ISfinder Mirror Site

ISHti1

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HF571520	ND	Halorhabdus tiamatea	Halorhabdus tiamatea SARL4B

DNA section

IS Length : 2131 bp

Ends

Left end : CAGGCGGGGCAGGGCGAGTGCCTCGGGGTCGTACGGAAATCAAAGATTTCCGTGATGACGAGACGCCTCCGGCGTCTCGAACCACTTGACCCCGAGGGTG II struct. : No
Right end : GACTAATCCACGGGAAGCCTCGGGGTCGTACGGAAGACAAAGTCTTCCGTGATGACGAGACGCTTCGCGTCTCGAACCACTTGACCCCGAGGCAGTTCAC II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ACGAGATCACGA	TCTA	CTCTGTCGGTGG

DNA sequence

CAGGCGGGGCAGGGCGAGTGCCTCGGGGTCGTACGGAAATCAAAGATTTCCGTGATGACGAGACGCCTCCGGCGTCTCGAACCACTTGACCCCGAGGGTG
AAGGCCGTAAGCCCCGTTAAACGTGTTCCGTTCGCTCGATGTACTGTTCAATCGTATCGGTCGAAACGTCACCAGCCGTCCCGACGTAGTACGATTCCTC
CCAGAACCCACCGCCCCACAGGTATTCCTGTAACAGAGGTTCGTGCTGTTGCCACATCTCTCGTGCTGTAATGCTCTTGACTGTCCGTACAATCTCGCTC
GGGGCGTATTTGGGATGTGCTGACAGAAACAGGTGTACGTGGTCGGGTGAGATGTGCAACGACAGAATCTCGTAGTTGTAGTCGTCGCACACCTCTCGAA
AACTCGATTCCAGTGAATCTTCTATCACACCCAGTATCGGGTGTCGGTACTTCGGACACCACACAAAGTGGTAGTTGACGTTGTATACTGTGTGGTTTGA
CCGTTTCTCGCCCATACAGACAGTACGTGGACAAGACTTAAGCATTTCAGTGAATAAGCAATACGTGTGAGATGACTAAACGACTCACCGTTGACTTCGA
GGACGAGTTATACAAGGAGTTCTCGAAGCAGTGTATCGACGCTGAGAAAACCAAATCCGAAGTCGTGCGTGGACTCGTCAAAGACTGGCTCAACGAATCC
GAAGAATGACCGACTCACAGGCTCTCGTGAAGACGCTGGATTTCCAACTGAACATCCAGAGTGACAACGAGAGCCTGCTGTACGACGCTACACTCGAAGC
ACGGTCGGCGTACAACGAAACCATCCGCCTCGCCAAGCAGGGGGTGGACTGGGACACGATTCCCGACCGCGTGGCCGACGACGCCGACCTCGTGAAGAAC
ACGACACAGCGCGTCGTCGCCAAAGCACTCGGCGCGATGGAGAATTACTACGAGTACGACGACTTCGACCAACCGAGCCACACCAAGGACGGCGCGTACC
CGCTCCGAGCGAACTACGAGGAAGGATACAACCTGTCGCTCACCGACGACGGCGACGTGACGTTCCGCATCAGCGCGAAGCCCTACAATCACGTCAAGGG
CGTACTCGAAGGTGACGACGCCCACCTCGACATTCTCAAGACCGCTCTCACGAGCGACGAGTGGAAGATTGGGACGGCAGAAGCCCTATTCCACAACGAT
AACGCCGAGTTGCACGTCAACGTCACCAACACCGAGCAGACCGTTCGAGACAAACAAGACTCACGGACGGTTGTCGGCGTGGACGTGAACGAGGACAACG
TAGCGTTGACCGCGCTCTCCGAGGATGGCGTCGAGGACACATTGGTCATCGACTTCGCCGAAATCAAGTTCGAGCGTCACCGCTACTTCACGATGCGAAA
GCGCGTGCAGAACGCCGAGAAACCGAGTATGCATGATACGCTTGAAGGACGGGAAGAACGGTTCGTCCGTGACCGACTCCACAAGGTGAGTCGTCACATC
GTGGAGTGGAGCCGTCAGTTCGAGAACCCGTGCATCGTCTTTGAAGACCTCAAAGAGATGCGCGAGAGTATCGACTACGGAACGCGGATGAACCGACGCT
TGCACCACCTGCCGTTCCGTGCGCTTCAATACTACACGTCGTACAAGGCCGCCTTCGAGGGCGTGCCAACGGCGTGGATTGAGCCTGAATACACGAGTCA
GCAGTGTCCGCTGTGTGGACATACAGAACGGGCGAACCGCCGCAAAAAACGGTTCAAGTGCCGGTCGTGCCAGCATCAAGACCACGCCGACCGTGGTGCA
AGCGTCAACATCGCTGTGAAAGGTATCAAGAAGCATCAAGACTGGAATGTGCCTGCTCTCAACAGCCTTCCCCAAGTCAGGAAGGTGTCGAACGAGAGAC
GGAGTCTCTCGTGATCCCGAAAATCTCCGATTTTCGGGCGACGACGGCAGGCATCGGGGGCCGTGGACGCCCCGACCGTGACCTCGGATACCGCGAGAGG
GCATCATCCCGATGGTATCCGAGGCGTGTCCGACTAATCCACGGGAAGCCTCGGGGTCGTACGGAAGACAAAGTCTTCCGTGATGACGAGACGCTTCGCG
TCTCGAACCACTTGACCCCGAGGCAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
399 bp	132 aa	515	117	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEKRSNHTVYNVNYHFVWCPKYRHPILGVIEDSLESSFREVCDDYNYEILSLHISPDHVHLFLSAHPKYAPSEIVRTVKSITAREMWQQHEPLLQEYLW
GGGFWEESYYVGTAGDVSTDTIEQYIERTEHV

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1332 bp	451 aa	706	2037	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTDSQALVKTLDFQLNIQSDNESLLYDATLEARSAYNETIRLAKQGVDWDTIPDRVADDADLVKNTTQRVVAKALGAMENYYEYDDFDQPSHTKDGAYPL
RANYEEGYNLSLTDDGDVTFRISAKPYNHVKGVLEGDDAHLDILKTALTSDEWKIGTAEALFHNDNAELHVNVTNTEQTVRDKQDSRTVVGVDVNEDNVA
LTALSEDGVEDTLVIDFAEIKFERHRYFTMRKRVQNAEKPSMHDTLEGREERFVRDRLHKVSRHIVEWSRQFENPCIVFEDLKEMRESIDYGTRMNRRLH
HLPFRALQYYTSYKAAFEGVPTAWIEPEYTSQQCPLCGHTERANRRKKRFKCRSCQHQDHADRGASVNIAVKGIKKHQDWNVPALNSLPQVRKVSNERRS
LSSNERRSLS*SRKSPIFGRRRQASGAVDAPTVTSDTARGHHPDGIRGVSD

Blast result :

Comments

ISHti1 is 94% (TnpA : the transposase) and 89% (TnpB) aa similar to ISHma7. There is a STOP codon in frame at the end of TnpB.

References

1] Pfeiffer F. (2015) Direct submission to ISfinder
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol (in press)