ISLho1
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Lactobacillus hokkaidonensis | Lactobacillus hokkaidonensis LOOC260 |
DNA section
IS Length : 1547 bp
Ends
IR Length : 19/23
IRL : GTTAGTGTAAGGTTTTCCTAGATGGTCAACATATTTCTAGGAATATTCAT
IRR : GTATGTGTCAAGTTTTCCTAGATACCTTTTATTCGACTTAATATTTATAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAATTTAGGG | TTTTTGTTT | TGTGTTCTTG | 9 |
| CGCTTTATAG | TACACTATC | GAATATCTAC | 9 |
| TTCACGGCTG | CTCACACTC | CTATTCTTTG | 9 |
| TTAACGCAGA | GCCTAGCGT | GCGACGGTTG | 9 |
| GCTAATATTC | ATCTTTACC | TGCATATTAA | 9 |
| AATAACTGCT | CCCTACGCC | AAATAACTAT | 9 |
| AAATTAATTA | ATCTATTAG | GCTTTATTTG | 9 |
| TAATGGGAAA | ACGCCAACA | ACACGCATTG | 9 |
| TGTGTGCATA | TTTACAAA | TCAATAGTCG | 8 |
| TTTTTCAAAG | CATCAAAAG | CACTCTGTTG | 9 |
| TCAAACGCAA | ATGGATAA | TTAACACTAG | 8 |
| CTTACATATA | TTATGATT | TTAGTACTTG | 8 |
| GTATACATAC | AAAACAGAT | CATCTAGTTT | 9 |
DNA sequence
GTTAGTGTAAGGTTTTCCTAGATGGTCAACATATTTCTAGGAATATTCATTTTAGATTGAATATATAAGAAAAGACCTGTAGCCTTTTAATCGACGAAGA
TTAAAAGAAAGGATGCAGGTCTTTCATGTTTATTTTAGCAGATTTTATTGATTCATTGAATAATTTAGATAGTTTATTTGATTTGGAGGAACAAGTTATT
CGTTGTTTGCGGGAAATGTTTCAAGAAATTGTATCTAAATACTTAATTCAATTAGACGAAACGTTAGTTTCTCAGATTCCAAGTGACCATACCTTTATTA
ACCGACAACCACGAACAATTAATTTTATGTTTGGTGCTGTTTCATTTGAACGTAGATGTTATAGGAAGACAGATGGAACCAATTATTTTCCACTGGATAC
ACATTTAAAACTTGTGTCGCGAAAAAGGTTTTCACCATATTTTAAAAGTGTGGTTAGTAAGATTGGTCAAATGACTACCATGAGAAACACAGCGGATATG
ATTAACCTTGCCAGTCAGACTGATATTAGTGCATGGGCAGTCGACAAAATCGTTAGAGAGATGGCCGACATCGTTGCTGTCGAGGAAGAAACACTTGATA
AAAAAATTGTTCATCGTAAAAAAGTGGATAATTTAGTAATTGAAGGAGATGCTTTTGAAGCCCGAGAACGTGGTAAGCAACGGGTTTCTGTGCATCATTA
TAGGGTATACGAATCCACTAATGCTGGTCCAGTAAATAAGCGTGAATTTGTTGAAACCAATCATTTAAAAGCACGAAAACAAGTTTGTGATTATCTGGAA
GTACATTATAAATTAAGCGAAATGGTAGTGTTTTTAGCAAGTGATGCCGGTCCTGGATATGATCCCATCAGTATGCGCGAATTAGTTCCTGGGGCAAAAA
AAGTTGAGTATGTAATTGATCGGTATCACTTTATTCGAAAATTCGAGCAGACCATCGGTCTACAAAACCCGTTAAGTAGAAAGGCTACAGCCGCTATTAG
AGGACATAATTTGAACCAATTGGAGGCTATTTTAGATACTTTTGAATCGCAAATTACAACTGGAAAAGATTCCGAAAAGTTGATCAAATTAAGACATTAT
CTAAGCCGTAATTGGAAATATATCAAACGTCCCAAAGATCGCGGCTATAAATATATGGGCAAATTAGGCTCAGTTGAGAGCTCACACAGAGCTTTCACTT
ACCGCTTAAAAAAGCAGGGTAAGAGTTGGTCTAAAGAAGGATTACAAGCTATGCTAGTTCTCATACTAGCAAGAGTTAACAGACATCTTAATCAAGATCT
ATCATCAGGATTAAGAAGGCTAAGAGAACTTAAAATTGAAGTATCTCTCGAGCCAATTAAATCAATTAGGTTCACAGATTTAAACCGAAAAACACGTTCA
CATCACATAGGCGTTAAAATTGGTAATATTACTGTTGATTCATCAACGAGTAGCCCCATAGGGGCAATGGCAAAAGCATACTCCCGTTAATTCGGATGTA
TAAATATTAAGTCGAATAAAAGGTATCTAGGAAAACTTGACACATAC
TTAAAAGAAAGGATGCAGGTCTTTCATGTTTATTTTAGCAGATTTTATTGATTCATTGAATAATTTAGATAGTTTATTTGATTTGGAGGAACAAGTTATT
CGTTGTTTGCGGGAAATGTTTCAAGAAATTGTATCTAAATACTTAATTCAATTAGACGAAACGTTAGTTTCTCAGATTCCAAGTGACCATACCTTTATTA
ACCGACAACCACGAACAATTAATTTTATGTTTGGTGCTGTTTCATTTGAACGTAGATGTTATAGGAAGACAGATGGAACCAATTATTTTCCACTGGATAC
ACATTTAAAACTTGTGTCGCGAAAAAGGTTTTCACCATATTTTAAAAGTGTGGTTAGTAAGATTGGTCAAATGACTACCATGAGAAACACAGCGGATATG
ATTAACCTTGCCAGTCAGACTGATATTAGTGCATGGGCAGTCGACAAAATCGTTAGAGAGATGGCCGACATCGTTGCTGTCGAGGAAGAAACACTTGATA
AAAAAATTGTTCATCGTAAAAAAGTGGATAATTTAGTAATTGAAGGAGATGCTTTTGAAGCCCGAGAACGTGGTAAGCAACGGGTTTCTGTGCATCATTA
TAGGGTATACGAATCCACTAATGCTGGTCCAGTAAATAAGCGTGAATTTGTTGAAACCAATCATTTAAAAGCACGAAAACAAGTTTGTGATTATCTGGAA
GTACATTATAAATTAAGCGAAATGGTAGTGTTTTTAGCAAGTGATGCCGGTCCTGGATATGATCCCATCAGTATGCGCGAATTAGTTCCTGGGGCAAAAA
AAGTTGAGTATGTAATTGATCGGTATCACTTTATTCGAAAATTCGAGCAGACCATCGGTCTACAAAACCCGTTAAGTAGAAAGGCTACAGCCGCTATTAG
AGGACATAATTTGAACCAATTGGAGGCTATTTTAGATACTTTTGAATCGCAAATTACAACTGGAAAAGATTCCGAAAAGTTGATCAAATTAAGACATTAT
CTAAGCCGTAATTGGAAATATATCAAACGTCCCAAAGATCGCGGCTATAAATATATGGGCAAATTAGGCTCAGTTGAGAGCTCACACAGAGCTTTCACTT
ACCGCTTAAAAAAGCAGGGTAAGAGTTGGTCTAAAGAAGGATTACAAGCTATGCTAGTTCTCATACTAGCAAGAGTTAACAGACATCTTAATCAAGATCT
ATCATCAGGATTAAGAAGGCTAAGAGAACTTAAAATTGAAGTATCTCTCGAGCCAATTAAATCAATTAGGTTCACAGATTTAAACCGAAAAACACGTTCA
CATCACATAGGCGTTAAAATTGGTAATATTACTGTTGATTCATCAACGAGTAGCCCCATAGGGGCAATGGCAAAAGCATACTCCCGTTAATTCGGATGTA
TAAATATTAAGTCGAATAAAAGGTATCTAGGAAAACTTGACACATAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1365 bp | 454 aa | 126 | 1490 | + | No |
Chemistry : DDE
ORF sequence :
MFILADFIDSLNNLDSLFDLEEQVIRCLREMFQEIVSKYLIQLDETLVSQIPSDHTFINRQPRTINFMFGAVSFERRCYRKTDGTNYFPLDTHLKLVSRK
RFSPYFKSVVSKIGQMTTMRNTADMINLASQTDISAWAVDKIVREMADIVAVEEETLDKKIVHRKKVDNLVIEGDAFEARERGKQRVSVHHYRVYESTNA
GPVNKREFVETNHLKARKQVCDYLEVHYKLSEMVVFLASDAGPGYDPISMRELVPGAKKVEYVIDRYHFIRKFEQTIGLQNPLSRKATAAIRGHNLNQLE
AILDTFESQITTGKDSEKLIKLRHYLSRNWKYIKRPKDRGYKYMGKLGSVESSHRAFTYRLKKQGKSWSKEGLQAMLVLILARVNRHLNQDLSSGLRRLR
ELKIEVSLEPIKSIRFTDLNRKTRSHHIGVKIGNITVDSSTSSPIGAMAKAYSR
RFSPYFKSVVSKIGQMTTMRNTADMINLASQTDISAWAVDKIVREMADIVAVEEETLDKKIVHRKKVDNLVIEGDAFEARERGKQRVSVHHYRVYESTNA
GPVNKREFVETNHLKARKQVCDYLEVHYKLSEMVVFLASDAGPGYDPISMRELVPGAKKVEYVIDRYHFIRKFEQTIGLQNPLSRKATAAIRGHNLNQLE
AILDTFESQITTGKDSEKLIKLRHYLSRNWKYIKRPKDRGYKYMGKLGSVESSHRAFTYRLKKQGKSWSKEGLQAMLVLILARVNRHLNQDLSSGLRRLR
ELKIEVSLEPIKSIRFTDLNRKTRSHHIGVKIGNITVDSSTSSPIGAMAKAYSR
Blast result :
Comments
ISLho1 is 62% aa similarity with ISLre2.
13 copies were found in the chromosome of Lactobacillus hokkaidonensis LOOC260.
13 copies were found in the chromosome of Lactobacillus hokkaidonensis LOOC260.
References
1] Tanizawa Y, Tohono M, Kaminuma E, Nakamura Y, Arita M (2014) Genome sequencing and comparative genomics of psychrotrophic Lactobacillus hokkaidonensis LOOC260T (on preparation)