ISMyma10
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Mycobacterium marinum | Mycobacterium marinum 324-958 |
DNA section
IS Length : 1352 bp
Ends
IR Length : 25/31
IRL : TGAAGCATCCCCGGTTTCATGCACACCTCGTTACGTGTGACCGGTGTGCT
IRR : TGAACCGTCCCCGGTTTTATGCGCACTTCCTTTGTTGGGAAGGATGGCGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTGCGCACC | CCCGCATCCC | 0 | |
| GCATCGCGAA | TCGGCGACCC | 0 |
DNA sequence
TGAAGCATCCCCGGTTTCATGCACACCTCGTTACGTGTGACCGGTGTGCTTTCCGGCCTGGAGTCGAGCGTAGTACTCGGCCTCGGCTTCCGCGGGTGGG
ATGCGGCCCAGGCGGTGCATGAGCCGCCGCTCGTTGTACCAGCTAACCCACGCAGAGGTCATGTCCTCCAGGTCGGGCAGGGTGCGGATCGGACCGACGC
GAAACGGGGAGCCAGGGCGAACGCACTCGGTCTTGTAGAGCCCGATCGTGGTTTCGCACAGCGCGTTGTCCAGGGCGTCGCCGACGGTACCGATCGACGG
GATCAGCCCCTCCAACATCAACGTATCGCCGTAATGTATTGCGGTGTAATGACTTCCAGCGTCGCTGTGATGGATTGTGGCATCGAACGGATGGCCCTGA
CGGGCACGGAAGACCGCCGCGTGGCGCAGCGCGCGCTCGACGAAACCGGCGTCCTTGGTCATCGAGCAGTCCCAGCCCGGGATCAGCCCGGCGTAGGCGT
CGACGACGAACGCGGTGTAGCCGAACCCACCGGTGTCCAGCGGGACGTAGGTGAAGTCAGCTACGTCGAGCAAATTGGGTGCGGCAACGCGCCACTTGCG
GCCCACCAGGTCTGGCGCCCGAGCCGCGGCCGGGTCGCGCTCGGTGGTGCGCGGCGGGCGGCGAGCGCGGGTGACACCTCGCCAGCCGTTGTGCCGCATG
ATCCGTTCCACCGTGCGCCGCGCCACCGGGATGCCCTGCCGCTGCAGGTGCGCCCACATCTTCAGGCTGCCGTACAGGCTCTCCGGCGGGCGTTTCCCGT
CGGCGTCAGGTTCGTAGTAGCCGGCCAGGATCTCGGTGATCGTGGTGTCCCACAGGGCCCGTTTCGACGGCGCCGACGAGCGGTGGGCCCAGTAGGTGCG
CGGGGCGATCGCCACGCCCTGGATCCCCAGGGCGCGGCAGATCGGTACGACCCCGAACCGCTCCTTGTGTTCGTCGATGAACGCACAGATCAGCGGTGTA
GCGGGTCGCACTCCCGCGCGAAGAAAGTCGTTGCGGCCTTGAGGATTTCGATCGTCGCCTCAAGCTCCCTGGTCTTGCGGCGTAGCTCCCGCAGCTCCCG
GCTCTCCTCGCTGGATATCCCTGCCGCCTCACCGGCATCGACCTCGGCCTGGCGCACCCACTTGCGCAGCGTCTCCGTGTTCATCCCCAGCCGGCCCGCG
ATCGCCTTCATCGCCGCCCACTCTGAGTCGTAATCATCGCGGTGCTCGCGGACCAGCCGCACCGCCCTGGCCTTGGTGTTCTCGTCATACTTGCTCGGCA
TCGCGCCATCCTTCCCAACAAAGGAAGTGCGCATAAAACCGGGGACGGTTCA
ATGCGGCCCAGGCGGTGCATGAGCCGCCGCTCGTTGTACCAGCTAACCCACGCAGAGGTCATGTCCTCCAGGTCGGGCAGGGTGCGGATCGGACCGACGC
GAAACGGGGAGCCAGGGCGAACGCACTCGGTCTTGTAGAGCCCGATCGTGGTTTCGCACAGCGCGTTGTCCAGGGCGTCGCCGACGGTACCGATCGACGG
GATCAGCCCCTCCAACATCAACGTATCGCCGTAATGTATTGCGGTGTAATGACTTCCAGCGTCGCTGTGATGGATTGTGGCATCGAACGGATGGCCCTGA
CGGGCACGGAAGACCGCCGCGTGGCGCAGCGCGCGCTCGACGAAACCGGCGTCCTTGGTCATCGAGCAGTCCCAGCCCGGGATCAGCCCGGCGTAGGCGT
CGACGACGAACGCGGTGTAGCCGAACCCACCGGTGTCCAGCGGGACGTAGGTGAAGTCAGCTACGTCGAGCAAATTGGGTGCGGCAACGCGCCACTTGCG
GCCCACCAGGTCTGGCGCCCGAGCCGCGGCCGGGTCGCGCTCGGTGGTGCGCGGCGGGCGGCGAGCGCGGGTGACACCTCGCCAGCCGTTGTGCCGCATG
ATCCGTTCCACCGTGCGCCGCGCCACCGGGATGCCCTGCCGCTGCAGGTGCGCCCACATCTTCAGGCTGCCGTACAGGCTCTCCGGCGGGCGTTTCCCGT
CGGCGTCAGGTTCGTAGTAGCCGGCCAGGATCTCGGTGATCGTGGTGTCCCACAGGGCCCGTTTCGACGGCGCCGACGAGCGGTGGGCCCAGTAGGTGCG
CGGGGCGATCGCCACGCCCTGGATCCCCAGGGCGCGGCAGATCGGTACGACCCCGAACCGCTCCTTGTGTTCGTCGATGAACGCACAGATCAGCGGTGTA
GCGGGTCGCACTCCCGCGCGAAGAAAGTCGTTGCGGCCTTGAGGATTTCGATCGTCGCCTCAAGCTCCCTGGTCTTGCGGCGTAGCTCCCGCAGCTCCCG
GCTCTCCTCGCTGGATATCCCTGCCGCCTCACCGGCATCGACCTCGGCCTGGCGCACCCACTTGCGCAGCGTCTCCGTGTTCATCCCCAGCCGGCCCGCG
ATCGCCTTCATCGCCGCCCACTCTGAGTCGTAATCATCGCGGTGCTCGCGGACCAGCCGCACCGCCCTGGCCTTGGTGTTCTCGTCATACTTGCTCGGCA
TCGCGCCATCCTTCCCAACAAAGGAAGTGCGCATAAAACCGGGGACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 312 bp | 103 aa | 52 | 363 | + | No |
Description : First part of the transposase
ORF sequence :
MPSKYDENTKARAVRLVREHRDDYDSEWAAMKAIAGRLGMNTETLRKWVRQAEVDAGEAAGISSEESRELRELRRKTRELEATIEILKAATTFFARECDP
LHR
LHR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 294 | 1322 | + | No |
Description : Second part of the transposase
ORF sequence :
GDDRNPQGRNDFLRAGVRPATPLICAFIDEHKERFGVVPICRALGIQGVAIAPRTYWAHRSSAPSKRALWDTTITEILAGYYEPDADGKRPPESLYGSLK
MWAHLQRQGIPVARRTVERIMRHNGWRGVTRARRPPRTTERDPAAARAPDLVGRKWRVAAPNLLDVADFTYVPLDTGGFGYTAFVVDAYAGLIPGWDCSM
TKDAGFVERALRHAAVFRARQGHPFDATIHHSDAGSHYTAIHYGDTLMLEGLIPSIGTVGDALDNALCETTIGLYKTECVRPGSPFRVGPIRTLPDLEDM
TSAWVSWYNERRLMHRLGRIPPAEAEAEYYARLQAGKHTGHT
MWAHLQRQGIPVARRTVERIMRHNGWRGVTRARRPPRTTERDPAAARAPDLVGRKWRVAAPNLLDVADFTYVPLDTGGFGYTAFVVDAYAGLIPGWDCSM
TKDAGFVERALRHAAVFRARQGHPFDATIHHSDAGSHYTAIHYGDTLMLEGLIPSIGTVGDALDNALCETTIGLYKTECVRPGSPFRVGPIRTLPDLEDM
TSAWVSWYNERRLMHRLGRIPPAEAEAEYYARLQAGKHTGHT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1271 bp | 423 aa | 52 | 1322 | + | Yes |
Chemistry : DDE
ORF sequence :
MPSKYDENTKARAVRLVREHRDDYDSEWAAMKAIAGRLGMNTETLRKWVRQAEVDAGEAAGISSEESRELRELRRKTRELEATIEILKAATTFLRAGVRP
ATPLICAFIDEHKERFGVVPICRALGIQGVAIAPRTYWAHRSSAPSKRALWDTTITEILAGYYEPDADGKRPPESLYGSLKMWAHLQRQGIPVARRTVER
IMRHNGWRGVTRARRPPRTTERDPAAARAPDLVGRKWRVAAPNLLDVADFTYVPLDTGGFGYTAFVVDAYAGLIPGWDCSMTKDAGFVERALRHAAVFRA
RQGHPFDATIHHSDAGSHYTAIHYGDTLMLEGLIPSIGTVGDALDNALCETTIGLYKTECVRPGSPFRVGPIRTLPDLEDMTSAWVSWYNERRLMHRLGR
IPPAEAEAEYYARLQAGKHTGHT
ATPLICAFIDEHKERFGVVPICRALGIQGVAIAPRTYWAHRSSAPSKRALWDTTITEILAGYYEPDADGKRPPESLYGSLKMWAHLQRQGIPVARRTVER
IMRHNGWRGVTRARRPPRTTERDPAAARAPDLVGRKWRVAAPNLLDVADFTYVPLDTGGFGYTAFVVDAYAGLIPGWDCSMTKDAGFVERALRHAAVFRA
RQGHPFDATIHHSDAGSHYTAIHYGDTLMLEGLIPSIGTVGDALDNALCETTIGLYKTECVRPGSPFRVGPIRTLPDLEDMTSAWVSWYNERRLMHRLGR
IPPAEAEAEYYARLQAGKHTGHT
Blast result :
Comments
ISMysp10 is 77% aa similar to ISMysp3, 90% aa identical to a transposase in Mycobacterium canettii (Genbank F0203510), and 84% to a transposase in Mycobacterium sp. MOTTY36Y (Genbank CP003491).
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Gauthier, D.T., Helenthal, A.M., Rhodes, M.W., Vogelbein, W.K., Kator, H.I. (2011) Dis. Aquat. Org. 95: 113-124