ISNma15
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001932 | ND | Natrialba magadii | Natrialba magadii ATCC 43099 |
DNA section
IS Length : 1475 bp
Ends
Left end : ATAAGAATAGCAGCGCGAGTACCACGGGCCACCGTGCCCGTGGGTGAAGCGCGTCACAACACGAACTTTTAACAAGTGTCAAATTATACTAGAATACAGT II struct. : Yes
Right end : GCAACCAGGGATGGGAGCGCGAGAGGAATTAAAGTCCGTTGCGTCAGCAGTGCAGGACTAAACACGGACGCCCCGCGCCACCGTGCGCGGGGTACTTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| TGATTATGACCG | TCTCT | ACCGGGACCGGG | tcac |
DNA sequence
ATAAGAATAGCAGCGCGAGTACCACGGGCCACCGTGCCCGTGGGTGAAGCGCGTCACAACACGAACTTTTAACAAGTGTCAAATTATACTAGAATACAGT
GCAGTCGTCCAAACTCACCCAATCACTGTCGTTCGGACTAGACATCCACACGGGGAGTGGGGACGACCTGCAAACGGGCTGTATTGAGGCCCGCCGCATC
CGCAACGAAGCCAATCGACTCGACCGAGCCGGTTGGGATTGGGGCGACATTAAATCGACTGTCGTTGATAACGCCGATCACGTCAACAACACCACCCAAC
TCATCGTTGACAAGGCACTCGGTGAAATCGAGACCTATCACGACAACAAAGATGACGGATGGGGTCGACCTTACCCATACATCGACGAGTCGTACCCGAT
GGTGATGAACCACAGCGAAGGCTATCGCCTGTTCCCCGAAGACGACGGGACGGTTCGGTTCCGTATCACTGCCACAAGAGGCACGCACGTCAAAGGCGAA
CTCCGTGGCAACCCCGACCACTTCGACAGACTCCAAATCGCATTCAACGACGAAGAATGGCGGGTTGGAACCGCTGAAGTCGTTCACAAACACAATGAGT
GGCGACTCCACGTAACAGTCACCCACGAGACTCATCGCGTCGCCAGCAAGAACGACGCGGACACAATTGTTGGCGTGGATGTGAACGAGGACTGTATTGC
CCTTGCTGCAATGAGTCGGAACGGTGGGGTGAAACAGTCGGTGGTGTTCGAGTATCCGTCAGTGAAAGAACAGCGACACGAGTTTTTCACCAAGCGCAAG
CGGATACAGAAAGCCGGGCAGACTGCGTTCGAGACGGCCGTGAAGACTGAGGAACGGGACTACGTTCACGATTGCCTACACAAAGTATCGCGGCAGGTAG
TTGACTGGGTCTCGCAATTTACCGACCCGGTGATCGTCTTTGAAGACCTCAAAGACATGCGAGAGTCCATCGACTACGGAACGCGAATGAACCGTCGGCT
ACACTCACTGCCGTTCGCAACTCTCCAAGAGATGGTATCGTACAAAGCTGCGTGGGAAGGGGTTTCTTCAGATGTGGTTGACCCCGAATACACGAGTCAG
CGATGCCCACGGACGGAGTGCCAGCACACAGACCGAGCGAATCGACAGAAGAAGCGATTCAAATGCAAACGGTGTGAGTTCCAAGACCACGCTGATCGGA
AGGCGGCGGTATGCGTTGTGCAGAATTGGTTAGACGAACAGTATGGAAATGTGCTGTCTCTCGAAACCCTTCCACACGTTCGGACGGTGAGACGGGCGGC
ATCGGGGGCTGGTGGAGGCCCCGACTCTCACGGACACGATTTGTGTTCGGGTGTTGACCGACACGGGACGTCGGCGCAACCAGGGATGGGAGCGCGAGAG
GAATTAAAGTCCGTTGCGTCAGCAGTGCAGGACTAAACACGGACGCCCCGCGCCACCGTGCGCGGGGTACTTCAC
GCAGTCGTCCAAACTCACCCAATCACTGTCGTTCGGACTAGACATCCACACGGGGAGTGGGGACGACCTGCAAACGGGCTGTATTGAGGCCCGCCGCATC
CGCAACGAAGCCAATCGACTCGACCGAGCCGGTTGGGATTGGGGCGACATTAAATCGACTGTCGTTGATAACGCCGATCACGTCAACAACACCACCCAAC
TCATCGTTGACAAGGCACTCGGTGAAATCGAGACCTATCACGACAACAAAGATGACGGATGGGGTCGACCTTACCCATACATCGACGAGTCGTACCCGAT
GGTGATGAACCACAGCGAAGGCTATCGCCTGTTCCCCGAAGACGACGGGACGGTTCGGTTCCGTATCACTGCCACAAGAGGCACGCACGTCAAAGGCGAA
CTCCGTGGCAACCCCGACCACTTCGACAGACTCCAAATCGCATTCAACGACGAAGAATGGCGGGTTGGAACCGCTGAAGTCGTTCACAAACACAATGAGT
GGCGACTCCACGTAACAGTCACCCACGAGACTCATCGCGTCGCCAGCAAGAACGACGCGGACACAATTGTTGGCGTGGATGTGAACGAGGACTGTATTGC
CCTTGCTGCAATGAGTCGGAACGGTGGGGTGAAACAGTCGGTGGTGTTCGAGTATCCGTCAGTGAAAGAACAGCGACACGAGTTTTTCACCAAGCGCAAG
CGGATACAGAAAGCCGGGCAGACTGCGTTCGAGACGGCCGTGAAGACTGAGGAACGGGACTACGTTCACGATTGCCTACACAAAGTATCGCGGCAGGTAG
TTGACTGGGTCTCGCAATTTACCGACCCGGTGATCGTCTTTGAAGACCTCAAAGACATGCGAGAGTCCATCGACTACGGAACGCGAATGAACCGTCGGCT
ACACTCACTGCCGTTCGCAACTCTCCAAGAGATGGTATCGTACAAAGCTGCGTGGGAAGGGGTTTCTTCAGATGTGGTTGACCCCGAATACACGAGTCAG
CGATGCCCACGGACGGAGTGCCAGCACACAGACCGAGCGAATCGACAGAAGAAGCGATTCAAATGCAAACGGTGTGAGTTCCAAGACCACGCTGATCGGA
AGGCGGCGGTATGCGTTGTGCAGAATTGGTTAGACGAACAGTATGGAAATGTGCTGTCTCTCGAAACCCTTCCACACGTTCGGACGGTGAGACGGGCGGC
ATCGGGGGCTGGTGGAGGCCCCGACTCTCACGGACACGATTTGTGTTCGGGTGTTGACCGACACGGGACGTCGGCGCAACCAGGGATGGGAGCGCGAGAG
GAATTAAAGTCCGTTGCGTCAGCAGTGCAGGACTAAACACGGACGCCCCGCGCCACCGTGCGCGGGGTACTTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1338 bp | 445 aa | 99 | 1436 | + | No |
AG : TnpB
ORF sequence :
MQSSKLTQSLSFGLDIHTGSGDDLQTGCIEARRIRNEANRLDRAGWDWGDIKSTVVDNADHVNNTTQLIVDKALGEIETYHDNKDDGWGRPYPYIDESYP
MVMNHSEGYRLFPEDDGTVRFRITATRGTHVKGELRGNPDHFDRLQIAFNDEEWRVGTAEVVHKHNEWRLHVTVTHETHRVASKNDADTIVGVDVNEDCI
ALAAMSRNGGVKQSVVFEYPSVKEQRHEFFTKRKRIQKAGQTAFETAVKTEERDYVHDCLHKVSRQVVDWVSQFTDPVIVFEDLKDMRESIDYGTRMNRR
LHSLPFATLQEMVSYKAAWEGVSSDVVDPEYTSQRCPRTECQHTDRANRQKKRFKCKRCEFQDHADRKAAVCVVQNWLDEQYGNVLSLETLPHVRTVRRA
ASGAGGGPDSHGHDLCSGVDRHGTSAQPGMGAREELKSVASAVQD
MVMNHSEGYRLFPEDDGTVRFRITATRGTHVKGELRGNPDHFDRLQIAFNDEEWRVGTAEVVHKHNEWRLHVTVTHETHRVASKNDADTIVGVDVNEDCI
ALAAMSRNGGVKQSVVFEYPSVKEQRHEFFTKRKRIQKAGQTAFETAVKTEERDYVHDCLHKVSRQVVDWVSQFTDPVIVFEDLKDMRESIDYGTRMNRR
LHSLPFATLQEMVSYKAAWEGVSSDVVDPEYTSQRCPRTECQHTDRANRQKKRFKCKRCEFQDHADRKAAVCVVQNWLDEQYGNVLSLETLPHVRTVRRA
ASGAGGGPDSHGHDLCSGVDRHGTSAQPGMGAREELKSVASAVQD
Blast result :
Comments
ISNma15 is 67% (TnpB) aa similar to ISNph7.
References
1] Pfeiffer, F. (2015) Direct submission
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165.
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165.