ISBvi7

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP000615 ND Burkholderia vietnamiensis
Burkholderia vietnamiensis G4
Burkholderia multivorans DDS 15A-1
DNA section
IS Length : 1177 bp

Ends


IR Length : 18/28

IRL : GGACTTTTTCAGTATGAAAGGAGCCGTACGATGAAACGCAAGCGCTTCAG
IRR : GGTTTTCAGTGCCAAAGGCAATGTTAGTCTTCGGCGTTCTGTTGGTCCGT

Insertion site


Left flankDirect repeatRight flankDR Length
AGTTTTCGGCGGGACCGTTC0

DNA sequence

GGACTTTTTCAGTATGAAAGGAGCCGTACGATGAAACGCAAGCGCTTCAGTGTCGAGCAGATTGTGGCGGTGTTGAAACAGGCCGAAGCAGGCATGCCGG
TGGCGGAACTGATTCGTCACGTGGGCATTTCGGAGCAAACCTTCTATCGCTGGAAGAAGGTGTATGCCGGTTTGCAGACGGACCAGGTGCGGCAGTTCAA
GCAGCTTCAAGACGAGAACGCCCAACTGAAGAAGGTGGTCGCGGAGCTGACGTTGGACAAGGCGATGCTGACCGACGTATTGAAAAAAAAGTGGTGAAGT
CCTCGCCGAGGCGCCACGTCGTGGATTATCTAAAGTCCGCCTATGGAGTAGCAGAGCGGCGTGCCTGTCGTCTGGTGGCCATATCGCGCTCGGTGTACCA
GTATCGCAGTCACCGCGACCCGGAGACTGCATTGCGGCAGCGCATGTGTGAAATCGCGGCGACGCGGGTACGCTATGGTTACCGCAAGATACGTGTGCTG
CTGCTACGGGAGGGCTATCAGGTAAGCAAGAATCGGCTGTACCGTCTGTATCGTGAGGAAGGGCTGTCGCTGCGCTACAGACCAAATCGCAAGCGACGTG
CACAGATGAGCCGCCCGGCACGTGCAAAGTCGACGGCGGCAAATCAGGCGTGGAGCTTGGACTTCGTCGCCGACCAGCTTTCGAACGGCCAGAGGTTCCG
CGCGCTGACCATCATCGACGTCTTTACGCGCGAAGCACTAGCCATCGATGTTGGCCAGCGACTGAGCGCTTCAGATGTCGTGCGTGTACTGGATGAGCTG
AGGAGCAAACGCGGAGCGCCCCGCACGCTGTTTTGTGACAACGGCTCGGAATTTACGAGTCAGGTGATGGACCTGTGGGCTTACCATCACAAGGTCGAGA
TTGCCTTCAGCCGGCCCGGCAAGCCAACGGATAACGCATTCGTGGAGTCGTTCAACGGCACGCTACGCGATGAATGCCTGAACGTGCACTGGTTCACATC
GCTGGCCGACGCCAGGGAGCAAATTGAACGATGGCGGGTCGAATACAACGAGAGTCGGCCTCACAGGGCTCTCGATGAAGTGCCGCCTGCCGAATACGTC
CGTCAGTTGGGCCTGCAGGCCCAACTGACGGACCAACAGAACGCCGAAGACTAACATTGCCTTTGGCACTGAAAACC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa31297+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKRKRFSVEQIVAVLKQAEAGMPVAELIRHVGISEQTFYRWKKVYAGLQTDQVRQFKQLQDENAQLKKVVAELTLDKAMLTDVLKKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1137 bp378 aa181154+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KEPYDETQALQCRADCGGVETGRSRHAGGGTDSSRGHFGANLLSLEEGVCRFADGPGAAVQAASRRERPTEEGGRGADVGQGDADRRIEKKVVKSSPRRH
VVDYLKSAYGVAERRACRLVAISRSVYQYRSHRDPETALRQRMCEIAATRVRYGYRKIRVLLLREGYQVSKNRLYRLYREEGLSLRYRPNRKRRAQMSRP
ARAKSTAANQAWSLDFVADQLSNGQRFRALTIIDVFTREALAIDVGQRLSASDVVRVLDELRSKRGAPRTLFCDNGSEFTSQVMDLWAYHHKVEIAFSRP
GKPTDNAFVESFNGTLRDECLNVHWFTSLADAREQIERWRVEYNESRPHRALDEVPPAEYVRQLGLQAQLTDQQNAED

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1124 bp374 aa311154+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRKRFSVEQIVAVLKQAEAGMPVAELIRHVGISEQTFYRWKKVYAGLQTDQVRQFKQLQDENAQLKKVVAELTLDKAMLTDVLKKKVVKSSPRRHVVDY
LKSAYGVAERRACRLVAISRSVYQYRSHRDPETALRQRMCEIAATRVRYGYRKIRVLLLREGYQVSKNRLYRLYREEGLSLRYRPNRKRRAQMSRPARAK
STAANQAWSLDFVADQLSNGQRFRALTIIDVFTREALAIDVGQRLSASDVVRVLDELRSKRGAPRTLFCDNGSEFTSQVMDLWAYHHKVEIAFSRPGKPT
DNAFVESFNGTLRDECLNVHWFTSLADAREQIERWRVEYNESRPHRALDEVPPAEYVRQLGLQAQLTDQQNAED

 

Blast result :
Comments
ISBvi7 is 80% aa similar to ISBcen22. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift. The terminal inverted repeat are not expected ends for an IS3 family members.
References
1] ISfinder submission (2015)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2005) Direct submission GenBank.