ISHti2
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HF571521 | ND | Halorhabdus tiamatea | Halorhabdus tiamatea SARL4B |
DNA section
IS Length : 1616 bp
Ends
Left end : GGATGGCAAGTGGAAGCCCACCTATTCACGGGTGGGTCCGAGCGACAGCACGGCCCGACAGTCTACACGCTACGGCTCGTCTGGATGTTCCACATTGTCG II struct. : Yes
Right end : ACAATCGGTGTAGGAGAAAGTCGCTCGTGTGGTGGTTCGGCATGAGCCGAGACCGCCAACGCGAGGAATCCTCATCCCTTCAGGGTGGGGAGGATGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GAACGCCATCAA | TGAC | GGACTTCGTCTT | tcaa |
DNA sequence
GGATGGCAAGTGGAAGCCCACCTATTCACGGGTGGGTCCGAGCGACAGCACGGCCCGACAGTCTACACGCTACGGCTCGTCTGGATGTTCCACATTGTCG
AGTCCGGTTCTCACTAACTCGATGTATGCTTCGGTGAGAGTCTGGTCAGTGTCTTCGGCCCACTCTTTCACTTGGCCGTGGAGCGACCACGGAATATCCA
CGTTCGGACGCATCAGTGACAAGACTTTAGGACCACAAGACCATAGAAGTTGGTGTCGTGAGGCGAACCAACACGTTTGCGGTGCGGCCCCGCTCCGAGC
AGGACGAGCGACTGCTCCGCGACCTGTTGGACGCCTCCGCCAGCCTGTGGAACGAGTTGAACTACGAGCGCCGCCAGAACTTCTTCGACGGCGAATCGGT
GTGGGACACAGCCGACTACCGCAAGCAGTACGTCGGCGTCCTCGGCTCCGCCACCGCTCAACAAGTCATCCGCAAGAACAGCGAGGCGTGGCGGTCGTTC
TTCGCCGCCCACGAGGATGGCGAGGACACTGCCCCACCGGGATACTGGGGCAACGAGGACGAGGGACGGGAGTTGCGAACGTACATCCGCAACGACCAGT
ACACGTTGGAAATGGGCGACCGCTCCCGTCTCGAAATCCCGGTCGGCTCCGACCTCAAGGACGAGTACGATCTCGGGCATACCGAGCGCCTACGCCTCGA
AGTCGCAGGCGCTCCGAAGTGGGATGGCAAGCAGGGCCGGTTAGAACTGTACTACGACGAGTTGAGCGACCAATTCAGAGCCATTCAACCTGTCACGGTA
GACAAATCTCGACAGGACTCATCATTAGCCGACGAGACGGCTGCTCTGGATATCGGCGCAAACAACATCGTCGCCTGTACGACCACGACCGGCCAGCAGT
ATCTGTACGAGGGGCGCGACCTCTTCGAGCGATTCCGCGAGACAACACGAGAAATCGCACGGCTACAGTCGAAGCTCCAGGAGGGGCGGCACACGTCGAA
GCGGATCCAACGCCTGTACCGTCGCCGGACCCGCCGGCGCGACCACGCTATGGACGGGCTCGCCCGCGATCTCATGGATCGACTGTACGCCGAGGGCGTT
TCGAGGGTGTTCGTCGGCGACCTCACCGACGTGCTCGAAACACACTGGTCGGTGCGGACGAACCAGAAGACGCACAACTTCTGGGCGTTCCGCGCGTTCA
TCGACCGACTTGCGTGCACCGCCGAAGAGTTCGGCATCACGGTCGAAGTTCGAACGGAAGCGTGGACGAGTCAGACGTGTCCGAACTGCGGTTCAACCGA
CCGGACGACGCGGCATCAGGACACCCTCACGTGTCCGTGTGGCTTCGAGGGGCACGCCGACCTCACGGCGTCAGAGACGTTCCTCAGGCGGCACGAAACA
GACGTACCACGGCCGATGGCACGGCCCGTGCGATTCGAGTGGGACGACCACCAGTGGTCGGAGTTACCATACTCTCACGAAAGTCCCAAAGAAGTGCGCA
CAGACCAGAGTATCCTACAATCGGTGTAGGAGAAAGTCGCTCGTGTGGTGGTTCGGCATGAGCCGAGACCGCCAACGCGAGGAATCCTCATCCCTTCAGG
GTGGGGAGGATGTCAA
AGTCCGGTTCTCACTAACTCGATGTATGCTTCGGTGAGAGTCTGGTCAGTGTCTTCGGCCCACTCTTTCACTTGGCCGTGGAGCGACCACGGAATATCCA
CGTTCGGACGCATCAGTGACAAGACTTTAGGACCACAAGACCATAGAAGTTGGTGTCGTGAGGCGAACCAACACGTTTGCGGTGCGGCCCCGCTCCGAGC
AGGACGAGCGACTGCTCCGCGACCTGTTGGACGCCTCCGCCAGCCTGTGGAACGAGTTGAACTACGAGCGCCGCCAGAACTTCTTCGACGGCGAATCGGT
GTGGGACACAGCCGACTACCGCAAGCAGTACGTCGGCGTCCTCGGCTCCGCCACCGCTCAACAAGTCATCCGCAAGAACAGCGAGGCGTGGCGGTCGTTC
TTCGCCGCCCACGAGGATGGCGAGGACACTGCCCCACCGGGATACTGGGGCAACGAGGACGAGGGACGGGAGTTGCGAACGTACATCCGCAACGACCAGT
ACACGTTGGAAATGGGCGACCGCTCCCGTCTCGAAATCCCGGTCGGCTCCGACCTCAAGGACGAGTACGATCTCGGGCATACCGAGCGCCTACGCCTCGA
AGTCGCAGGCGCTCCGAAGTGGGATGGCAAGCAGGGCCGGTTAGAACTGTACTACGACGAGTTGAGCGACCAATTCAGAGCCATTCAACCTGTCACGGTA
GACAAATCTCGACAGGACTCATCATTAGCCGACGAGACGGCTGCTCTGGATATCGGCGCAAACAACATCGTCGCCTGTACGACCACGACCGGCCAGCAGT
ATCTGTACGAGGGGCGCGACCTCTTCGAGCGATTCCGCGAGACAACACGAGAAATCGCACGGCTACAGTCGAAGCTCCAGGAGGGGCGGCACACGTCGAA
GCGGATCCAACGCCTGTACCGTCGCCGGACCCGCCGGCGCGACCACGCTATGGACGGGCTCGCCCGCGATCTCATGGATCGACTGTACGCCGAGGGCGTT
TCGAGGGTGTTCGTCGGCGACCTCACCGACGTGCTCGAAACACACTGGTCGGTGCGGACGAACCAGAAGACGCACAACTTCTGGGCGTTCCGCGCGTTCA
TCGACCGACTTGCGTGCACCGCCGAAGAGTTCGGCATCACGGTCGAAGTTCGAACGGAAGCGTGGACGAGTCAGACGTGTCCGAACTGCGGTTCAACCGA
CCGGACGACGCGGCATCAGGACACCCTCACGTGTCCGTGTGGCTTCGAGGGGCACGCCGACCTCACGGCGTCAGAGACGTTCCTCAGGCGGCACGAAACA
GACGTACCACGGCCGATGGCACGGCCCGTGCGATTCGAGTGGGACGACCACCAGTGGTCGGAGTTACCATACTCTCACGAAAGTCCCAAAGAAGTGCGCA
CAGACCAGAGTATCCTACAATCGGTGTAGGAGAAAGTCGCTCGTGTGGTGGTTCGGCATGAGCCGAGACCGCCAACGCGAGGAATCCTCATCCCTTCAGG
GTGGGGAGGATGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1272 bp | 423 aa | 258 | 1529 | + | No |
AG : TnpB
ORF sequence :
MRRTNTFAVRPRSEQDERLLRDLLDASASLWNELNYERRQNFFDGESVWDTADYRKQYVGVLGSATAQQVIRKNSEAWRSFFAAHEDGEDTAPPGYWGNE
DEGRELRTYIRNDQYTLEMGDRSRLEIPVGSDLKDEYDLGHTERLRLEVAGAPKWDGKQGRLELYYDELSDQFRAIQPVTVDKSRQDSSLADETAALDIG
ANNIVACTTTTGQQYLYEGRDLFERFRETTREIARLQSKLQEGRHTSKRIQRLYRRRTRRRDHAMDGLARDLMDRLYAEGVSRVFVGDLTDVLETHWSVR
TNQKTHNFWAFRAFIDRLACTAEEFGITVEVRTEAWTSQTCPNCGSTDRTTRHQDTLTCPCGFEGHADLTASETFLRRHETDVPRPMARPVRFEWDDHQW
SELPYSHESPKEVRTDQSILQSV
DEGRELRTYIRNDQYTLEMGDRSRLEIPVGSDLKDEYDLGHTERLRLEVAGAPKWDGKQGRLELYYDELSDQFRAIQPVTVDKSRQDSSLADETAALDIG
ANNIVACTTTTGQQYLYEGRDLFERFRETTREIARLQSKLQEGRHTSKRIQRLYRRRTRRRDHAMDGLARDLMDRLYAEGVSRVFVGDLTDVLETHWSVR
TNQKTHNFWAFRAFIDRLACTAEEFGITVEVRTEAWTSQTCPNCGSTDRTTRHQDTLTCPCGFEGHADLTASETFLRRHETDVPRPMARPVRFEWDDHQW
SELPYSHESPKEVRTDQSILQSV
Blast result :
Comments
ISHti2 is 84% (TnpB) aa similar to ISNpe11.
References
1] Pfeiffer F. (2015) Direct submission to ISfinder
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol (in press)
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol (in press)