ISPko5
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
ND | Paracoccus kondratievae | Paracoccus kondratievae NCIBM 13773 |
DNA section
IS Length : 851 bp
Ends
IR Length : 15
IRL : GGTTGTGTTGACAAAAAGGATTCCCCTGCGCGTCTGGCTGTGATTCAAGA
IRR : GGTCATGTTTACAAATGGCGAAGCCAGAGCCTGATGCAGGCGATGTCGAT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GCACGCCGGC | TA | GATCGACGGC | 2 |
AAGATCAGAT | TA | GTATTGAGAG | 2 |
GCACGCCGGC | TA | GATCGACGGC | 2 |
GCTTACTTGC | TA | CGGCTCAATA | 2 |
CGGTGTCGCC | TA | GGCCCGCCAG | 2 |
DNA sequence
GGTTGTGTTGACAAAAAGGATTCCCCTGCGCGTCTGGCTGTGATTCAAGATCATCTCTACGTGGGAGGTGACCTTGGCACGCAATCTGATGTCCGACGAC
GAATGGGCATTCTTTGAGGGCTTCATCCGAGCCGTCCGGCATCCGAACGGGCGCAAGCCTGCGGACCATCGACTTGTTCTGGATGGCATTTTCTGGGTCG
CGCGGACCGGTGCGCCCTGGCGGGACCTGCCAGAGGAGTTTGGCAAGTGGTCATCTGTCTACCGGCAGTTCCGGCGCTGGACGCTCGCCGGGCTGTGGGA
GGACATCCTGGATGCCTTGAACCACGCGAAGCTCGCGCCCGACAAGCTCCAGATGATCGACAGCACTGTGATCAGGGCCCACCACCATGCGGCCGGCGCA
AAAGGGGGACTCCGAAAGAGGCTCCTGGCCATTCGAGAGGTGGGTTCTCGACCAAGATCCATCTCCGCGTCAACGGCGCTGGCCTCCCGATAAGGACCGA
GATCACGCCCGGGCAGGATTCAGACTACACGGGCTATGATCTGGTGATGGCCGACAACCTTCCGCAGCCCGCCGTGTTGGTCGCCGACAGGGGCTATGAC
TCTGATAAAATTAGAGAAGACATCGAGAGCCGTAACGCGCTGCCCATGATCCCCATGCGAAAGAACCGCAAGGTGCGCAAGGTCGTCGACATGACCATCT
ACACCTTGCGCAACATGGTCGAGCGTTGTTTCAACAAGCTCAAGAACAGTCGCCGCCTGGCAACACGCTACGACAAAACCGCAGACAGTTTCCTCGGCTT
CATCGACATCGCCTGCATCAGGCTCTGGCTTCGCCATTTGTAAACATGACC
GAATGGGCATTCTTTGAGGGCTTCATCCGAGCCGTCCGGCATCCGAACGGGCGCAAGCCTGCGGACCATCGACTTGTTCTGGATGGCATTTTCTGGGTCG
CGCGGACCGGTGCGCCCTGGCGGGACCTGCCAGAGGAGTTTGGCAAGTGGTCATCTGTCTACCGGCAGTTCCGGCGCTGGACGCTCGCCGGGCTGTGGGA
GGACATCCTGGATGCCTTGAACCACGCGAAGCTCGCGCCCGACAAGCTCCAGATGATCGACAGCACTGTGATCAGGGCCCACCACCATGCGGCCGGCGCA
AAAGGGGGACTCCGAAAGAGGCTCCTGGCCATTCGAGAGGTGGGTTCTCGACCAAGATCCATCTCCGCGTCAACGGCGCTGGCCTCCCGATAAGGACCGA
GATCACGCCCGGGCAGGATTCAGACTACACGGGCTATGATCTGGTGATGGCCGACAACCTTCCGCAGCCCGCCGTGTTGGTCGCCGACAGGGGCTATGAC
TCTGATAAAATTAGAGAAGACATCGAGAGCCGTAACGCGCTGCCCATGATCCCCATGCGAAAGAACCGCAAGGTGCGCAAGGTCGTCGACATGACCATCT
ACACCTTGCGCAACATGGTCGAGCGTTGTTTCAACAAGCTCAAGAACAGTCGCCGCCTGGCAACACGCTACGACAAAACCGCAGACAGTTTCCTCGGCTT
CATCGACATCGCCTGCATCAGGCTCTGGCTTCGCCATTTGTAAACATGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
426 bp | 141 aa | 68 | 493 | + | No |
Description : First part of the transposase
ORF sequence :
VTLARNLMSDDEWAFFEGFIRAVRHPNGRKPADHRLVLDGIFWVARTGAPWRDLPEEFGKWSSVYRQFRRWTLAGLWEDILDALNHAKLAPDKLQMIDST
VIRAHHHAAGAKGGLRKRLLAIREVGSRPRSISASTALASR
VIRAHHHAAGAKGGLRKRLLAIREVGSRPRSISASTALASR
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
549 bp | 182 aa | 295 | 843 | + | No |
Description : Second part of the transposase
ORF sequence :
VGGHPGCLEPREARARQAPDDRQHCDQGPPPCGRRKRGTPKEAPGHSRGGFSTKIHLRVNGAGLPIRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRG
YDSDKIREDIESRNALPMIPMRKNRKVRKVVDMTIYTLRNMVERCFNKLKNSRRLATRYDKTADSFLGFIDIACIRLWLRHL
YDSDKIREDIESRNALPMIPMRKNRKVRKVVDMTIYTLRNMVERCFNKLKNSRRLATRYDKTADSFLGFIDIACIRLWLRHL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
776 bp | 258 aa | 68 | 843 | + | Yes |
Chemistry : DDE
ORF sequence :
VTLARNLMSDDEWAFFEGFIRAVRHPNGRKPADHRLVLDGIFWVARTGAPWRDLPEEFGKWSSVYRQFRRWTLAGLWEDILDALNHAKLAPDKLQMIDST
VIRAHHHAAGAKRGTPKEAPGHSRGGFSTKIHLRVNGAGLPIRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRGYDSDKIREDIESRNALPMIPMRKN
RKVRKVVDMTIYTLRNMVERCFNKLKNSRRLATRYDKTADSFLGFIDIACIRLWLRHL
VIRAHHHAAGAKRGTPKEAPGHSRGGFSTKIHLRVNGAGLPIRTEITPGQDSDYTGYDLVMADNLPQPAVLVADRGYDSDKIREDIESRNALPMIPMRKN
RKVRKVVDMTIYTLRNMVERCFNKLKNSRRLATRYDKTADSFLGFIDIACIRLWLRHL
Blast result :
Comments
ISPko5 is 97% aa similar to ISPpa6.
ISPko5 was identified by in silico nucleotide sequence analysis of Paracoccus kondratievae plasmid pKON1 (95 kb). This IS is present in 5 copies in the pKON1 genome (2 copies of ISPko5 and 3 isoforms: ISPko5a, ISPko5b and ISPko5c, one copy each).
ISPko5 was identified by in silico nucleotide sequence analysis of Paracoccus kondratievae plasmid pKON1 (95 kb). This IS is present in 5 copies in the pKON1 genome (2 copies of ISPko5 and 3 isoforms: ISPko5a, ISPko5b and ISPko5c, one copy each).
References
1] Czarnecki J. (2015) Direct submission