ISfinder Mirror Site

ISHhu11

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
LN831304	ND	Halobacterium hubeiense	Halobacterium hubeiense

DNA section

IS Length : 1622 bp

Ends

Left end : CGGACGGAGTACGCCGTGACTCAGGGCTTGACTCCGAGAGTGAAGGCCGTACGTTCCGCTAAGGAACAGGTATGCGTGGTTCGTTCGCTTCTCGCTTATA II struct. : Yes
Right end : GTGACCCACGACGCCGTTCGAGGCTATCAGACCGATGGCGTCGCGGGAGTGTCCGACTAATCCACGGGAAGTCTCGAGGACCGTTCTCGAGACAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CCTCACGCATCG	TCTA	GGCGCCGAACGG	tcac

DNA sequence

CGGACGGAGTACGCCGTGACTCAGGGCTTGACTCCGAGAGTGAAGGCCGTACGTTCCGCTAAGGAACAGGTATGCGTGGTTCGTTCGCTTCTCGCTTATA
TGGAGGATCAGCCACCGATCGAATTAAGTATATCCAAAGGATATACACCGATATGGCGAATCGGTTTCAAATCGACGGCGAGGAAGTTCTCGACGGCCAA
GTCAAGGAGTTCGGGAACAGCGCCCACGTCACCGTCCCCAAACGCTGGCGTGGGGCTAACGTGAAAGTCGTCCGTACCTCAGAACCAACCGAACAAGACG
AAGAATGACTGATTCACAGGCTCTCATCAAGACGCTGGACTTCCAGCTCGACATCCAGAGCGATAATGAGAGCCTGCTGTACGACGCCACCCTCGAAGCG
AGGTCGGTATACAACCAATCTATCCGACTCGCCAAGGAAGGTGTGGATTGGAACGTCATTCCCGACCGCGTGGCCGAGGACGCCAGCCTCGTGAAAAACA
CGATACAGCGCGTCGTCGCCAAAGCACTCGGTGCGATGGAGAACTACTACGAGTATGACGACTACAACAAACCCAGCCACACCAAAGACGGAACGTACCC
GCTCCGGGCGAACTACGAGGAGGGGTACAACCTGTCACTCACCGACGACGGCGACGTGGCGTTCCGGATCAGCGCGAAGCCCTACAAGCACGTCAAGGGC
GTCCTCGATGGTGACGACGCTCATCTCGACATTCTCAAGACCGCCCTCGAAAGCGATGCATGGACGATTGGGACGGCGGAAGCCCTGTTCCGAAACGGCA
ACGCCGAGCTGCACGTCAACGTAACTAGCTCCGAGGGGACCGTTCGAGACAAGGAAAATTCACGGACAGTCGTTGGCGTGGATGTCAACGAGGACAATGT
GGCGCTGACTGCACTCTCCAAGGGCAGTGTCGAGGACACAGTCGTTATCGAATTTCCGGACATCAAGTTCAAGCGCCACCGCTATTTCACGATGCGGAAG
CGCGTGCAGAACGCGGGGAAGGACAGCATCCACGACACGTTGGAAGGACGTGAGGAACGGTTTGTCCGCGACCGACTCCACAAAGTGAGCCGTCATATCG
TCGAGTGGAGCCAGCAGTTCGAGAAGCCGTGCATCGTCTTTGAAGACCTCAAAGAGATGCGCGATGGCCTCGACTACGGATGCCGGATGAACCGACGCTT
GCACCGCCTCCCGTTCCGCGCGCTTCAGCACTACACGTCCTACAAGGCAGCATTCGCGGGTATTCCGACGGCTTGGATCAATCCCGAATACACGAGCCAA
TGCTGTCCGATGTGCGGTCATACGGAACGATCGAACCGTCACAAGAAGCGGTTCAAGTGCCAGTCCTGTTCGCATCAAGACCACGCCGACCGGAGTGCAA
GCGTGAATATCGCCGTGAAAGGTATCGAAGAGCATCAAGAGTGGACTGTGCCTGCTCTCAACAGCCTTCCCACTGTGCGGACGGTGCGACGGCAGGCATC
GGGGGCCGTGGACGCCCCGACCGTGACCCACGACGCCGTTCGAGGCTATCAGACCGATGGCGTCGCGGGAGTGTCCGACTAATCCACGGGAAGTCTCGAG
GACCGTTCTCGAGACAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
156 bp	51 aa	153	308	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MANRFQIDGEEVLDGQVKEFGNSAHVTVPKRWRGANVKVVRTSEPTEQDEE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1272 bp	425 aa	311	1582	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTDSQALIKTLDFQLDIQSDNESLLYDATLEARSVYNQSIRLAKEGVDWNVIPDRVAEDASLVKNTIQRVVAKALGAMENYYEYDDYNKPSHTKDGTYPL
RANYEEGYNLSLTDDGDVAFRISAKPYKHVKGVLDGDDAHLDILKTALESDAWTIGTAEALFRNGNAELHVNVTSSEGTVRDKENSRTVVGVDVNEDNVA
LTALSKGSVEDTVVIEFPDIKFKRHRYFTMRKRVQNAGKDSIHDTLEGREERFVRDRLHKVSRHIVEWSQQFEKPCIVFEDLKEMRDGLDYGCRMNRRLH
RLPFRALQHYTSYKAAFAGIPTAWINPEYTSQCCPMCGHTERSNRHKKRFKCQSCSHQDHADRSASVNIAVKGIEEHQEWTVPALNSLPTVRTVRRQASG
AVDAPTVTHDAVRGYQTDGVAGVSD

Blast result :

Comments

ISHhu11 is 91% aa (ORFB) similar to ISHma7. The first ORF is a passenger gene.

References

1] F. Pfeiffer (2013) Direct submission
2] Jaakkola S.T., Pfeiffer F., Ravantti J.J., Guo Q., Liu Y., Chen X., Ma H., Yang C., Oksanen H.M., Bamford D.H. (2015) Environ Microbiol. doi: 10.1111/1462-2920.13130