ISKpn28
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009649 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae CG43 pLVPK Klebsiella pneumoniae subsp. pneumoniae MGH 78578 plasmid pKPN3 Klebsiella pneumoniae KCTC 2242 |
DNA section
IS Length : 1096 bp
Ends
IR Length : 22/28
IRL : TGTGTAAGTTCACACAGATATTGCAATTGCCTCAGAACACTAAAGGAGGG
IRR : TGTATGGGTCCACCACATATTGCAATTTTTACTCTCACGTAAAATATAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGGTTTACT | CCTCC | TTGCAAATTG | 5 |
| ATGGTTTACT | CCTCC | TTGCAAATTG | 5 |
| CTTTTTCTCC | ATTCCAACGC | 0 | |
| ACGACACGCT | GTCGC | TTGCATGACC | 5 |
| AACGGCGGTC | AGCTC | CATACTTTTC | 5 |
| AGAATTGCAA | AGTCT | TTGAATTAAA | 5 |
| CGGCAATGGC | GGACG | TTGATTTAAA | 5 |
| TCCTCGCCTC | CAGTG | TTGATTTTCA | 5 |
DNA sequence
TGTGTAAGTTCACACAGATATTGCAATTGCCTCAGAACACTAAAGGAGGGATTGCCAGATGTTGATGCAGCATATCGGTGTCGGCTATTTTGGGTATTAC
CGAGCAACTGCTTATGCGATGAAACACTCTCTTATGCCCGAGATTGCGAAGTTAAGAATGAAGGCTCTGAACTTCTGGGATAAGCACGGGATCCGTGCCG
CAGCTGATGCTTTTGACGTATCAACGCGAACACTCTACTGGTGGCGCCGGTTACTGCGCACCGGTGGTCCAGAAGCACTAATTCCAAGAAGCAAAGCCCC
TCTGGTTCGCCGTTCAAGGCACTGGCATCCTGATGTACTCAAGGAGATCAGGCGTCTGAGAACTGAGTTACCCAATCTCGGCAAAGAGCAGATTTTTGTC
AGGCTGAAACCATGGTGTGAAGCGCGGCATTTTACCTGTCCCAGCACGTCAACCATTGGAAGAATCATTGCTGGTGCTCACGATAAAATGCGGATGATCC
CCGTACGCCTCAGCGCCAGGGGCAAAGCCCGGTTGATAAAAAAACGCTCAGTGAAGCCCAGAAGACCAAAACAATACCGCCCGGTAAAAACAGGCGAACT
CATAGGGATGGACGCGATTGAACTCAGGATGGGGGACCTACGCCGCTATATCATTACCATGATCGACGAGCACAGCGACTATGCGCTGGCCCTGGCGGTC
CCTTCACTCAACAGCGATATTACCAGCCATTTCTTCAGCAAGGCCACAAAGCTCTTTCCTGTCGCTATCAGACAAGTTGTCACTGACAACGGTAAGGAGT
TCCTCGGTAACTTTGATAAAACGCTACAGGAAGCCTCGATTAAACACATCTGGACCTATCCGTACACACCGAAAATGAATGCGACCTGTGAGCGATTTAA
CCGGACACTTAGAGAACAATTTATTGAATTCAATGAATTGTTGCTTTTTGAGGACCTGAATTTGTTTAATCAAAGAATGGCTGAATATCTGGTGCTGTAT
AACAGCAAAAGGCCACATAAATCACTCGAACTGATGACGCCAGTGGACTATATTTTACGTGAGAGTAAAAATTGCAATATGTGGTGGACCCATACA
CGAGCAACTGCTTATGCGATGAAACACTCTCTTATGCCCGAGATTGCGAAGTTAAGAATGAAGGCTCTGAACTTCTGGGATAAGCACGGGATCCGTGCCG
CAGCTGATGCTTTTGACGTATCAACGCGAACACTCTACTGGTGGCGCCGGTTACTGCGCACCGGTGGTCCAGAAGCACTAATTCCAAGAAGCAAAGCCCC
TCTGGTTCGCCGTTCAAGGCACTGGCATCCTGATGTACTCAAGGAGATCAGGCGTCTGAGAACTGAGTTACCCAATCTCGGCAAAGAGCAGATTTTTGTC
AGGCTGAAACCATGGTGTGAAGCGCGGCATTTTACCTGTCCCAGCACGTCAACCATTGGAAGAATCATTGCTGGTGCTCACGATAAAATGCGGATGATCC
CCGTACGCCTCAGCGCCAGGGGCAAAGCCCGGTTGATAAAAAAACGCTCAGTGAAGCCCAGAAGACCAAAACAATACCGCCCGGTAAAAACAGGCGAACT
CATAGGGATGGACGCGATTGAACTCAGGATGGGGGACCTACGCCGCTATATCATTACCATGATCGACGAGCACAGCGACTATGCGCTGGCCCTGGCGGTC
CCTTCACTCAACAGCGATATTACCAGCCATTTCTTCAGCAAGGCCACAAAGCTCTTTCCTGTCGCTATCAGACAAGTTGTCACTGACAACGGTAAGGAGT
TCCTCGGTAACTTTGATAAAACGCTACAGGAAGCCTCGATTAAACACATCTGGACCTATCCGTACACACCGAAAATGAATGCGACCTGTGAGCGATTTAA
CCGGACACTTAGAGAACAATTTATTGAATTCAATGAATTGTTGCTTTTTGAGGACCTGAATTTGTTTAATCAAAGAATGGCTGAATATCTGGTGCTGTAT
AACAGCAAAAGGCCACATAAATCACTCGAACTGATGACGCCAGTGGACTATATTTTACGTGAGAGTAAAAATTGCAATATGTGGTGGACCCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1038 bp | 345 aa | 59 | 1096 | + | No |
Chemistry : DDE
ORF sequence :
MLMQHIGVGYFGYYRATAYAMKHSLMPEIAKLRMKALNFWDKHGIRAAADAFDVSTRTLYWWRRLLRTGGPEALIPRSKAPLVRRSRHWHPDVLKEIRRL
RTELPNLGKEQIFVRLKPWCEARHFTCPSTSTIGRIIAGAHDKMRMIPVRLSARGKARLIKKRSVKPRRPKQYRPVKTGELIGMDAIELRMGDLRRYIIT
MIDEHSDYALALAVPSLNSDITSHFFSKATKLFPVAIRQVVTDNGKEFLGNFDKTLQEASIKHIWTYPYTPKMNATCERFNRTLREQFIEFNELLLFEDL
NLFNQRMAEYLVLYNSKRPHKSLELMTPVDYILRESKNCNMWWTH
RTELPNLGKEQIFVRLKPWCEARHFTCPSTSTIGRIIAGAHDKMRMIPVRLSARGKARLIKKRSVKPRRPKQYRPVKTGELIGMDAIELRMGDLRRYIIT
MIDEHSDYALALAVPSLNSDITSHFFSKATKLFPVAIRQVVTDNGKEFLGNFDKTLQEASIKHIWTYPYTPKMNATCERFNRTLREQFIEFNELLLFEDL
NLFNQRMAEYLVLYNSKRPHKSLELMTPVDYILRESKNCNMWWTH
Blast result :
Comments
ISKpn28 is 45% aa similar to ISCje1.
The transposase use the first STOP codon in the target site, we delete in silico the 14 last amino acid (TPPCKLHGKRPYWC).
The transposase use the first STOP codon in the target site, we delete in silico the 14 last amino acid (TPPCKLHGKRPYWC).
References
1] ISfinder annotation (2014)
2] McClelland,M., Sanderson,E.K., Spieth,J., Clifton,W.S., Latreille,P., Sabo,A., Pepin,K., Bhonagiri,V., Porwollik,S., Ali,J. and Wilson,R.K. (2006) Direct submission GenBank.
2] McClelland,M., Sanderson,E.K., Spieth,J., Clifton,W.S., Latreille,P., Sabo,A., Pepin,K., Bhonagiri,V., Porwollik,S., Ali,J. and Wilson,R.K. (2006) Direct submission GenBank.