ISBj2
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| BA000040 | ND | Bradyrhizobium japonicum | Bradyrhizobium japonicum USDA 110 |
DNA section
IS Length : 830 bp
Ends
IR Length : 15/22
IRL : GGTCTGTACCTAAATAGCGCCACGTGATTCTCTTGCCTACGTGTTGATTC
IRR : GGTCCAGACTCAATTGAGCCAATATGCGACGAGAGCGGCGAGATGAACAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAACCTCAC | CTAG | CACTACTTTG | 4 |
DNA sequence
GGTCTGTACCTAAATAGCGCCACGTGATTCTCTTGCCTACGTGTTGATTCGGGGGCGAGAGAATGCGCGCTGGTTTTTTTGGCTGAACGACAGGCAATGG
GCGCGTATCGAACCGCATCTGCCGAGGGGACTGACGGGGCCGGATCGGGACGACGACCGACGCATCGTCAGCGGCATCATTCACATGCTGCAATCGGGTG
CACGATGGCGTGATTGTCCACGTGAATACGGCCCTTACACGACGATCTACAATCGCTTCAATCGCTGGGCCAAGCGAGGACGATGGTGCGCAATCTTCGA
AGCGCTGGCCAAGCCTGGCGAAGACGGCGTCGTACTGTCGCTCGACTCGACCTCGATTAAAGCTCACCGGTGTGCCTCCGGCGGAAAAGGGGAAGCACAA
TCAAGCAATCGGCCGCTCGCGCGGAGGCCGCACGACAAAAATCCATGCGCTGAGCGATCCGCTCTGCCGGCCGGTCGTCCTGCATCTGACTCCAGGCCAG
GATGCCGATATCGCTGCGGCTCCCGATGTCCTGGCGCTCGCGCCACCCATGAGCGTGCTCCTCGCCGACAAAGGGTATGATGGCGACAAGCTTCGCGGCG
CAATCATTCGTCGTGGCGCCAAGCCCGTAATCCCCAATAAATCTAACCGTGTCGTCATCCATCGCTTCAACAAACGCGCCTACAAAGGACGAAATGTCAT
CGAACGCTGCTTTTGCAGGCTCAAGGACTTCCGGCGCATCGCCATGCGATATGACAAGCTCGCCCGTAATTTTTTGGCCGCTGTTCATCTCGCCGCTCTC
GTCGCATATTGGCTCAATTGAGTCTGGACC
GCGCGTATCGAACCGCATCTGCCGAGGGGACTGACGGGGCCGGATCGGGACGACGACCGACGCATCGTCAGCGGCATCATTCACATGCTGCAATCGGGTG
CACGATGGCGTGATTGTCCACGTGAATACGGCCCTTACACGACGATCTACAATCGCTTCAATCGCTGGGCCAAGCGAGGACGATGGTGCGCAATCTTCGA
AGCGCTGGCCAAGCCTGGCGAAGACGGCGTCGTACTGTCGCTCGACTCGACCTCGATTAAAGCTCACCGGTGTGCCTCCGGCGGAAAAGGGGAAGCACAA
TCAAGCAATCGGCCGCTCGCGCGGAGGCCGCACGACAAAAATCCATGCGCTGAGCGATCCGCTCTGCCGGCCGGTCGTCCTGCATCTGACTCCAGGCCAG
GATGCCGATATCGCTGCGGCTCCCGATGTCCTGGCGCTCGCGCCACCCATGAGCGTGCTCCTCGCCGACAAAGGGTATGATGGCGACAAGCTTCGCGGCG
CAATCATTCGTCGTGGCGCCAAGCCCGTAATCCCCAATAAATCTAACCGTGTCGTCATCCATCGCTTCAACAAACGCGCCTACAAAGGACGAAATGTCAT
CGAACGCTGCTTTTGCAGGCTCAAGGACTTCCGGCGCATCGCCATGCGATATGACAAGCTCGCCCGTAATTTTTTGGCCGCTGTTCATCTCGCCGCTCTC
GTCGCATATTGGCTCAATTGAGTCTGGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 540 bp | 179 aa | 41 | 580 | + | No |
Description : First part of the transposase
ORF sequence :
MLIRGRENARWFFWLNDRQWARIEPHLPRGLTGPDRDDDRRIVSGIIHMLQSGARWRDCPREYGPYTTIYNRFNRWAKRGRWCAIFEALAKPGEDGVVLS
LDSTSIKAHRCASGGKGEAQSSNRPLARRPHDKNPCAERSALPAGRPASDSRPGCRYRCGSRCPGARATHERAPRRQRV
LDSTSIKAHRCASGGKGEAQSSNRPLARRPHDKNPCAERSALPAGRPASDSRPGCRYRCGSRCPGARATHERAPRRQRV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 687 bp | 228 aa | 135 | 821 | + | No |
Description : Second part of the transposase
ORF sequence :
RGRIGTTTDASSAASFTCCNRVHDGVIVHVNTALTRRSTIASIAGPSEDDGAQSSKRWPSLAKTASYCRSTRPRLKLTGVPPAEKGKHNQAIGRSRGGRT
TKIHALSDPLCRPVVLHLTPGQDADIAAAPDVLALAPPMSVLLADKGYDGDKLRGAIIRRGAKPVIPNKSNRVVIHRFNKRAYKGRNVIERCFCRLKDFR
RIAMRYDKLARNFLAAVHLAALVAYWLN
TKIHALSDPLCRPVVLHLTPGQDADIAAAPDVLALAPPMSVLLADKGYDGDKLRGAIIRRGAKPVIPNKSNRVVIHRFNKRAYKGRNVIERCFCRLKDFR
RIAMRYDKLARNFLAAVHLAALVAYWLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 781 bp | 259 aa | 41 | 821 | + | Yes |
Chemistry : DDE
ORF sequence :
MLIRGRENARWFFWLNDRQWARIEPHLPRGLTGPDRDDDRRIVSGIIHMLQSGARWRDCPREYGPYTTIYNRFNRWAKRGRWCAIFEALAKPGEDGVVLS
LDSTSIKAHRCASGGKGKHNQAIGRSRGGRTTKIHALSDPLCRPVVLHLTPGQDADIAAAPDVLALAPPMSVLLADKGYDGDKLRGAIIRRGAKPVIPNK
SNRVVIHRFNKRAYKGRNVIERCFCRLKDFRRIAMRYDKLARNFLAAVHLAALVAYWLN
LDSTSIKAHRCASGGKGKHNQAIGRSRGGRTTKIHALSDPLCRPVVLHLTPGQDADIAAAPDVLALAPPMSVLLADKGYDGDKLRGAIIRRGAKPVIPNK
SNRVVIHRFNKRAYKGRNVIERCFCRLKDFRRIAMRYDKLARNFLAAVHLAALVAYWLN
Blast result :
Comments
ISBj2 is 75% aa similar to ISMex15.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References
1] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 189-197.
2] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256.
2] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256.