ISPsp9

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KJ920387 Y Pseudomonas sp.
Pseudomonas sp. LM13
DNA section
IS Length : 1718 bp

Ends


IR Length : 18/21

IRL : CCCGGCTTATTCATGAGGCCGACCTGAAAACGAAGATGGCGAGTGGTTTT
IRR : CCCGCCTTATTCACGAGGGCGCTGATTGAGCGGTCTGAAGGTACTGGCTG

Insertion site


Left flankDirect repeatRight flankDR Length
TTTGTTTGCCGTAATGTTTACCGGA5

DNA sequence

CCCGGCTTATTCATGAGGCCGACCTGAAAACGAAGATGGCGAGTGGTTTTCTCTTGTAGAATCAGGATGTTCCTGCCAGAACACGATTCAAGAGGACCAC
TCGCCATGGGTGAAAGCCTATCCACCTGGACGCCCTCATGCAACGGCTCTGTCCGTGTTGAGCTGAGCGGCCAGCGCACCACCAGCGACAGCGGCGCCCT
GCTGCTGCGTGAAGCCCTCGACAACAGCGGCGTAATCGAGGCGCTGGAAGACAATCTGGTCGATCGACGCCACCCGCTGCGCATCCGTCATTCGCTGGCC
AGCCAGTTGCGTACCCTGGTGCTGCAGCGTGCGATGGGCTGGATCGATCTCAGCGATACCGACACGCTGCGCCGTGACCCGCTTTGGCAGTTGGCCTGCA
GCGATGCACGCGGCATGACACCCCTGGTCCAGGACCGGCCGTCTCAAGCCACGCTGTCGCGGCTGCTGAGCTGCCTCGGTCGTAACGACAATATCGATGC
CGTGCACGAAGGCCTGTTGCGGCTGGTGGTCTGGCGCCTGACCTCGCTGCAGAACGGTGAACGTCCCAAACAGCTGACGCTGGACATCGATGGCCTGCCG
ATCGAGGTGCATGGCCATCAGGGCGGGTCGGCCTATCATGGCCTTTACGGGGCCCGCATCTACTCGCCGCTGGTCGCATCGTTGGCGGAGACCGGCGACA
TGGTGGGCGGCCTGCTGCGCGAGGGCAACGCCGGCCCGGCAGAGAACGCCGACACCTGGATCCCGCATCTGGTACGACGACTCAACGAGAGCACCGGGGC
ACAGGTTCGTGTGCGCATCGACGCCGGTTTTACCGATAACGCGACGCTGGAGGCCCTGGAGGATCGGGAGATCGAATACCTGGGCCGGTTGCGCAGTCAC
ACCGGTCTACAGAAGCTGGCAGCACCGCTCCTGAAGCGGCCACCCGGCCGGCCACCCGAGCAGCCTCGCGAGTGGTGCCACGATCTGGAGTACCAAGCCG
GCTCCTGGTCGGCACCGCGGCGCGTAGTGCTGGTGGTGCAGGAACGCCCCGATGACCTGCTGCTGCATGCCTTCTTCCTGGTCACCAACCTCAGCAAGTT
CGACTGGCCGCCAGAGAAGGTCCTAGCACTCTATCGCAAGCGCGGCAGCGCCGAGGCGCACATGGGCGAGGTGAAATCAGCACTCGACGTGCATCTCTCC
TCGACCGACCGCGGCGCTTCCACCGTTCAAGAGGTGATGGCCCGCAACGAAGTGAGTCTGCTACTGAGTCTCTACGCCTACCAGGTCCTGCATGGCCTGC
GCTGCCTGCTGGAGCGGCAGACCCGGCAAGGCTGGAGCCTCAGCCGGATGCGCGAGCAGGTGCTGAAGGTCGCCGCCACGCTGAGGCTGCACGCTCGGCG
AATCACCGTTCATCTGGGGGCCGCCGCCGACAAGTGGTGGCCGACGCTGCTGAAGGGGCTGCCGAAGCTGACCGCACTGAGCTGACGCTCCTGCGAGACC
ACCACAGCATCAGAAAGCGAGCGACCGGAGCGGCGGTCGGCGATCACGGCTGCTTGGACGGTCGCAATTGATTCCAAAGAGCTATAAGTAAGTAGGAAAA
GGGCTGCGTTCTGGCATTACTCAACAGCCACTCGCGCTGAAGATGCGAATCCTGATAAGCAATGCCAACAGCCAGTACCTTCAGACCGCTCAATCAGCGC
CCTCGTGAATAAGGCGGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1419 bp472 aa671485+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFLPEHDSRGPLAMGESLSTWTPSCNGSVRVELSGQRTTSDSGALLLREALDNSGVIEALEDNLVDRRHPLRIRHSLASQLRTLVLQRAMGWIDLSDTDT
LRRDPLWQLACSDARGMTPLVQDRPSQATLSRLLSCLGRNDNIDAVHEGLLRLVVWRLTSLQNGERPKQLTLDIDGLPIEVHGHQGGSAYHGLYGARIYS
PLVASLAETGDMVGGLLREGNAGPAENADTWIPHLVRRLNESTGAQVRVRIDAGFTDNATLEALEDREIEYLGRLRSHTGLQKLAAPLLKRPPGRPPEQP
REWCHDLEYQAGSWSAPRRVVLVVQERPDDLLLHAFFLVTNLSKFDWPPEKVLALYRKRGSAEAHMGEVKSALDVHLSSTDRGASTVQEVMARNEVSLLL
SLYAYQVLHGLRCLLERQTRQGWSLSRMREQVLKVAATLRLHARRITVHLGAAADKWWPTLLKGLPKLTALS

 

Blast result :
Comments
ISPsp9 is 47% aa similar to ISApr8. ISPsp9 has been identified by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, ISPsp9 is present in 4 copies in the genome of Pseudomonas sp. LM13. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2.
References
1] Szuplewska, M., and Bartosik, D. (2014) Direct submission.