ISPsp10
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ920388 | Y | Pseudomonas sp. | Pseudomonas sp. LM25 |
DNA section
IS Length : 945 bp
Ends
IR Length : 14/21
IRL : GGTTCTGTATGAAAACCTTTGAAACCTCACCGGAGCCTCTGGAAGCCTGA
IRR : TTAGGCACTGTACGAAAAGTAATGTCGTAAACACCGTCGCCAGCAAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATTCTATCC | TA | AAAGACCAAA | 2 |
DNA sequence
GGTTCTGTATGAAAACCTTTGAAACCTCACCGGAGCCTCTGGAAGCCTGATTTTCCGTAGAGTTCTCGCTTTTTAGGCGAAGGATTCTGCCATGTCCAAG
CGATACGAACTTTCCGATGCAGAATGGGAAATAGTCGCGGACCTCTTCACCGCACATCGACGTACAGGACGCCCAAGAGTCGATGATCGCTTGATGCTCA
ACGGCGTGTTGTGGGTGCTGTGCTCAGGCGCAACCTGGCGGGACATGCCTGAACGGTTCGGGCCTTGGTCGACGGTCTATCAACGATTTCGAGACTGGCG
AAACCAAGGAACCTTCAACAAGATGCTCAAGCGGTTACACCTCAAACTCAATGCACAGGGCCAGATTGACTTGACCACCTGGTGCATCGACTCAACCGCC
GTGAGGGCCACCCGTGCCTCTGCCGGAGCAGGCAAAAAAGGGGGGCTGAAGAACCAACGGACCACGCACTTGGACGAAGCCGGGGCGGGTTGACGACGAA
AATTCACATGGTCAGTGACGCCCGTGGCATCCCACTCCACTTCACGCTCTCAGGTGGTCACGCCAGTGACATATCCCATGCTCAGCGACTGCTGGACGGT
GTCCACATCCCTTTGAACCGAGGTAGGCCACGCAAGCGCTGCCGCTGGCTCCTGGCCGACAAAGGCTATGACGCAGAATCCTTGCGGCAATACTGCGACC
GCTATCGGATGCAGCCGGTGATTCCGCTGCGTGACATGAAACGCAGACCGAAGCCGGGCTTACCGCGCCTGTTTGACAAGCCCAAGTATCGGCAGCGGAA
CGCCATCGAACGGCTATTTGGTTGGCTGAAGGAGCACCGCCGCCTGGGTACACGCTACTGCAAGCTGGCGGAAAGCTTTGCTGCAATGGTCACGCTGGCT
TGCTGGCGACGGTGTTTACGACATTACTTTTCGTACAGTGCCTAA
CGATACGAACTTTCCGATGCAGAATGGGAAATAGTCGCGGACCTCTTCACCGCACATCGACGTACAGGACGCCCAAGAGTCGATGATCGCTTGATGCTCA
ACGGCGTGTTGTGGGTGCTGTGCTCAGGCGCAACCTGGCGGGACATGCCTGAACGGTTCGGGCCTTGGTCGACGGTCTATCAACGATTTCGAGACTGGCG
AAACCAAGGAACCTTCAACAAGATGCTCAAGCGGTTACACCTCAAACTCAATGCACAGGGCCAGATTGACTTGACCACCTGGTGCATCGACTCAACCGCC
GTGAGGGCCACCCGTGCCTCTGCCGGAGCAGGCAAAAAAGGGGGGCTGAAGAACCAACGGACCACGCACTTGGACGAAGCCGGGGCGGGTTGACGACGAA
AATTCACATGGTCAGTGACGCCCGTGGCATCCCACTCCACTTCACGCTCTCAGGTGGTCACGCCAGTGACATATCCCATGCTCAGCGACTGCTGGACGGT
GTCCACATCCCTTTGAACCGAGGTAGGCCACGCAAGCGCTGCCGCTGGCTCCTGGCCGACAAAGGCTATGACGCAGAATCCTTGCGGCAATACTGCGACC
GCTATCGGATGCAGCCGGTGATTCCGCTGCGTGACATGAAACGCAGACCGAAGCCGGGCTTACCGCGCCTGTTTGACAAGCCCAAGTATCGGCAGCGGAA
CGCCATCGAACGGCTATTTGGTTGGCTGAAGGAGCACCGCCGCCTGGGTACACGCTACTGCAAGCTGGCGGAAAGCTTTGCTGCAATGGTCACGCTGGCT
TGCTGGCGACGGTGTTTACGACATTACTTTTCGTACAGTGCCTAA
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 402 bp | 133 aa | 92 | 493 | + | No |
Description :
ORF sequence :
MSKRYELSDAEWEIVADLFTAHRRTGRPRVDDRLMLNGVLWVLCSGATWRDMPERFGPWSTVYQRFRDWRNQGTFNKMLKRLHLKLNAQGQIDLTTWCID
STAVRATRASAGAGKKGGLKNQRTTHLDEAGAG
STAVRATRASAGAGKKGGLKNQRTTHLDEAGAG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 576 bp | 191 aa | 370 | 945 | + | No |
Description :
ORF sequence :
LDHLVHRLNRREGHPCLCRSRQKRGAEEPTDHALGRSRGGLTTKIHMVSDARGIPLHFTLSGGHASDISHAQRLLDGVHIPLNRGRPRKRCRWLLADKGY
DAESLRQYCDRYRMQPVIPLRDMKRRPKPGLPRLFDKPKYRQRNAIERLFGWLKEHRRLGTRYCKLAESFAAMVTLACWRRCLRHYFSYSA
DAESLRQYCDRYRMQPVIPLRDMKRRPKPGLPRLFDKPKYRQRNAIERLFGWLKEHRRLGTRYCKLAESFAAMVTLACWRRCLRHYFSYSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 854 bp | 283 aa | 92 | 945 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKRYELSDAEWEIVADLFTAHRRTGRPRVDDRLMLNGVLWVLCSGATWRDMPERFGPWSTVYQRFRDWRNQGTFNKMLKRLHLKLNAQGQIDLTTWCID
STAVRATRASAGAGKRGAEEPTDHALGRSRGGLTTKIHMVSDARGIPLHFTLSGGHASDISHAQRLLDGVHIPLNRGRPRKRCRWLLADKGYDAESLRQY
CDRYRMQPVIPLRDMKRRPKPGLPRLFDKPKYRQRNAIERLFGWLKEHRRLGTRYCKLAESFAAMVTLACWRRCLRHYFSYSA
STAVRATRASAGAGKRGAEEPTDHALGRSRGGLTTKIHMVSDARGIPLHFTLSGGHASDISHAQRLLDGVHIPLNRGRPRKRCRWLLADKGYDAESLRQY
CDRYRMQPVIPLRDMKRRPKPGLPRLFDKPKYRQRNAIERLFGWLKEHRRLGTRYCKLAESFAAMVTLACWRRCLRHYFSYSA
Blast result :
Comments
ISPsp10 is 86% aa similar to ISPst8. The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. ISPsp10 has been identified by its transposition to entrapment vector pMAT1 (sacB cassette). A unique feature of ISPsp10 is the lack of a stop codon within ORFB encoding the terminal part of the transposase. ISPsp10
transposed into the sacB gene just upstream of a +TAA sequence (upon insertion the dinucleotide TA was duplicated); therefore, the stop codon in this case is provided by the target site of transposition. As judged from DNA hybridization analysis ISPsp10 is present in 4 copies in the genome of Pseudomonas sp. LM25. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM15.
transposed into the sacB gene just upstream of a +TAA sequence (upon insertion the dinucleotide TA was duplicated); therefore, the stop codon in this case is provided by the target site of transposition. As judged from DNA hybridization analysis ISPsp10 is present in 4 copies in the genome of Pseudomonas sp. LM25. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM15.
References
1] Szuplewska, M., and Bartosik, D. (2014) Direct submission.