ISfinder Mirror Site

ISNma17

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001932	ND	Natrialba magadii	Natrialba magadii ATCC 43099

DNA section

IS Length : 1585 bp

Ends

Left end : CTGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAACGAGCGGAGCGAGCGAGTAGGGAGGGGATACAGCGCCGCACGAGTATCAAACACGTTCGATGCCC II struct. : Yes
Right end : GGATACACCTGTATCTGCAAAGCGCGTCGTGGAAACAGGAAGCCCCGCCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GTGAGTTGCTAT	TTAC	TGTCGTCAGCCC	tcac

DNA sequence

CTGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAACGAGCGGAGCGAGCGAGTAGGGAGGGGATACAGCGCCGCACGAGTATCAAACACGTTCGATGCCC
AGCACACAGGCCCACGCAATCGCAACAACTATACGCACAAGTGCGCATAGTGCGCATGTCGTGAGACGGAAGAACATCACCATCCGCGAAGACCAAGCCG
AATGGGTCGAGGAGAACCACCTCAACCTCTCGTCGTTCGTTCGCGGAAAACTCGACGAACTCATCGAAGAACGTTCGTAGATGAACTACAACTACAGGTA
TCGGCTCCGACCGTCCGATGCTCTCGAAGAACAGTTAGCGTGGACTGTCGATACCTGTAGACAGGTCTACAACCACTTCCTCCACCGACTCAACCGAACC
AGCGACACCTCGGCGTACTCCGAGCAGAAACTCCTACCGAGCCTCAAGAAGTGGTGGAGCGAACTGAAACAAGTCCACTCGAAGGTACTACAGAAGGTCG
TACAACGGCTGTACGACAACCTCTCGACGCTTCGCGGTCGGAAAGAGAACGGCTACCGCGTCGGGACGCTCAAGCGGAAGGCTCCGGGCGAGTACCGCAG
TTTCACCTACAGTCAATCCGGCTTCAAGCTCAAGAACACGAGCGGTCGGACGCGGCTGTGGCTCTCGAAACTCGGTGAAATCCCGCTCACCTTCCACCGC
AACCTGCCCGACGACGCCGAAGTCAAGACCGTCACAGTCAAACAGGAACCGACCGGCAAGTGGTACGCTATCCTCGGCGTCGAAACCCCGGACGACCCAC
CGGAGAAGCCCGAGAATCCTGAGCGGTGCGTCGGTATTGACGTAGGGATTCTCAAGTACACCCACGACACCGACGGAACCGCCGTCGAATCCCTCGACCT
GTCCGACGAGCGAGAGCGGTTGGAACGCGCACAGCGCGACCTCTCGCGGAAGGAACACGGCTCGAACAACTGGGAGAAACAGCGCCGTGTCGTGGCCGAA
CGCCACGCCGTGCTGAAGAACAAGCGCCGAGACTTCTTGCACAAACTCTCGAACTACTACGCGAGAGAGTGCGACCTCGTGGCTGTGGAGGACTTAGATG
CGAAGGGATTGGTCGAACTCCCGGGCAACTCTCGAAATCGGGCAGGTGCGGCGTGGGGGACGTTCCTGCGGATGCTCGAATACAAGTGCAAGCAAGAGGG
GACGCACTTCGTGGCAGTCGAACCAGCAGGGACGACCAAGGAGTGTGCGTCCTGCGGCGTTTCGACCGAGAAACCGCTGTGGGTGCGCGAACACTCCTGC
CCTTCCTGCGGGTTCGAGGCAGATAGAGACGCGAACGCGGCGTGGAACATCCTTTCTCGCGGTGTCAAAGAGCGGTTAGGAGCGGGACGCTCCGAATCAA
CGTCCTCAGAATCGCTTTGCGATTCTGATGGGCTGCGAAAATCGCACAGCGATTTTCGAACGCCTGTGGAGACTGCGTTCCCTGTGGATACACCTGTATC
TGCAAAGCGCGTCGTGGAAACAGGAAGCCCCGCCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
186 bp	61 aa	95	280	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPSTQAHAIATTIRTSAHSAHVVRRKNITIREDQAEWVEENHLNLSSFVRGKLDELIEERS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1290 bp	429 aa	281	1570	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEEQLAWTVDTCRQVYNHFLHRLNRTSDTSAYSEQKLLPSLKKWWSELKQVHSKVLQKVVQRLYDNLSTLRGRKENGYRVGTLKRK
APGEYRSFTYSQSGFKLKNTSGRTRLWLSKLGEIPLTFHRNLPDDAEVKTVTVKQEPTGKWYAILGVETPDDPPEKPENPERCVGIDVGILKYTHDTDGT
AVESLDLSDERERLERAQRDLSRKEHGSNNWEKQRRVVAERHAVLKNKRRDFLHKLSNYYARECDLVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRMLE
YKCKQEGTHFVAVEPAGTTKECASCGVSTEKPLWVREHSCPSCGFEADRDANAAWNILSRGVKERLGAGRSESTSSESLCDSDGLRKSHSDFRTPVETAF
PVDTPVSAKRVVETGSPALKERTASAVSE

Blast result :

Comments

ISNma17 is 84% (TnpB) aa similar to ISNagr11. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer, F. (2015) Direct submission
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165