ISNma12

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001934 ND Natrialba magadii
Natrialba magadii ATCC 43099 pNMAG02
DNA section
IS Length : 1923 bp

Ends


Left end : ACATAGTTCGAGCGCATACCCGCGTCTTCAGGCGCGGGTCGAGCGGTAGGCATAGTTCGTCCGAACTACGTTGCTACGAACCGCTGGATTTCCCGCGTTT II struct. : No
Right end : GACGTGTGGACCGTATGCGTCTTGAAACCAACAGGCCACAACGCTGGGCCTGAAATCCCATGGTGGGATTCCCGCGACTTTAGGCGCGGGAGGAGGTCAA II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
ATCTGATAACTGTGATGAGAGCCGATCG

DNA sequence

ACATAGTTCGAGCGCATACCCGCGTCTTCAGGCGCGGGTCGAGCGGTAGGCATAGTTCGTCCGAACTACGTTGCTACGAACCGCTGGATTTCCCGCGTTT
TCGGTAGTTTTAACAGTAGACTCGATCATAGTGTGTAGTACGGATGAAGACCAAACGGCACGCGACCTACAACCTTAACTATCACATAGTGTGGGTCCCG
AAGTGCCGAAACTCGGTGCTGGTTAACGAGGTCGCCAACCGTGTGCGTCGAATCCTCCACGAGATCGCCGACGAAAAAGGGTTGGAGATCATCGACCTTA
CCGTCCAATCCGACCACGTACACCTATTCGTCAGTAGCCCACCAAAGCACGCTCCCTCACTCCTCGCCAACTGGTTCAAAGGCATTAGTTCCCGGAAGTA
CAACCACCGCCACGCCGACCACGACGGCGAGAAAATCAAGTGGGCACGCGGCTACTATGCCGGAACAGCGGGACACGTCTCAAGCGAGACGGTGCAAGAC
TACATCCAGCGTCAACAGAACTCATGAGTTCTGTTTGGCACACGAGAAACGCGCCGCGTTTCTCGGGACGACACGAGGTGGGAGACACGTGACCGAACTC
ACTGAGACGCTGGAACTCAAACTTGTGGAGCTGAACGCCCACAAGCACCGCAAACTCTGTGAGACAAAACAGGCGTACCAAGACGCCCTCAAAGCCGCGT
TTAACGCGAATTGCACCACCCAGACCGAAGCGAACGATGTGGTGGTCAATTACGACCTGTCGGGATACGCGAAGAACGCCCTCAAGAAGTACGTCCCGCA
ACTCTGTGGGGACAGCTACAACGCTGACGAGCTTCACAACGATCACCCGGTTCGGTTCACCAACGAGGGGCCGAAGCTCGACCACCAGCCACAGAACGCC
CTCGAGTGGTACGTCAAAATCCCACACCACGAAGACTACCATCTCTGGATTCCAGTACAAATCAATCCAAACCAACGGGAGTGGCTCGAGGCGTTATACG
CAGACGACGCTGAAATGGGTGAATGCCGACTCATCGAACGGGACGGGACGTGGTACTTCCACGTCACCGCTACCCGCGACGTGGAGGAACAATCTCAGGC
GTCCGTCGACGAGCGGACGCCGATTGGTGTGGACATCGGAGAAGCGAGTCTCGTCACGGTGTGTCACCGCGACGAGAGCGGTTCTCCGGTTCGCCCCGAA
CTATGGGCCAACGACGGCAAAGCCGTCCGTCGGCTCCGCAAAACCTACTTCACCGCCAAGCGGCGGCTTCAAAAGCGCGGCAGTGAGCGAATCGCGGAGT
CCTACGGTGACACACTTTGGAGCCAGATCGACAATCTATTCCACCGTGTGACCCGCGAGGTCATCGAGTACGCTGAGTCTGTCGAGAATCCAGTATTGGT
ACTGGAAGACCTGACGTACATCCGCGAGAACATGGACTACGGCAAGTATATGAACCGGCGGTTGCACGGGTGGGGCTTTGCGAAGCTCCACGCACAAATC
CGGTACAAAGCCGCCGAGAAAGGGATTCCCGTCGAGACGGTGAATCCCCGGAACACATCAAAAGCGTGCCACGCCTGCGGTGAACATGGCTCCCGGCCGC
GACAGGCGACGTTCCGGTGTTCGAACGACGACTGTTGGCTCGGTGAGTACCAAGCTGACGTGAACGGGGCGATAAACATTGCAGATCGCTACCGTAGTGG
AGAGAGTCACCGCCGAAGTGACCGGGCTTCCCGGCAGAAGGCTGGTGACGATGACTCGGCTACGGATGGGGCCTCTTTGACCGGGCCACAAGACAGCCAC
GCCGATGCTGAAACCCAGCAGAAGACGTGTGGACCGTATGCGTCTTGAAACCAACAGGCCACAACGCTGGGCCTGAAATCCCATGGTGGGATTCCCGCGA
CTTTAGGCGCGGGAGGAGGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa144527+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKTKRHATYNLNYHIVWVPKCRNSVLVNEVANRVRRILHEIADEKGLEIIDLTVQSDHVHLFVSSPPKHAPSLLANWFKGISSRKYNHRHADHDGEKIKW
ARGYYAGTAGHVSSETVQDYIQRQQNS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1260 bp419 aa5891848+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTELTETLELKLVELNAHKHRKLCETKQAYQDALKAAFNANCTTQTEANDVVVNYDLSGYAKNALKKYVPQLCGDSYNADELHNDHPVRFTNEGPKLDHQ
PQNALEWYVKIPHHEDYHLWIPVQINPNQREWLEALYADDAEMGECRLIERDGTWYFHVTATRDVEEQSQASVDERTPIGVDIGEASLVTVCHRDESGSP
VRPELWANDGKAVRRLRKTYFTAKRRLQKRGSERIAESYGDTLWSQIDNLFHRVTREVIEYAESVENPVLVLEDLTYIRENMDYGKYMNRRLHGWGFAKL
HAQIRYKAAEKGIPVETVNPRNTSKACHACGEHGSRPRQATFRCSNDDCWLGEYQADVNGAINIADRYRSGESHRRSDRASRQKAGDDDSATDGASLTGP
QDSHADAETQQKTCGPYAS

 

Blast result :
Comments
ISNma12 is 92% (TnpA) aa similar to ISNamo18 and 86% (TnpB) aa similar to ISNamo17.
References
1] Pfeiffer, F. (2015) Direct submission
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165