ISPko3
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Paracoccus kondratievae | Paracoccus kondratievae NCIBM 13773 plasmid pKON1 |
DNA section
IS Length : 1050 bp
Ends
IR Length : 17
IRL : GGCTCTTTTGCACAAATGGCTGAGGGGATTCATCATGGGAATCCAGCGTG
IRR : TGTCCCTTTCCCCGGACGGATTTATCCCACAGTCTCTGTGACGGGTATGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCGCTGCAT | CAAGAAAGGC | 0 |
DNA sequence
GGCTCTTTTGCACAAATGGCTGAGGGGATTCATCATGGGAATCCAGCGTGATAGCTGGATGGCATGAGCAGACCCACACCCCCCGCCTACAAGACCCGGA
ACTGGCCGAGCTACAATGAAGCGCTCAAGCGCCGCGGCTCGCTGACGATCTGGTTCGATCCCGAGATGATCTGGGCGGCCGCGCCGACCGGCAAGCGTGG
TCGGCAGCCCAGCTATAGTGACGCTGCGGTCCAGACCTGCCTGACGATGAAGGTGCTGTTCGGCATGGCGCTCCGCCAGACCACCGGGTTCGTGGAAAGC
CTGCCGCAGTTGATCGGTCTGGACTGGGCGGTGCCCGATTTCAGCACCCTCAGCCGACGCCAGAAGACCTTGAAGGTCAACATCCCCTATCGCGGCTCGC
AGGGCCCGCTGCACCTTCTGATCGACAGCACCGGGATCAAGGTCGAGGGCGAAGGCGAGTGGAACGCGCGCAAGCATGGCGGCCCGAAACGACGTATCTG
GCGCAAGGTCCACCTCGGGATCGACGAGAAAACACTGGAAATCAGGGCGGTCGAGTTCACCAGTAGCGACGTAGGCGACGCACCGATGTTGCCCGAACTG
CTCGACCAGATCCCACCCGATCAAGAGATCGCCAGCGTTACCGCCGATGGCGCATACGACACCCGAAAGTGCCACGACGCCATCGCCGCCCGCGCCGCTG
CGGCCATCATTCCTGTGCGGAAGAACGCCAAGCCCTGGAAGAAAACTTCAGCCGGAGCCGTAGCACGGAACGATATCTTGCGAACCACCAAATACCTCGG
CCGGACAATCTGGCGACGATGGAGCGCATATCATCGCCGAAGTCGTGCAGAGACAAAAATGCACTGTATGAAACTGCTGGGCCAGCGCCTGACCGCGAGG
GAGTTCGACTGCCAGGTCGCAGAACTCCATATCCGCATGGCTGTCATGAACGGCTACACAGCGCTCGGCATACCCGTCACAGAGACTGTGGGATAAATCC
GTCCGGGGAAAGGGACA
ACTGGCCGAGCTACAATGAAGCGCTCAAGCGCCGCGGCTCGCTGACGATCTGGTTCGATCCCGAGATGATCTGGGCGGCCGCGCCGACCGGCAAGCGTGG
TCGGCAGCCCAGCTATAGTGACGCTGCGGTCCAGACCTGCCTGACGATGAAGGTGCTGTTCGGCATGGCGCTCCGCCAGACCACCGGGTTCGTGGAAAGC
CTGCCGCAGTTGATCGGTCTGGACTGGGCGGTGCCCGATTTCAGCACCCTCAGCCGACGCCAGAAGACCTTGAAGGTCAACATCCCCTATCGCGGCTCGC
AGGGCCCGCTGCACCTTCTGATCGACAGCACCGGGATCAAGGTCGAGGGCGAAGGCGAGTGGAACGCGCGCAAGCATGGCGGCCCGAAACGACGTATCTG
GCGCAAGGTCCACCTCGGGATCGACGAGAAAACACTGGAAATCAGGGCGGTCGAGTTCACCAGTAGCGACGTAGGCGACGCACCGATGTTGCCCGAACTG
CTCGACCAGATCCCACCCGATCAAGAGATCGCCAGCGTTACCGCCGATGGCGCATACGACACCCGAAAGTGCCACGACGCCATCGCCGCCCGCGCCGCTG
CGGCCATCATTCCTGTGCGGAAGAACGCCAAGCCCTGGAAGAAAACTTCAGCCGGAGCCGTAGCACGGAACGATATCTTGCGAACCACCAAATACCTCGG
CCGGACAATCTGGCGACGATGGAGCGCATATCATCGCCGAAGTCGTGCAGAGACAAAAATGCACTGTATGAAACTGCTGGGCCAGCGCCTGACCGCGAGG
GAGTTCGACTGCCAGGTCGCAGAACTCCATATCCGCATGGCTGTCATGAACGGCTACACAGCGCTCGGCATACCCGTCACAGAGACTGTGGGATAAATCC
GTCCGGGGAAAGGGACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 64 | 996 | + | No |
Chemistry : DDE
ORF sequence :
MSRPTPPAYKTRNWPSYNEALKRRGSLTIWFDPEMIWAAAPTGKRGRQPSYSDAAVQTCLTMKVLFGMALRQTTGFVESLPQLIGLDWAVPDFSTLSRRQ
KTLKVNIPYRGSQGPLHLLIDSTGIKVEGEGEWNARKHGGPKRRIWRKVHLGIDEKTLEIRAVEFTSSDVGDAPMLPELLDQIPPDQEIASVTADGAYDT
RKCHDAIAARAAAAIIPVRKNAKPWKKTSAGAVARNDILRTTKYLGRTIWRRWSAYHRRSRAETKMHCMKLLGQRLTAREFDCQVAELHIRMAVMNGYTA
LGIPVTETVG
KTLKVNIPYRGSQGPLHLLIDSTGIKVEGEGEWNARKHGGPKRRIWRKVHLGIDEKTLEIRAVEFTSSDVGDAPMLPELLDQIPPDQEIASVTADGAYDT
RKCHDAIAARAAAAIIPVRKNAKPWKKTSAGAVARNDILRTTKYLGRTIWRRWSAYHRRSRAETKMHCMKLLGQRLTAREFDCQVAELHIRMAVMNGYTA
LGIPVTETVG
Blast result :
Comments
ISPko3 is 90% aa similar to ISSisp2.
ISPko3 was identified by in silico nucleotide sequence analysis of Paracoccus kondratievae plasmid pKON1 (95 kb). This IS is present in 1 copy in the pKON1 genome.
ISPko3 was identified by in silico nucleotide sequence analysis of Paracoccus kondratievae plasmid pKON1 (95 kb). This IS is present in 1 copy in the pKON1 genome.
References
1] Czarnecki J. (2015) Direct submission