ISMyma9

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Mycobacterium marinum
Mycobacterium marinum 324-958
DNA section
IS Length : 2162 bp

Ends


IR Length : 44/51

IRL : TGTCGGTTCCGGTCGAAAAGGGAGCAGGTGATTCCGGTCGAAAAGTGAGC
IRR : TGTCGGTTCCGGTCGAATCCTGAGCAGTTTCTTCCGGTCGAAAAGTGAGC

Insertion site


Left flankDirect repeatRight flankDR Length
ACGCAGCGGCGCAAGGGCTGTGCGG5
TGGCGGCGTGCCGGGCTGATCCGAG5
CGGAGCGTTTCGCTGTGCGGTTGAC5
GACCGTCGAAGGTGCTATAAATCCA5
CAGAATCGAGCCGATATGTCGTCAGCC7

DNA sequence

TGTCGGTTCCGGTCGAAAAGGGAGCAGGTGATTCCGGTCGAAAAGTGAGCACCCTTCGATTGGAGAGTGATCACTGTGGAGGATTGGGCCGAGATTCGTC
GGTTGTATCGGTCGGAGAAGTTGTCGCAGGCTGCGATCGCCCGACGGCTGGGTTTGTCGCGGAACACCGTGGCCAAGGCGCTGGGTTCGGATGGCCCGCC
GCGTTATGAGCGGGCGCCGGTGACGACCTCGGCGTGGGCGCAGGTCGAGCCAGCGGTGCGGGCGGTGCTTAGCCAGTATCCGAGGATGCCGGCGACGGTG
ATCGCCGAGCGGGTGGGCTGGATGGGTGGGCATTCGTGGTTTGGGGAAAACGTCGCACGAATCCGCCCGGAGTATGCCCCTGCTGATCCGTGCGACCGGC
TGGTGCATTTGCCCGGTGAGCAAGTGCAGTGCGATTTGTGGTTGCCCGGCCGGGTAGTGCCCGACCATGCCGGAGTGCTGCGGTCGTTTCCGGTGTTGGT
GATGGTGGCTGCCTACTCGCGGTTTGTCGCCGCGATGATGATCCCGTCGCGGGTCACCGGGGATCTGCTGGCCGGCATGTGGCAGCTGCTCTCTGGGTAC
ATCGGCGGGGTGCCTCGAACCCTGTTGTGGGACAACGAGGCCGGTATCGGTCAACGTGGCCGGCTGGCCGAAGGGGTGGCCGGGTTCTGCGGAGTACTGG
GCACCCGGCTTGTTCAGGCCCGACCCTATGATCCTGAAACCAAGGGGCTAGTCGAGCGAGCCAACGGGTATTTGCAGACGTCGTTTCTGCCGGGCCGGCG
CTTTTGCTCGGCTGCAGATTTCAACACCCAGCTCGCGCAGTGGCTGGCGCAGGTGGCCAACCCCCGCCGGCACGCCACGACCAAGACAATTCCCGCTCAG
GCCCTGGGCGCTGATTTGCAGGTGATGGCTGCGCTGCCGCCGGTGGCCCCGACCACGGGCACCACACTGACCACCCGGTTGGGTCGGGACTACTACATCA
GCATGGGCGGCAACGCCTATTCGGTGCATCCTGAGGTGATTGGTCGGATGATCACCGCGACGGTATCGCTGGATCGGGTGATGGCCCGCTGTGGTGAGCG
TGTCGTCGCTGACCACGAACGGCTTTGGGGTAGTGCGGGACTGGCTTGCGATCCTGAGCATCTGGCCGCGGCGGCGATCATGCGCGAGAACTTCCGGGAA
CGCTCGGGCGTCGGCGCTCACCTGGACGTGGAGGTCCAAGTCGCTGACCTGGGCGCCTATGACGCGGTGTTTGGGACCGGGGAGGTCGCCTGATGGCCAC
CAAACGCGCCCCCGCCCCCGGTGAGGCCGACAAGCTGATCGCCCACCAATCCCGGCTGCTCAAAGCCCCGCGGATCGCCGCCCACTACCATCGACTGGCC
GAACAAGGCCGCGCCGCGGGCTGGTCGCTGGAGGACTACCTGGCCGCCGTCTTGGCCGTGGAATCCAACGCCCGCGCCGAATCGGGGGCCCGCCAACGCA
TCCGCTATGCCGGGTTCCCGGCGATCAAGACGATCACCGACTTCGACTTCACCGCCCAACCCCACCTTGACCGAGCCCAGATCGCCCGCCTGGAAGCCGG
CGGCTGGCTGGCCGAAGCCCGCAACATCGTGCTGCTGGGCCCCCCGGGCACCGGCAAAACACATTTGGCGACCGCGCTGGCGATCGCAGCCGCCCAAGCC
GGACACCGGGTCGCGTTCGCCCCAGCCACCGGGTGGATCACCCGCCTGGCCGAAGCCCACCGCATCGGCAGCCTGGATGGCGAACTGCGCAAAATCTCGC
GCATCGGGCTCATCGTCATCGACGAGGTTGGATACATCCCCTTTGACACCGAAGCGGCCAACCTGTTCTTCCAACTGGTGTCGACCCGATACGAGAAATC
GTCGATCATCTTGACCTCCAACCTGCCGTTTTCCCGGTGGGGCCAGGTCTTCGGCGAAGCCACCATCGCCTCGGCGATGATCGACCGCATCGTGCACCAC
GCCGACGTCATCGCCCTCAAAGGCGCCAGCTACCGCATCAAACACACCGCAATCGAGTCCCTGCCCTCCGTCGAAGCCGACCGTCAGGCAGACTCAACCC
CGTAACACAACTGCTCACTTTTCGACCGGAAGAAACTGCTCAGGATTCGACCGGAACCGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1218 bp405 aa761293+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VEDWAEIRRLYRSEKLSQAAIARRLGLSRNTVAKALGSDGPPRYERAPVTTSAWAQVEPAVRAVLSQYPRMPATVIAERVGWMGGHSWFGENVARIRPEY
APADPCDRLVHLPGEQVQCDLWLPGRVVPDHAGVLRSFPVLVMVAAYSRFVAAMMIPSRVTGDLLAGMWQLLSGYIGGVPRTLLWDNEAGIGQRGRLAEG
VAGFCGVLGTRLVQARPYDPETKGLVERANGYLQTSFLPGRRFCSAADFNTQLAQWLAQVANPRRHATTKTIPAQALGADLQVMAALPPVAPTTGTTLTT
RLGRDYYISMGGNAYSVHPEVIGRMITATVSLDRVMARCGERVVADHERLWGSAGLACDPEHLAAAAIMRENFRERSGVGAHLDVEVQVADLGAYDAVFG
TGEVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
813 bp270 aa12932105+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MATKRAPAPGEADKLIAHQSRLLKAPRIAAHYHRLAEQGRAAGWSLEDYLAAVLAVESNARAESGARQRIRYAGFPAIKTITDFDFTAQPHLDRAQIARL
EAGGWLAEARNIVLLGPPGTGKTHLATALAIAAAQAGHRVAFAPATGWITRLAEAHRIGSLDGELRKISRIGLIVIDEVGYIPFDTEAANLFFQLVSTRY
EKSSIILTSNLPFSRWGQVFGEATIASAMIDRIVHHADVIALKGASYRIKHTAIESLPSVEADRQADSTP

 

Blast result :
Comments
ISMyma9 has 81% pairwise identity to an integrase in Mycobacterium chubuense (Genbank CP003054) and an 81.5% pairwise identity to a transposase in Mycobacterium abcessus subspecies bolletii (CP004375).
The ORF1 of ISMyma9 has a 78.6% pairwise identity to a Mycobacterium abscessus subspecies bolletii integrase (CP004375).
ORF2 of ISMyma9 has an 85.4% pairwise identity to an IstB domain protein in Mycobacterium abscessus (CP004375).
ISMyma9 displays characteristics of insertion sequences in the IS21 family due to its 50bp inverted repeats; 5-8bp direct repeats; two consecutive open reading frames; and terminating with 5'-CA-3' (Mahillon & Chandler, 1998; Xu et. al. 1993).
References
1] Gauthier, D.T., Helenthal, A.M., Rhodes, M.W., Vogelbein, W.K., Kator, H.I. (2011) Dis. Aquat. Org. 95: 113-124