTCGGAACAGCGGGAAGCGCCTCGAAGGGCGCTGTCGAACAGTACATCGCTGAACAGGAGG
GTGACGACGAATGAAGCGTGCCAACACTTTTGAGGTCGTGCCACAGACCGAGAACGACAAAGAGTGCCTCCTACGGCTACTCGATGCATCCGCTTCTCTG
TGGAACGAACTGACCTACGAACGTCGTCAGAACTACTTCGGTGACGGCGACGTGTGGGACACTTCCGAGTACCGAGGACGCTACAACGGCGTCGTCGGAA
GCGCGACTGTTCAACAGGTCACGCGCAAGAACAGCGAAGCGTGGCGGTCGTTCTTCGCCCTCAAGGAGAAAGGCGAGTACGCCAACCCACCGTCGTACTG
GGGCAACGAGGAGGACGGACGCGAACTCCGTACCTACATCCGATGCAACCAGTACACGATTGAGTGGGGGAAACGTAGCCGTCTCGAAATCCCTGTCGGG
CAAGAACTGAAAGACGAATACGGACTCGGCTACCACGAACGACTCCGCCTCGAAGTCCGAGGCAACCCGAAGTGGGACGGCAAACAGGGTCGTCTGGAAC
TTGAGTACGACGAGGTTAGCGACACGTTCAGGGCTTTTCAACCAGTCACCGTACCTGATTCTCGACTGGATTCACCACTGGCTTCGGAAGAAGCCGCCCT
CGACGTTGGAGCGAACAATCTCGTCGCGTGTTCCACGACTACTGGGAACCAGTACCTCTACGACGGTCGTGAGTTGTTCGGACGGTTCCGCGAGACGACA
GACGAAATCGCCCGCCTACAGTCGAAACTCCGAGAGGGTCGCTACTCCTCGAATCGGATTCGACGGCTGTACCGACAGCGGACGAAGCGTCGTGACCATG
CACAGAACGCGCTGGTGCGCGACCTCGTTGAACGGCTGTACGATGAGGGCGTGGCGACGGTGTACGTGGGCGACCTGACAGACGTGCTGGAAGCGCATTG
GTCGGTCAGGGTGAACGAGAAGACGCACAACTTCTGGGCGTTCAAGAAGTTCATCCACCGTCTCGCGTGCGTCTGTGAGGAGTACGGCATCAGCCTCGAA
ACCGAGTCGGAAGCGTGGACGAGTCAGACGTGTCCCGAGTGTGGCGACCACGAGAAGACGGTTCGCCACGAGGATACGCTGACGTGTCCATGTGGCTTCG
AGGGGCACGCCGACCTCACGGCGTCAGAGACGTTCCTTCGGGAAAACAGCAATTGCGAAATCAGGCCGATGGCACGGCCCGTGCGATTCGAGTGGGACGA
CCACGACTGGTCGGGGAAACTATACCCTCACGAAAGTCCCAAAGAAGTGCGCACGAACCCGCAAGTTGCCTCCGTGGGTCGGTAGCCGAACCCTCAACGG
AGGAATCCTCGCGCTTTAGCGCGGGGAGGATGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
381 bp	126 aa	134	514	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MVKSTRHAKYELYYHIVFVPKYRRSHLTGKTKERLESIFAEICEDKGLELAESEVMPDHVHLFIGSPPKNAPSLIVNWVKGISARKYNQRYDDRVKWTRS
YYVGTAGSASKGAVEQYIAEQEGDDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1275 bp	424 aa	511	1785	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRANTFEVVPQTENDKECLLRLLDASASLWNELTYERRQNYFGDGDVWDTSEYRGRYNGVVGSATVQQVTRKNSEAWRSFFALKEKGEYANPPSYWGNE
EDGRELRTYIRCNQYTIEWGKRSRLEIPVGQELKDEYGLGYHERLRLEVRGNPKWDGKQGRLELEYDEVSDTFRAFQPVTVPDSRLDSPLASEEAALDVG
ANNLVACSTTTGNQYLYDGRELFGRFRETTDEIARLQSKLREGRYSSNRIRRLYRQRTKRRDHAQNALVRDLVERLYDEGVATVYVGDLTDVLEAHWSVR
VNEKTHNFWAFKKFIHRLACVCEEYGISLETESEAWTSQTCPECGDHEKTVRHEDTLTCPCGFEGHADLTASETFLRENSNCEIRPMARPVRFEWDDHDW
SGKLYPHESPKEVRTNPQVASVGR

Blast result :

Comments

ISHla16 is 97% (TnpA : the transposase) and 97% (TnpB) aa similar to ISHhu1.

References

1] F. Pfeiffer (2013) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.