ISHbo2
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001690 | ND | Halogeometricum borinquense | Halogeometricum borinquense DSM 11551 |
DNA section
IS Length : 1671 bp
Ends
Left end : ACGTAGCGAGCGGAAGCCCACCCCTTCAGGGGTGGGTCTGAGCGACACTATTCCGTCGCAAACAACGAGTTACGACTCGTCTGGATGTTCCACCGCTTCC II struct. : Yes
Right end : AACCAGAGTACCAGTGACGGGAAAATTGCCTCCGTGGATGCGGCGTAGCCAACATCCCCACCGGAGGAATCCCACGGCTTCAGCCGTGGGAGGATGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CGGCACTCAACA | TGAC | ACCGTGTCCTTC | tcaa |
DNA sequence
ACGTAGCGAGCGGAAGCCCACCCCTTCAGGGGTGGGTCTGAGCGACACTATTCCGTCGCAAACAACGAGTTACGACTCGTCTGGATGTTCCACCGCTTCC
AATCCCGCTTTGATTATCTCTCGGTAGGCTTCATCAAGAGCCATACCCTGTTCTTCGGCGTAGTCTCTCACACGCCCGTTCAGCGTGTGGGAAATGTCGA
TGTTCGGTCGCATCGCTTGTGTCCAAAAGACATTTAGACATAGAACTACTAATGTCTGCCGTCGTGAGGCGAACCAACACGTTCGCGGTACGACCGCTCT
CAGACGCCGACGAGCGGCTACTGCTGGACCTGTTGGACGCCTCTGCCAGCCTGTGGAACGAGTTGAACTACGAGCGCCGCCAGCAGTTCTTCGACGGCGA
CAGCGTGTGGGACACCGCCGACTACCGCAAACAGTACGTCGATGTGCTTGGTTCTGCCACCGCACAGCAGGTTATTCGGAAGAACAAATCAGCGTGGCAG
TCGTTTTTCGCCGCCCGCGAGAATGGCGAGGACACCGCCCCGCCGGGCTACTGGGGCAACGAGGACGACGGCAGAGAACTCCGCACGTACATCCGCAACG
ACCAATACACGCTGGAAACTGGCGACCGAAGTCGCCTCGAAATCCCGGTCGGCCAGCAGTTGAAAGATGAATACGGCCTCGGCTATCACGACCGCCTGCG
CCTCGAAGTTGCTGGCGACCCGAAGTGGGACGGCGAACAGGGCCGTCTGGAGATACAGTACGACGAGATAGACGACACGTTCAGGGCCTTTCAGCCTGTC
ACCGTGCCTGATTCTCGACAGGATTCACGGAAAATCGAAGATTTTCCTGAAGTCGAAAACGCTTCGCGTTTTCGGAATTCACCACTGGCTTCGGAAGAAG
CCGCTTTGGACGTGGGCGCGAACAATCTCGTCGCCTGTACCACCACGACCGGCCAGCAGTACCTCTACGAAGGACGGGACCTGTTTGCCCGCTTCCGCGA
GACGACCGATGAAATCGCCCGTCTCCAGTCCAAACTCCGCGAAGGACGGTACAGCAGTCGGCGGATACGGCGGCTGTATCGCAAACGGACGCGACGGCGC
GACCACGCGCAGGATGCACTGGTGCGCGACCTGCTGGAACGGCTTTACGCCGAGGGGGTCGCCACGGTGTACGTAGGCGACCTCACGGACGTTCTCTCGA
CGCATTGGTGCGCCGAGGTGAACGCCAAGACCCACCAGTTCTGGGCGTTTCAGGCGTTTATCGACCGCCTCTCGGACACCGCCGAGGAATACGGCATCAC
AGTCGAAGTGCGGTCTGAAGCCGACACAACCCGAACGTGCCCGGTGTGTGGCGAACAGGACGACACCGACCGGGACGGCGACGTGTTCCGGTGTCCGTGC
GGTCACGAAGCCCACGCCGACCTCTGTGCGTCTCGCACGTTCCTCGAACGACAGGCTGGCAAAATCAAAGTCGGGTCGATGGCTCGGCCCGTGCGTCTCA
AGTGGGACGACCACAACTGGTCGGAGATACCACACTCTCCCGAGAGGGAAAGTCCCAACGAGAAGCGCACAAACCAGAGTACCAGTGACGGGAAAATTGC
CTCCGTGGATGCGGCGTAGCCAACATCCCCACCGGAGGAATCCCACGGCTTCAGCCGTGGGAGGATGTCAA
AATCCCGCTTTGATTATCTCTCGGTAGGCTTCATCAAGAGCCATACCCTGTTCTTCGGCGTAGTCTCTCACACGCCCGTTCAGCGTGTGGGAAATGTCGA
TGTTCGGTCGCATCGCTTGTGTCCAAAAGACATTTAGACATAGAACTACTAATGTCTGCCGTCGTGAGGCGAACCAACACGTTCGCGGTACGACCGCTCT
CAGACGCCGACGAGCGGCTACTGCTGGACCTGTTGGACGCCTCTGCCAGCCTGTGGAACGAGTTGAACTACGAGCGCCGCCAGCAGTTCTTCGACGGCGA
CAGCGTGTGGGACACCGCCGACTACCGCAAACAGTACGTCGATGTGCTTGGTTCTGCCACCGCACAGCAGGTTATTCGGAAGAACAAATCAGCGTGGCAG
TCGTTTTTCGCCGCCCGCGAGAATGGCGAGGACACCGCCCCGCCGGGCTACTGGGGCAACGAGGACGACGGCAGAGAACTCCGCACGTACATCCGCAACG
ACCAATACACGCTGGAAACTGGCGACCGAAGTCGCCTCGAAATCCCGGTCGGCCAGCAGTTGAAAGATGAATACGGCCTCGGCTATCACGACCGCCTGCG
CCTCGAAGTTGCTGGCGACCCGAAGTGGGACGGCGAACAGGGCCGTCTGGAGATACAGTACGACGAGATAGACGACACGTTCAGGGCCTTTCAGCCTGTC
ACCGTGCCTGATTCTCGACAGGATTCACGGAAAATCGAAGATTTTCCTGAAGTCGAAAACGCTTCGCGTTTTCGGAATTCACCACTGGCTTCGGAAGAAG
CCGCTTTGGACGTGGGCGCGAACAATCTCGTCGCCTGTACCACCACGACCGGCCAGCAGTACCTCTACGAAGGACGGGACCTGTTTGCCCGCTTCCGCGA
GACGACCGATGAAATCGCCCGTCTCCAGTCCAAACTCCGCGAAGGACGGTACAGCAGTCGGCGGATACGGCGGCTGTATCGCAAACGGACGCGACGGCGC
GACCACGCGCAGGATGCACTGGTGCGCGACCTGCTGGAACGGCTTTACGCCGAGGGGGTCGCCACGGTGTACGTAGGCGACCTCACGGACGTTCTCTCGA
CGCATTGGTGCGCCGAGGTGAACGCCAAGACCCACCAGTTCTGGGCGTTTCAGGCGTTTATCGACCGCCTCTCGGACACCGCCGAGGAATACGGCATCAC
AGTCGAAGTGCGGTCTGAAGCCGACACAACCCGAACGTGCCCGGTGTGTGGCGAACAGGACGACACCGACCGGGACGGCGACGTGTTCCGGTGTCCGTGC
GGTCACGAAGCCCACGCCGACCTCTGTGCGTCTCGCACGTTCCTCGAACGACAGGCTGGCAAAATCAAAGTCGGGTCGATGGCTCGGCCCGTGCGTCTCA
AGTGGGACGACCACAACTGGTCGGAGATACCACACTCTCCCGAGAGGGAAAGTCCCAACGAGAAGCGCACAAACCAGAGTACCAGTGACGGGAAAATTGC
CTCCGTGGATGCGGCGTAGCCAACATCCCCACCGGAGGAATCCCACGGCTTCAGCCGTGGGAGGATGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1356 bp | 451 aa | 264 | 1619 | + | No |
AG : TnpB
ORF sequence :
MVRRTNTFAVRPLSDADERLLLDLLDASASLWNELNYERRQQFFDGDSVWDTADYRKQYVDVLGSATAQQVIRKNKSAWQSFFAARENGEDTAPPGYWGN
EDDGRELRTYIRNDQYTLETGDRSRLEIPVGQQLKDEYGLGYHDRLRLEVAGDPKWDGEQGRLEIQYDEIDDTFRAFQPVTVPDSRQDSRKIEDFPEVEN
ASRFRNSPLASEEAALDVGANNLVACTTTTGQQYLYEGRDLFARFRETTDEIARLQSKLREGRYSSRRIRRLYRKRTRRRDHAQDALVRDLLERLYAEGV
ATVYVGDLTDVLSTHWCAEVNAKTHQFWAFQAFIDRLSDTAEEYGITVEVRSEADTTRTCPVCGEQDDTDRDGDVFRCPCGHEAHADLCASRTFLERQAG
KIKVGSMARPVRLKWDDHNWSEIPHSPERESPNEKRTNQSTSDGKIASVDAA
EDDGRELRTYIRNDQYTLETGDRSRLEIPVGQQLKDEYGLGYHDRLRLEVAGDPKWDGEQGRLEIQYDEIDDTFRAFQPVTVPDSRQDSRKIEDFPEVEN
ASRFRNSPLASEEAALDVGANNLVACTTTTGQQYLYEGRDLFARFRETTDEIARLQSKLREGRYSSRRIRRLYRKRTRRRDHAQDALVRDLLERLYAEGV
ATVYVGDLTDVLSTHWCAEVNAKTHQFWAFQAFIDRLSDTAEEYGITVEVRSEADTTRTCPVCGEQDDTDRDGDVFRCPCGHEAHADLCASRTFLERQAG
KIKVGSMARPVRLKWDDHNWSEIPHSPERESPNEKRTNQSTSDGKIASVDAA
Blast result :
Comments
ISHbo2 is 86% aa (TnpB) similar to ISNpe11.
References
1] Pfeiffer F. (2013) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Anderson,I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Anderson,I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.