ISFK1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AP012206 ND Bradyrhizobium japonicum
Bradyrhizobium japonicum USDA 6
Bradyrhizobium japonicum NK5
DNA section
IS Length : 2592 bp

Ends


IR Length : 38/48

IRL : TGCGCGTTTCGCGGGATCGTGAGCGCGGATTTCAGGGGATCGTGAGCAGA
IRR : TGCGGATTTCACGGATCGTGAGCATCGATTTCGCGCGATCGTGAGCACTG

Insertion site


Left flankDirect repeatRight flankDR Length
CCGATGTGCTGCTGGACGCGAAGATCGC8

DNA sequence

TGCGCGTTTCGCGGGATCGTGAGCGCGGATTTCAGGGGATCGTGAGCAGAGATTTCAGACGATCGTGAGCAACGATTTCGCGGGATCGTGAGCAAGGCTT
TCGGAGCCTTGCAGCGCTTCGGCCGACAACTCAACCGGCTTAGGGATGACCTCGTGGTTAACGAGGAAGCCCAATGCCTACCCAGAGATTGTCGATGCGC
CGGATCAAGGAAGTCCTTCGGTTAGAACATTTTCAAGGCCTGCCAGAGCGGGCCATCGCGCGGAGCGTGGGCGTCAGCAACGGCGTTGTGCACAGCTACC
TGAGCCGCGCCCGCTCTGCTGGGTTGAGCTGGCCGCTTCCGGAGGGAATGACCGATGAAGACCTGGAGCTTTTGCTTTTCCCAGCCCCACGACCAGCGTC
TCAGAGCCCGCAGCGGCCGGTGCCCGACTGGAGCTACATCGATAAGGAGCTCCGCCGGCGCAACGTAACCCGTCGCCTGCTCTGGGAGGAGTATCGCGCC
GTTAATCCCGACGGTTTCGGGTACACGTGGTTCTGCACTACCTACGAGGCCTGGAAGGGGCGGGTCCGACCTTCGATGCGGCAGATTCATCTGGGCGGCG
AGAAGGTGTTCGTGGATTTCGCCGGCGACACCATCGACATCGTCGATCCCCTGACCGGGGAAGTGCAGCCGATGAAGCTGTTCGTCGCGGCGATGGGCGC
TTCGAACTACACCTACGCCGAGGCCTGCCCCAGCGAGAGCTTGGCCGACTGGATCCGGGCCCACGTCAACTTGTTCACGTTTTTGAGCGGTGCGCCGACG
TTCGTGGTCTGCGACAACCTCAAAGCCGCCGTCAGCAACCCCGACCGCTACGATCCCGGCCTCAATCGCACTTATGCCGAGATGGCGAGCCATTACGGCA
CGGCCATTCTCGCCGCACGGCCGCGGCGCCCAAAAGACAAGGCGAAGGTCGAGGTCGCGGTGCAAATCGCCCAGCGCTGGATTCTGGCCCGGCTGCGCAA
TCAGCGCTTCTTTTCCCGGGCCGAGCTCAACGCCGCCATCAAGACACTCGTCGACGAACTCAATGCTCGTCAAATGCGTGGCTTCGGCTCAAGCCGCGCC
GAACTGTTTGCCGAACTCGACAAACCCAAGCTAACCCCGCTGCCAGATCAGCCTTATGCCTTCGCACGCTGGAAGCGCTGCCGCCTCGCTCCCGATTATC
ATGTCGAGGTCGACGGCCATTGGTACTCCGCGCCGTATCGTCTGATTGGCGAGCTGGTCGATGCCCGTATCGACGATCGGACGGTCGAGATCTTCCACAA
GGGCCAGCGGATCGCCAGCCATGCCCGCGCGCCCAACCGACGCGGACACACCACCATCGCCGACCACATGCCCAGCGCCCATCGCCGCTACGGCGAATGG
ACCCCCGCCGCGGTGATCGCCGCCGGCGAGCGGATCGGTCCTTCGACAGCAGCGTTTTTCCAGGCCGTGATCGACGCCCGGCCCCATCCAGAACAAGGCT
TTCGAACCTGCCTTGGCATTCTGGCGCTCGTCAAAAGCTACGGCGCCGAACGCCTCGACGCAGCCTGCCGGAGGGGCATCCTCATCAAGGCGCGCTCCGT
CGCCTCGATTAGATCGATCCTCCAGAACGGCCTCGATCGCACGTTCTTCGACGAATCTTTCGAGCACCAGCCCCTGCGCCACGGCAACATCCGCGGACGC
GACTACTTCCACTGAAGCAAGGAGAGACCCGGCATGCTTAACCACCCAACCCACGAACGGCTGATCGAGCTTGGCCTGACCGGAATGGCCAAGGCCTTCG
AGGAGCAGCGCCGATCGCCCGATCTCGAAGCCCTGCCGTTCGAAGATCGCATCGGCCTGTTGGTCGACCGCGAAGCCGCCGAACGCGACACCAGGCGGCT
CACCACGCGCCTCAAGATCGCCGCACTGCGCCAGACTGCTTGCGTCGAGGACGTCGATCTGCGCACCCCGCGGGGCATCGACCGCGCCGTTTTCGCCAAA
CTCGTCGAAGGTCGCTGGATCGATCGCCACGAGAATTTGCTCGTCACCGGGGCAACCGGCCTGGGCAAAAGTTGGTTAGCCTGCGCGCTCGGCCACAAGG
CCTGCCGCGACAACCGATCAGTCCTCTATCATCGCGTTCCAAGGCTGTTCGAGGCGCTCGCGCTCGCGCGCGGAGACGGACGTTACGCCCGGCTCCTCAA
AAGCCTCGGCCGCGCTCAGCTTCTGATTTTGGATGATTGGGGACTATCGGTGCTCACCGCCGCGGAACGCCGCGATCTGCTCGAAATCCTCGAGGACCGA
CATGGCCGCGCATCCACCATCGTCACAAGTCAGCTCCCCGTGGACACCTGGCATGGAGCCATTGGGGACCCCACGGTCGCCGACGCCATTCTCGATCGCC
TCGTCCACAACGCCCACCGCCTCCAGCTCACCGGAGAAAGCATGCGAAAACGCAGCGCCAAAACCATCACCCTTGACGGCCAACCAGAACACTGACTCTA
TCTCCCATCGGCCGTAGCGGGCTGCTCACGATCGTCTGAATTCAGTGCTCACGATCGCGCGAAATCGATGCTCACGATCCGTGAAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1542 bp513 aa1741715+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTQRLSMRRIKEVLRLEHFQGLPERAIARSVGVSNGVVHSYLSRARSAGLSWPLPEGMTDEDLELLLFPAPRPASQSPQRPVPDWSYIDKELRRRNVTR
RLLWEEYRAVNPDGFGYTWFCTTYEAWKGRVRPSMRQIHLGGEKVFVDFAGDTIDIVDPLTGEVQPMKLFVAAMGASNYTYAEACPSESLADWIRAHVNL
FTFLSGAPTFVVCDNLKAAVSNPDRYDPGLNRTYAEMASHYGTAILAARPRRPKDKAKVEVAVQIAQRWILARLRNQRFFSRAELNAAIKTLVDELNARQ
MRGFGSSRAELFAELDKPKLTPLPDQPYAFARWKRCRLAPDYHVEVDGHWYSAPYRLIGELVDARIDDRTVEIFHKGQRIASHARAPNRRGHTTIADHMP
SAHRRYGEWTPAAVIAAGERIGPSTAAFFQAVIDARPHPEQGFRTCLGILALVKSYGAERLDAACRRGILIKARSVASIRSILQNGLDRTFFDESFEHQP
LRHGNIRGRDYFH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
762 bp253 aa17342495+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLNHPTHERLIELGLTGMAKAFEEQRRSPDLEALPFEDRIGLLVDREAAERDTRRLTTRLKIAALRQTACVEDVDLRTPRGIDRAVFAKLVEGRWIDRHE
NLLVTGATGLGKSWLACALGHKACRDNRSVLYHRVPRLFEALALARGDGRYARLLKSLGRAQLLILDDWGLSVLTAAERRDLLEILEDRHGRASTIVTSQ
LPVDTWHGAIGDPTVADAILDRLVHNAHRLQLTGESMRKRSAKTITLDGQPEH

 

Blast result :
Comments
ISFK1 is 66% (ORFA : the transposase) aa similar to ISSpu5 and 78% (ORFB : the helper of transposition) to ISIde2.
References
1] Isawa,T., Sameshima,R., Mitsui,H. and Minamisawa,K. (1999) Appl. Environ. Microbiol. 65 (8), 3493-3501
2] Kaneko,T., Maita,H., Hirakawa,H., Uchiike,N., Minamisawa,K., Watanabe,A. and Sato,S. (2011) Genes (Basel) 2 (4), 763-787.