ISLgar2
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP009332.1 | ND | Lactococcus garvieae | Lactococcus garvieae ATCC49156 Lactococcus garvieae Lg2 |
DNA section
IS Length : 998 bp
Ends
IR Length : 20
IRL : ACCCGAATTGCTAGTTGATTTTATCACAAAATAATAAGCCGTGCTATCCT
IRR : ACCCGAATTGCTAGTTGATTATTTAGCCATGACTTGATATCCGATAGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTTTTTTAT | ACTTAAAT | CAATAAAATG | 8 |
| AAAAATATTC | TTTATATAT | ATTAGTTTGT | 9 |
| TCATTAATCC | TTACATAT | AGGAAATCTG | 8 |
| ATCCAAACCT | TTAAGTAT | ATATTCCCTA | 8 |
| GCTAAAATAA | AAGAGTT | ACAATTTCCG | 7 |
| GAAAAAATAC | TCTACATT | AAAAGATTTT | 8 |
| CATATATAAT | ATATATAATA | 0 |
DNA sequence
ACCCGAATTGCTAGTTGATTTTATCACAAAATAATAAGCCGTGCTATCCTAATCTTAAACCACTAAGAATTAGAAAGCGCACGACTTATATGATTTATAA
TAGCACACTTCCAAAAGTTTTTGTTTATTTGCTGACAGCTATCGAGAAGCTTTACCAAGAGACTGTTCCTCTTGAAGTTCAGAACCGAAAGAACATTCAT
CTTGCTACATCTGATTGTCTAGTGATTACTTGTTACCTGTGGGGAGTCATCCATTTTAGCGAGACGCTTAAAGCTAAACACCAACTCGCTCAAAGTTTAT
TTCCTGATTTTCTTGAATACTCCCGTTTTGTTCGTCGTTGTAATGCACTCTTGCCGAGTATTCAAGTCATTCGTCAAGCACTCGTCCTTAAAGAGATTGA
AGGGATTAGTGTGTCTATTATTGACAGCTTCCCCATTCCATTGTGTCAACCGATTCGTAATTTCAGAAGCAACGTTCTAGGGGAATATGCAAATATTGGA
TATAATGCGACCAAAGAACAGTACTTTTATGGCTGTAAATGCCATGCTTTAGTCAGTGAATCAGGATATGTTATCGACTACGTAATTACTCCTGCTTCAA
TCGCAGACAGTACAATGGCTGAGGAAGTTGTGGCTCATTTTGGAACAGCCACAGTACTTGGAGATATGGGATATTTAGGACATGCTCTCCATGATCGCCT
TAAACTTAAAGGAATTGAGCTGATCACACCAGTCAGGAAGAACATGAAGAAAAAAATTATCGCTTTTCCTGATTTTTCAAAACGTAGAAAAGTCATTGAA
CGAGTATTTTCTTTTCTGACCAACCTCGGAGCAGAGCGCTGTAAAAGTCGCTCTGCCTATGGCTTTCAAGTTAAATTAGAGATGTTACTTTTAGCATATT
CTTTACTGCTGAAATCAGCTAAATCCCTTAATTCTAAGACTTTAAGATATTCTATCGGATATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
TAGCACACTTCCAAAAGTTTTTGTTTATTTGCTGACAGCTATCGAGAAGCTTTACCAAGAGACTGTTCCTCTTGAAGTTCAGAACCGAAAGAACATTCAT
CTTGCTACATCTGATTGTCTAGTGATTACTTGTTACCTGTGGGGAGTCATCCATTTTAGCGAGACGCTTAAAGCTAAACACCAACTCGCTCAAAGTTTAT
TTCCTGATTTTCTTGAATACTCCCGTTTTGTTCGTCGTTGTAATGCACTCTTGCCGAGTATTCAAGTCATTCGTCAAGCACTCGTCCTTAAAGAGATTGA
AGGGATTAGTGTGTCTATTATTGACAGCTTCCCCATTCCATTGTGTCAACCGATTCGTAATTTCAGAAGCAACGTTCTAGGGGAATATGCAAATATTGGA
TATAATGCGACCAAAGAACAGTACTTTTATGGCTGTAAATGCCATGCTTTAGTCAGTGAATCAGGATATGTTATCGACTACGTAATTACTCCTGCTTCAA
TCGCAGACAGTACAATGGCTGAGGAAGTTGTGGCTCATTTTGGAACAGCCACAGTACTTGGAGATATGGGATATTTAGGACATGCTCTCCATGATCGCCT
TAAACTTAAAGGAATTGAGCTGATCACACCAGTCAGGAAGAACATGAAGAAAAAAATTATCGCTTTTCCTGATTTTTCAAAACGTAGAAAAGTCATTGAA
CGAGTATTTTCTTTTCTGACCAACCTCGGAGCAGAGCGCTGTAAAAGTCGCTCTGCCTATGGCTTTCAAGTTAAATTAGAGATGTTACTTTTAGCATATT
CTTTACTGCTGAAATCAGCTAAATCCCTTAATTCTAAGACTTTAAGATATTCTATCGGATATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 90 | 980 | + | No |
Chemistry : DDE
ORF sequence :
MIYNSTLPKVFVYLLTAIEKLYQETVPLEVQNRKNIHLATSDCLVITCYLWGVIHFSETLKAKHQLAQSLFPDFLEYSRFVRRCNALLPSIQVIRQALVL
KEIEGISVSIIDSFPIPLCQPIRNFRSNVLGEYANIGYNATKEQYFYGCKCHALVSESGYVIDYVITPASIADSTMAEEVVAHFGTATVLGDMGYLGHAL
HDRLKLKGIELITPVRKNMKKKIIAFPDFSKRRKVIERVFSFLTNLGAERCKSRSAYGFQVKLEMLLLAYSLLLKSAKSLNSKTLRYSIGYQVMAK
KEIEGISVSIIDSFPIPLCQPIRNFRSNVLGEYANIGYNATKEQYFYGCKCHALVSESGYVIDYVITPASIADSTMAEEVVAHFGTATVLGDMGYLGHAL
HDRLKLKGIELITPVRKNMKKKIIAFPDFSKRRKVIERVFSFLTNLGAERCKSRSAYGFQVKLEMLLLAYSLLLKSAKSLNSKTLRYSIGYQVMAK
Blast result :
Comments
ISLgar2 is 93% aa similar to ISLll1.
There are 7 and 10 identical copies of ISLgar2 along the genome of strains ATCC49156 and Lg2 respectively.
There are 7 and 10 identical copies of ISLgar2 along the genome of strains ATCC49156 and Lg2 respectively.
References
1] Eraclio, G., Ricci, G., Fortina, MG. (Paper in preparation)