ISfinder Mirror Site

ISHarch3

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP002989	ND	Halophilic archaeon	Halophilic archaeon DL31 phalar01

DNA section

IS Length : 1819 bp

Ends

Left end : CCTGAACCAAGGAGAGAGTCCCGCCGTTCACGGCGTTGACGAATCGCTCTGCGATTCGTCCGCACACCAGAAATCGAAGATTTCTGGGGACGTTGACGAG II struct. : Yes
Right end : AGTCGATACCCCCAGCGCGAGGAAACCCCGGCGTCGCCGGGCTCCGCCCGGCTGCCTGAGGGACTTCGTCCCTCTCCGTTCACGGCGAGGAGGATGTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
AAAGAAACCGT	GCTA	AAAAACTGGCCG	tcat

DNA sequence

CCTGAACCAAGGAGAGAGTCCCGCCGTTCACGGCGTTGACGAATCGCTCTGCGATTCGTCCGCACACCAGAAATCGAAGATTTCTGGGGACGTTGACGAG
ACGCAAAGCGTCTCGTTCGCACACCAGAACGCGAAGCGTTCTGGGGACGGGCGTGAATCCGACAACTGCCGAATAACCGCCGACGCCGCTCACTCCGGGG
TATCCAAGCCATCGGCCGCGTGGAGCAACATCCCTTCCCACGTCAGGCCGTGGTTGTCCTTTATTCGCTTCAATCGCTCATGTACGTCGTCGTCCACAAC
TGCGTGGATGTTCTTCGCCATGCATACACACAAAACACAAACATACATTACCCTTCGGATACACCCAGTAGTTGATGAAGCGGACCAACACCTTCGACGT
GATTCCACAGTCCGACGAGGACGAGGAGTTACTCCGACGCCTGTTGGACGCTTCTGCCGCTCTCTGGAACGAAATCAACTACGAGCGCCGCGAACACTAC
GCCGACCCGGACGGAGACGTGTGGGAAATCGGCGAGTATCGCGGACGCTACGGCGGGACGCTCGGCGCTTCGACCGTTCAGCAAATCGAACGCAAGAACC
GCGAAGCGTGGCGGTCGTTCTTCGCGCTCAAGAAGAAGGGCGAAGCCAACGGCAAGCCCGGCTTCTGGGGCAACAAGGACGATGGCCGCGACCTCCGAAC
GTACATCCGCAACACGTCGTATTCGGTCGAGTGGGGCGACTACTCCCGCCTCGAAATCCTCGTCGGGCAAGACCTGAAAGACGAGTACGGGCTTGGACAC
CGCGAACGTCTTCGGCTCGAAGTCCGGGGCGACCCCAACTGGAAAGAGTACGACAAGCAAGGTCGGTTGGAGTTGTTCTACGACGAGCACGCACAGACAT
TCAGGGCCTTTCAGCCAGTCACAATCAGCGAAGACCATTCTCGGCTGGCACACCCACTGGCGGACGAAACCGCCGCACTGGATATTGGTGCGAACAACCT
CGTCGCCTGTACAACCACAACCGGCACGCAACTGCTGTACGAGGGGCGCGATCTGTTCGAGCGATTCCGCGAGACGACGCGCCGAATCGCCGATCAGCAG
TCGAAAGTGAAGCGGGAAGAAGGCCGGTACACGTCGAAACGGATCCGACAGCTGTACCGCCGACGGACGCGGCGGCGCGACCACGCCCAAGACGCACTCG
TCCGCGACCTCGTAGAACGGTTGCATGGAGAAGGCGTCTCCACGGTGTACGTGGGCGCACTCACGGATGTACTCGACACGCACTGGTCGGTCGAAGCGAA
CGCCAAGACGCACAACTTCTGGGCGTTCCGGGCGTTCATCGACCGACTCGCGTGTACTGCCGAAGAGTACGGTATGGCGGTCGAGGTTCGGTCGGAGGCG
TGGACTTCACAAGAGTGTCCAAACTGCGGTTCGGTCGACCGGACGACACGCCACCGCGACACGCTGACGTGCCCGTGCGGTTTCGAGGGGAACGCGGACC
TCACGGCGTCCGAAACGTTCCTAAAGCGTCATCAGAACTCGGAGAGTTCTGATCGGCGAACGAGACACGTCGTGTCTCGTCAACGGCAGACAACGGTATC
ACGGCCGATGGCACGGCCTGTATGCCTCAAGTGGGACGACCACGACTGGTCGGAGTCACCACGCTCTCCCCGTCCCAACGAGGAGCATACGAACCCGCAA
GTTGCCTCCGTGGGTCGGTAGTCGATACCCCCAGCGCGAGGAAACCCCGGCGTCGCCGGGCTCCGCCCGGCTGCCTGAGGGACTTCGTCCCTCTCCGTTC
ACGGCGAGGAGGATGTCAT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
132 bp	43 aa	321	190	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAKNIHAVVDDDVHERLKRIKDNHGLTWEGMLLHAADGLDTPE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1344 bp	448 aa	378	1721	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRTNTFDVIPQSDEDEELLRRLLDASAALWNEINYERREHYADPDGDVWEIGEYRGRYGGTLGASTVQQIERKNREAWRSFFALKKKGEANGKPGFWGN
KDDGRDLRTYIRNTSYSVEWGDYSRLEILVGQDLKDEYGLGHRERLRLEVRGDPNWKEYDKQGRLELFYDEHAQTFRAFQPVTISEDHSRLAHPLADETA
ALDIGANNLVACTTTTGTQLLYEGRDLFERFRETTRRIADQQSKVKREEGRYTSKRIRQLYRRRTRRRDHAQDALVRDLVERLHGEGVSTVYVGALTDVL
DTHWSVEANAKTHNFWAFRAFIDRLACTAEEYGMAVEVRSEAWTSQECPNCGSVDRTTRHRDTLTCPCGFEGNADLTASETFLKRHQNSESSDRRTRHVV
SRQRQTTVSRPMARPVCLKWDDHDWSESPRSPRPNEEHTNPQVASVGR

Blast result :

Comments

ISHarch3 is 77% (TnpB) aa similar to ISHhu1. The first ORF is a passenger gene annotated as hypothetical protein.
There are insertions inside the two extremities of this IS.

References

1] Pfeiffer, F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Dyall-Smith,M., Cavicchioli,R. and Woyke,T. (2011) Direct submission GenBank.