ISBj11
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| BA000040 | ND | Bradyrhizobium japonicum | Bradyrhizobium japonicum USDA 110 DNA
Bradyrhizobium japonicum symbiotic gene region |
DNA section
IS Length : 2649 bp
Ends
IR Length : 18/26
IRL : TGCCGCGCGGCGAACAGGATGGAAGTGGAGCGTCGACCATGATGAGAGGG
IRR : TATAGCGCGACGATCTGGGTCGGAAGAGCGCGGCGATCAGGATGGCGAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAATCACACG | AATCGGA | GGGGCTATGA | 7 |
| CCCGCGCGTC | CGGCCC | GGCTGCCTCG | 6 |
| TCCGCATAAT | CGCGAC | CACGTTCGCG | 6 |
| CCCGCTCGCG | GTGGCC | AGTGACAAGC | 6 |
DNA sequence
TGCCGCGCGGCGAACAGGATGGAAGTGGAGCGTCGACCATGATGAGAGGGCTACGCCGGGCTCGTGACGCAGGGAGGGCGTAGCCCGACCGGAGTTACGA
GCCCGGCGTCGGCGCGATCCACAGCGGACCGCGCCGACTGGTGATCGCGGCCGGCTGGTTATGCAAGTGGTTCTTCCGCCAAGAGGAATCACTCGCGTGC
CAGGCCGACACATTACCGATCACCAAATGAGGCTCTACATGAAGTACCGTCAGACCGATAGCCCACCCGTGGCCGCCGCCAAGGCTTCGTTCAGCACCTC
GACCGCTTACCGGATCGAGAAGGATCGACGCCTTCCGTCGCAGAAGAAGGCTCCCCGCGGCCGTCGCCGGCCAGATCCCTTGGCCCGCGTATTTGAGACA
GAGATCGCGCCGATGCTGAAGGCCGCCCCCGGTGTGCGGCCGGTCACGATCTTCGAGGAGTTGCTCCGACGCCATCCCGAGCTCGGCGCCGGCATCCGTC
GCACGCTGGAGCGCCGGATCCGGGCCTGGCGGGCGATCCACGGCGAGGAGCAGGAGGTCATCTTCCGCCAGACCCACGAACCCGGTCAGCGCGGCCTGTC
CGACTTCACCGACATGGGCGAATTGGGTGTCACGATCGCGGGCGTACCGCTCGACCATCGTCTCTATCACTTCCGGCTGGCCTATTCCGGGTTTGAGCAC
GCCCATGTCGTGCTCGGCGGTGAGAGCTTCGTCGCTCTGGCCGAAGGCCTGCAGAATGCCTTGTGGTCACTCGGTGGGGCGCCACGGGAGCATCGCACCG
ACAGCCTGTCGGCCGCCTTTTGCAATCTCGACCGCGACGCCAAAGACGATCTGACGCGGCGATACGAAGACCTCTGTGCCCATTACGGCATGCGGCCTTC
CCGCAACAATCGTGGCATCGCCCACGAGAACGGGGCGATCGAGAGTTCGCATGGTCATCTCAAGCGAGCGATCGGCGACGCGCTGTTGCTGCGTGGCACC
GCCGACTTCGACGATCTAGCTGCCTATCGTGGCTTCATCGATGAGATCGCCAGCCGCCGCAATGCCCGCAACGCCAAGCGGATCGACAGTGAACGTAGCG
CACTTCAGGATCTGCCGGACCGCCGCACGTCGGACTATGAAGAGGTGATCGTCCACGTGACGTCGTCCGGCGGCTTCACCTTGCGCAAGGTGTTCTACAC
GGTGCCGTCGCGCTTGATCGGCCATCGGCTGCGGGTGCGCCTGTATGACGATCACCTCGACGTGTTTGTCGGCGGCACGCATCTCCTCACCTTGCCGCGC
GGGCGGCCGCATCCCAATGGCAAGCACGATCAGGTCGTCGATTATCGGCACGTGATCCATTCCTTGCGGCGCAAGCCGATGGCGCTCCTCAACCTGGTCT
ACCGCGACCAGCTGTTCCCCCGGGAAGCTTACCGCCGAGCCTTCGACGTCTTGCGCAAACGCTTACCGGACAAGAAGGCCTGCCGGATCATGGTCGATCT
CCTCGCACTCGCCCATGAGCGCGGTTGCGAGGCCGAACTCGCCAATCAGCTCACGGCTGACCTGAACGACGGCCGGCTGCCCGACCTCAACCGGCTACGT
ACTCACTTCGCCCCGGATCCCGCCCAGGTGCCGAACGTCGTGGTACGCCTCGCACCGCTCGCCACCTATGAATGCCTCATCGGTACCGCCGAGATCGGAG
GCGCCGCATGAGCACAACCAACGTAGTCGACACCGCGCGCCTCAATCTGTTGCTCAACGAGCTGCGGCTGCCCGCCATCAAGGCGCTGTGGCCGCAATTT
GCCGAGCAATCCGATAAAGAAGGCTGGCCGGCGGCGCGCTTCCTCGCCACCATTGCCGAGCACGAGATCGCTGAGCGCGGCCGCCGCCGCATCGAGCGCC
ATCTCGTCGAGGCGCGGCTGCCTACCGGAAAGACCTTTGACAGCTTCGACTTCGAGGCCGTGCCGATGATCTCCAAGGCGCAAATGACCGCACTCGCCGC
CGGCGACGGCTGGCTCGGCAAGGGCGCCAATCTGCTGCTGTTTGGTCCGCCCGGTGGAGGCAAGAGCCACTTGGCGGCAGCAATCGGCTTGGCCCTCATC
GAGAACGGATGGCGCGTCCTGTTCACCCGCACCACCGATCTCGTGCAGAAGCTCCAGGTGGCTCGCCGCGAGCTCAACCTCGAGGGCGCCATCAACCGCC
TCGATCGCTTCGATCTCGTCATCTTGGACGATCTTGCCTATGTCACCAAGGACCAGGCCGAGACCAGTGTGCTGTTCGAGCTCATCAGCGCACGCTACGA
GCGACGCTCTTTGCTGATCACCGCCAATCAGCCCTTTGGAGAATGGAACAAGGTCTTTCCGGACCCAGCTATGACCCTCGCGGCGATCGATCGCCTTGTT
CACCACGCCACCATCGTCGAGATGAACGTCGAGAGCTATCGCAGGCGGACTGCCCTCGAGCGAAAGCGTGGTCCAGGGCGGCCACCGGAGCACGCGACAC
AAAAAACGCTCGCTTGATTGACGCTCCGCGACAATCAAAGCAAACAAAACTCTTGCGCGCGACAATCATCGCGGCGATCATCATCGCGCCGCGACACTGA
CTCGCCATCCTGATCGCCGCGCTCTTCCGACCCAGATCGTCGCGCTATA
GCCCGGCGTCGGCGCGATCCACAGCGGACCGCGCCGACTGGTGATCGCGGCCGGCTGGTTATGCAAGTGGTTCTTCCGCCAAGAGGAATCACTCGCGTGC
CAGGCCGACACATTACCGATCACCAAATGAGGCTCTACATGAAGTACCGTCAGACCGATAGCCCACCCGTGGCCGCCGCCAAGGCTTCGTTCAGCACCTC
GACCGCTTACCGGATCGAGAAGGATCGACGCCTTCCGTCGCAGAAGAAGGCTCCCCGCGGCCGTCGCCGGCCAGATCCCTTGGCCCGCGTATTTGAGACA
GAGATCGCGCCGATGCTGAAGGCCGCCCCCGGTGTGCGGCCGGTCACGATCTTCGAGGAGTTGCTCCGACGCCATCCCGAGCTCGGCGCCGGCATCCGTC
GCACGCTGGAGCGCCGGATCCGGGCCTGGCGGGCGATCCACGGCGAGGAGCAGGAGGTCATCTTCCGCCAGACCCACGAACCCGGTCAGCGCGGCCTGTC
CGACTTCACCGACATGGGCGAATTGGGTGTCACGATCGCGGGCGTACCGCTCGACCATCGTCTCTATCACTTCCGGCTGGCCTATTCCGGGTTTGAGCAC
GCCCATGTCGTGCTCGGCGGTGAGAGCTTCGTCGCTCTGGCCGAAGGCCTGCAGAATGCCTTGTGGTCACTCGGTGGGGCGCCACGGGAGCATCGCACCG
ACAGCCTGTCGGCCGCCTTTTGCAATCTCGACCGCGACGCCAAAGACGATCTGACGCGGCGATACGAAGACCTCTGTGCCCATTACGGCATGCGGCCTTC
CCGCAACAATCGTGGCATCGCCCACGAGAACGGGGCGATCGAGAGTTCGCATGGTCATCTCAAGCGAGCGATCGGCGACGCGCTGTTGCTGCGTGGCACC
GCCGACTTCGACGATCTAGCTGCCTATCGTGGCTTCATCGATGAGATCGCCAGCCGCCGCAATGCCCGCAACGCCAAGCGGATCGACAGTGAACGTAGCG
CACTTCAGGATCTGCCGGACCGCCGCACGTCGGACTATGAAGAGGTGATCGTCCACGTGACGTCGTCCGGCGGCTTCACCTTGCGCAAGGTGTTCTACAC
GGTGCCGTCGCGCTTGATCGGCCATCGGCTGCGGGTGCGCCTGTATGACGATCACCTCGACGTGTTTGTCGGCGGCACGCATCTCCTCACCTTGCCGCGC
GGGCGGCCGCATCCCAATGGCAAGCACGATCAGGTCGTCGATTATCGGCACGTGATCCATTCCTTGCGGCGCAAGCCGATGGCGCTCCTCAACCTGGTCT
ACCGCGACCAGCTGTTCCCCCGGGAAGCTTACCGCCGAGCCTTCGACGTCTTGCGCAAACGCTTACCGGACAAGAAGGCCTGCCGGATCATGGTCGATCT
CCTCGCACTCGCCCATGAGCGCGGTTGCGAGGCCGAACTCGCCAATCAGCTCACGGCTGACCTGAACGACGGCCGGCTGCCCGACCTCAACCGGCTACGT
ACTCACTTCGCCCCGGATCCCGCCCAGGTGCCGAACGTCGTGGTACGCCTCGCACCGCTCGCCACCTATGAATGCCTCATCGGTACCGCCGAGATCGGAG
GCGCCGCATGAGCACAACCAACGTAGTCGACACCGCGCGCCTCAATCTGTTGCTCAACGAGCTGCGGCTGCCCGCCATCAAGGCGCTGTGGCCGCAATTT
GCCGAGCAATCCGATAAAGAAGGCTGGCCGGCGGCGCGCTTCCTCGCCACCATTGCCGAGCACGAGATCGCTGAGCGCGGCCGCCGCCGCATCGAGCGCC
ATCTCGTCGAGGCGCGGCTGCCTACCGGAAAGACCTTTGACAGCTTCGACTTCGAGGCCGTGCCGATGATCTCCAAGGCGCAAATGACCGCACTCGCCGC
CGGCGACGGCTGGCTCGGCAAGGGCGCCAATCTGCTGCTGTTTGGTCCGCCCGGTGGAGGCAAGAGCCACTTGGCGGCAGCAATCGGCTTGGCCCTCATC
GAGAACGGATGGCGCGTCCTGTTCACCCGCACCACCGATCTCGTGCAGAAGCTCCAGGTGGCTCGCCGCGAGCTCAACCTCGAGGGCGCCATCAACCGCC
TCGATCGCTTCGATCTCGTCATCTTGGACGATCTTGCCTATGTCACCAAGGACCAGGCCGAGACCAGTGTGCTGTTCGAGCTCATCAGCGCACGCTACGA
GCGACGCTCTTTGCTGATCACCGCCAATCAGCCCTTTGGAGAATGGAACAAGGTCTTTCCGGACCCAGCTATGACCCTCGCGGCGATCGATCGCCTTGTT
CACCACGCCACCATCGTCGAGATGAACGTCGAGAGCTATCGCAGGCGGACTGCCCTCGAGCGAAAGCGTGGTCCAGGGCGGCCACCGGAGCACGCGACAC
AAAAAACGCTCGCTTGATTGACGCTCCGCGACAATCAAAGCAAACAAAACTCTTGCGCGCGACAATCATCGCGGCGATCATCATCGCGCCGCGACACTGA
CTCGCCATCCTGATCGCCGCGCTCTTCCGACCCAGATCGTCGCGCTATA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1551 bp | 516 aa | 161 | 1711 | + | No |
Description :
ORF sequence :
MQVVLPPRGITRVPGRHITDHQMRLYMKYRQTDSPPVAAAKASFSTSTAYRIEKDRRLPSQKKAPRGRRRPDPLARVFETEIAPMLKAAPGVRPVTIFEE
LLRRHPELGAGIRRTLERRIRAWRAIHGEEQEVIFRQTHEPGQRGLSDFTDMGELGVTIAGVPLDHRLYHFRLAYSGFEHAHVVLGGESFVALAEGLQNA
LWSLGGAPREHRTDSLSAAFCNLDRDAKDDLTRRYEDLCAHYGMRPSRNNRGIAHENGAIESSHGHLKRAIGDALLLRGTADFDDLAAYRGFIDEIASRR
NARNAKRIDSERSALQDLPDRRTSDYEEVIVHVTSSGGFTLRKVFYTVPSRLIGHRLRVRLYDDHLDVFVGGTHLLTLPRGRPHPNGKHDQVVDYRHVIH
SLRRKPMALLNLVYRDQLFPREAYRRAFDVLRKRLPDKKACRIMVDLLALAHERGCEAELANQLTADLNDGRLPDLNRLRTHFAPDPAQVPNVVVRLAPL
ATYECLIGTAEIGGAA
LLRRHPELGAGIRRTLERRIRAWRAIHGEEQEVIFRQTHEPGQRGLSDFTDMGELGVTIAGVPLDHRLYHFRLAYSGFEHAHVVLGGESFVALAEGLQNA
LWSLGGAPREHRTDSLSAAFCNLDRDAKDDLTRRYEDLCAHYGMRPSRNNRGIAHENGAIESSHGHLKRAIGDALLLRGTADFDDLAAYRGFIDEIASRR
NARNAKRIDSERSALQDLPDRRTSDYEEVIVHVTSSGGFTLRKVFYTVPSRLIGHRLRVRLYDDHLDVFVGGTHLLTLPRGRPHPNGKHDQVVDYRHVIH
SLRRKPMALLNLVYRDQLFPREAYRRAFDVLRKRLPDKKACRIMVDLLALAHERGCEAELANQLTADLNDGRLPDLNRLRTHFAPDPAQVPNVVVRLAPL
ATYECLIGTAEIGGAA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 846 bp | 281 aa | 1672 | 2517 | + | No |
Description :
ORF sequence :
MPHRYRRDRRRRMSTTNVVDTARLNLLLNELRLPAIKALWPQFAEQSDKEGWPAARFLATIAEHEIAERGRRRIERHLVEARLPTGKTFDSFDFEAVPMI
SKAQMTALAAGDGWLGKGANLLLFGPPGGGKSHLAAAIGLALIENGWRVLFTRTTDLVQKLQVARRELNLEGAINRLDRFDLVILDDLAYVTKDQAETSV
LFELISARYERRSLLITANQPFGEWNKVFPDPAMTLAAIDRLVHHATIVEMNVESYRRRTALERKRGPGRPPEHATQKTLA
SKAQMTALAAGDGWLGKGANLLLFGPPGGGKSHLAAAIGLALIENGWRVLFTRTTDLVQKLQVARRELNLEGAINRLDRFDLVILDDLAYVTKDQAETSV
LFELISARYERRSLLITANQPFGEWNKVFPDPAMTLAAIDRLVHHATIVEMNVESYRRRTALERKRGPGRPPEHATQKTLA
Blast result :
Comments
ISBj11 is 84%(ORFA, the transposase) and 88% (ORFB, helper of transposition) aa similar to ISRel5.
References
1] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 189-197.
2] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256.
2] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256.