ISfinder Mirror Site

ISHru1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003050	ND	Halovivax ruber	Halovivax ruber XH-70

DNA section

IS Length : 1527 bp

Ends

Left end : GGAAGTGCGAGGAGAAAGCCTCGCCCTTTAGGGCGAGGATGAATCCGACAACGATTGAACAATCCACGTTCGATAGAAAGCCCGAGTCTCCAACGAAAAC II struct. : Yes
Right end : GAGTACCACTGTTCACGGTGGGAATATCGCTCTCGGGGACTCAGACGACTGAGACTCCCACGAGAGGAATCCTCGCGCTTCAGCGCGGGGAGGATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CTTGGACGGAGA	TTAC	GACTCGCCGTCG	tcaa

DNA sequence

GGAAGTGCGAGGAGAAAGCCTCGCCCTTTAGGGCGAGGATGAATCCGACAACGATTGAACAATCCACGTTCGATAGAAAGCCCGAGTCTCCAACGAAAAC
CCATAGTTTTACAAGCACCCAATTGAAATAAATCGAGGATGAAGCGAGCCAACCAGTTCAACGTTCGCCCCCGCTTCACGGAAGAGCGTGAGGTGTTCAA
ACGCTGGTTGGACGCTTCTGCCAGTCTCTGGAACGAGACCAACTACGCTCGCCGCCAAGCCTTCCTCTCAGGCAGCAATGAGAGCGTCTGGGACGCTGAG
ACAGGCGCACTCGGAGGCAAATACAAAGCCGCCCTCAGCAGCTCCGTCGCCCAGCAAATCATCCGCAAGAACTCGGAATCGTGGCGGTCGTTCCTCAGCC
TCAACGAGAAGTACCACGCTGGGAAACTCGATGAGAAACCCAGCCCACCAGGATACTGGGGCAACGAAGACGACGGACGGGTACTTCGCACGTACGTCCG
CAACGACCAGTACACCATCGAACTCGGGGAACGGTCCCGACTCGAAGTCCCGATTGGCTCCGAACTCAAAGACGAACTCGGCTTGAACCGGAATCAGCGT
CTCCGTGTTGAGGTGTCGGGTGACCCGAAGTGGAGCGGCGAGCAGGGCCGATTAGAACTCGTGTACGACGAACTCGCAGATACATTCAGGGCTTTTCAAC
CAGTCACTGTTGATGATTCTCAACTGGATTCACCACTGGCTTCCCACGAAGCCGCTCTGGACGTAGGTGCGAACAACCTCGTCGCCTGCACGGTTTCGAC
AGGGTCTCAATACCTATACAAAGGGCGCGGGCAATTCGAGCGGTTCCGCGAAACGACGCACCGAATCGCAGAACTGCAATCACGCCTCGAAGGCCAACGG
AAGACCAGTAAGCGTATCGACTGTTTGTACCGTAAGCGCACGAGTCGTCGAAACCACGCGCAGGACAGCCTTGTCCGAGACCTCGTGGAGCGACTCTACG
ACGAGGGTGTCTCCACGCTGTACGTAGGTGATATCAAAGGTGTCTTATCGACGCATTGGTCGGCTCGTGTGAACGAGAAGACGCATAACTTCTGGGCGTA
TCGACGGTTCATCAACCGCCTCGAATGCGTGTGCGAAGAATATGGTATCACGGTTGAGGAGGAATCCGAGGTGTGGACGAGTCAGGAATGTCCTGAATGC
GGTGAACGTGATGACACGGTTCGTCATGAGGATACGCTGACGTGTCCGTGTGGGTTCGAGGGGCACGCTGATTTGGTAGCGTCCAAATCGTTCTTGAGAC
GGCAGACCACTGCAGTCGGGTCGATGGCACGGCCCGTGTACCTCAAGTGGAACAATCACGATTGGCGGGAACACCACAGCCCGCCCTCCCTCGTGGAGAC
AACAGCCAACGAGGCGTACACAAACCAGAGTACCACTGTTCACGGTGGGAATATCGCTCTCGGGGACTCAGACGACTGAGACTCCCACGAGAGGAATCCT
CGCGCTTCAGCGCGGGGAGGATGTCAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1341 bp	446 aa	139	1479	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRANQFNVRPRFTEEREVFKRWLDASASLWNETNYARRQAFLSGSNESVWDAETGALGGKYKAALSSSVAQQIIRKNSESWRSFLSLNEKYHAGKLDEK
PSPPGYWGNEDDGRVLRTYVRNDQYTIELGERSRLEVPIGSELKDELGLNRNQRLRVEVSGDPKWSGEQGRLELVYDELADTFRAFQPVTVDDSQLDSPL
ASHEAALDVGANNLVACTVSTGSQYLYKGRGQFERFRETTHRIAELQSRLEGQRKTSKRIDCLYRKRTSRRNHAQDSLVRDLVERLYDEGVSTLYVGDIK
GVLSTHWSARVNEKTHNFWAYRRFINRLECVCEEYGITVEEESEVWTSQECPECGERDDTVRHEDTLTCPCGFEGHADLVASKSFLRRQTTAVGSMARPV
YLKWNNHDWREHHSPPSLVETTANEAYTNQSTTVHGGNIALGDSDD

Blast result :

Comments

ISHru1 is 72% (TnpB) aa similar to ISNamo21.

References

1] Pfeiffer, F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Davenport,K., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2011) Direct submission GenBank.