ISPsp8
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ920386 | Y | Pseudomonas sp. | Pseudomonas sp. LM13 |
DNA section
IS Length : 1113 bp
Ends
IR Length : 22/27
IRL : GGCGGCGGCAAAAACTGAGTGCAACACCTGACCTTTCCGGTACGGTGCTG
IRR : GGCGGTTGCAGGAGCTGAGTGCAACACGGTCATTGAATTGAAACTGGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGGGATTTG | CAG | CATATCATGG | 3 |
DNA sequence
GGCGGCGGCAAAAACTGAGTGCAACACCTGACCTTTCCGGTACGGTGCTGCCCCTATGTCATATACCGAACTCAGCGTTGAAGAACGCGCCACCATCCAA
ATCGGCCACGCCCAGGGCTTCAGCCTGCGCAGGATTGCTCGCCTGATCAATAGGTCTCCTTCGACCATCAGTCGAGAGCTGCGCCGCAATCGAGATGTCT
GTGGCAGCTACTCGGCCCGCGCGGCCCAGGAGCAGATGAAGGCCCGCCGCCAAGTCTGTCGACCGAGCCGAAAGCTGCTGCCGGGGAGCGAGCGCTTCGA
ACTGGTGGTTCATATGCTGCGTGAGCGTTTGTCTCCCGAACAGATTGCCGGCAAGCTGCGCCACATGAACATACCTAGCCTCAGAGATGCCTACGTCTGT
CGCGAGACGATCTACAACGCGATCTATGCCCTGCCAGTGGGTGAGCTGCGTAAGGAGCTGATCATCTGTCTGCGCCAAGGCAAGACGACGCGCCGGCCGC
GCTCTGGTGGCGTGGATCGGCGCGGCCAGATCCCCGAGATGGTCAGTATTCATGTGCGCCCGCCGGAGATTGAAGACAGGCTGATGCCGGGGCATTGGGA
AGGCGACCTCATCAAGGGTAAGGCCAACGCCTCGTCCGTCGGTACGCTGGTGGAACGCACCAGTGGGTACCTGATGTTGGTGAAGATGAACGACGCGACG
GCGACTTCGGCGCTGGAAGGCTTCAGCGCCGCGCTCAATAGCATGCCGCTGGAGATGCGCAAGAGCATGACTTACGACCAGGGCCGGGAGATGGCGCGAC
ACGCCGAGATCACCCAAAGAACCGGCGTGGCGATCTACTTCTGCGACCCGCACAGCCCCTGGCAGCGCGGCAGCAACGAAAACATCAACGGCCTGATTCG
CCAGTACTTGCCCAAGGGCACAGACCTGTCGGTACATAGCCAGGAGGAGCTGGACGCCATTGCGCTGCAACTGAACATGCGACCGCGCAAGCGCTTCGAC
TTCAAATGCCCAATCGAAGTTATGGACGAGGTGATGCAGAAGGCCATGGCTATGCGGCATGATGCTCCAGTTTCAATTCAATGACCGTGTTGCACTCAGC
TCCTGCAACCGCC
ATCGGCCACGCCCAGGGCTTCAGCCTGCGCAGGATTGCTCGCCTGATCAATAGGTCTCCTTCGACCATCAGTCGAGAGCTGCGCCGCAATCGAGATGTCT
GTGGCAGCTACTCGGCCCGCGCGGCCCAGGAGCAGATGAAGGCCCGCCGCCAAGTCTGTCGACCGAGCCGAAAGCTGCTGCCGGGGAGCGAGCGCTTCGA
ACTGGTGGTTCATATGCTGCGTGAGCGTTTGTCTCCCGAACAGATTGCCGGCAAGCTGCGCCACATGAACATACCTAGCCTCAGAGATGCCTACGTCTGT
CGCGAGACGATCTACAACGCGATCTATGCCCTGCCAGTGGGTGAGCTGCGTAAGGAGCTGATCATCTGTCTGCGCCAAGGCAAGACGACGCGCCGGCCGC
GCTCTGGTGGCGTGGATCGGCGCGGCCAGATCCCCGAGATGGTCAGTATTCATGTGCGCCCGCCGGAGATTGAAGACAGGCTGATGCCGGGGCATTGGGA
AGGCGACCTCATCAAGGGTAAGGCCAACGCCTCGTCCGTCGGTACGCTGGTGGAACGCACCAGTGGGTACCTGATGTTGGTGAAGATGAACGACGCGACG
GCGACTTCGGCGCTGGAAGGCTTCAGCGCCGCGCTCAATAGCATGCCGCTGGAGATGCGCAAGAGCATGACTTACGACCAGGGCCGGGAGATGGCGCGAC
ACGCCGAGATCACCCAAAGAACCGGCGTGGCGATCTACTTCTGCGACCCGCACAGCCCCTGGCAGCGCGGCAGCAACGAAAACATCAACGGCCTGATTCG
CCAGTACTTGCCCAAGGGCACAGACCTGTCGGTACATAGCCAGGAGGAGCTGGACGCCATTGCGCTGCAACTGAACATGCGACCGCGCAAGCGCTTCGAC
TTCAAATGCCCAATCGAAGTTATGGACGAGGTGATGCAGAAGGCCATGGCTATGCGGCATGATGCTCCAGTTTCAATTCAATGACCGTGTTGCACTCAGC
TCCTGCAACCGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 56 | 1084 | + | No |
Chemistry : DDE
ORF sequence :
MSYTELSVEERATIQIGHAQGFSLRRIARLINRSPSTISRELRRNRDVCGSYSARAAQEQMKARRQVCRPSRKLLPGSERFELVVHMLRERLSPEQIAGK
LRHMNIPSLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPEMVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVE
RTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTYDQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELD
AIALQLNMRPRKRFDFKCPIEVMDEVMQKAMAMRHDAPVSIQ
LRHMNIPSLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPEMVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVE
RTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTYDQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELD
AIALQLNMRPRKRFDFKCPIEVMDEVMQKAMAMRHDAPVSIQ
Blast result :
Comments
ISPsp8 is 95% aa similar to ISPsp7. ISPsp8 has been identified by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPsp8 is present in 2 copies in the genome of Pseudomonas sp. LM13. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina LM7, Pseudomonas sp. LM8, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM13, Pseudomonas sp. LM14, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2
References
1] Szuplewska, M., and Bartosik, D. (2014) Direct submission.