ISLgar1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AGQY01000076.1 | ND | Lactococcus garvieae | Lactococcus garvieae LG9 |
DNA section
IS Length : 1431 bp
Ends
IR Length : 23/28
IRL : TGAACTGCACCCCAAAAGTTAGACAATAAATCTAACTTTTGGGGTGTTTT
IRR : TGAAGTGACCCCAAGAAGTTAGACAATATTTAAGCTAAGGATTGAGTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGAACTGCACCCCAAAAGTTAGACAATAAATCTAACTTTTGGGGTGTTTTTCTATGAAATTAAATGATGACGATAAAATTGAAATCTATCAGTTAAGATT
AGCAGGTTGGTCTTGGCCAAAGCTCAGTCGAAAATTTAATATCACCGAGTCTAACTTGCAATATATGGTACGTCTTATTGAGAAGCATGGAATAGAAAGT
GTCCGTAAAGGAAAAAATCAGGTTTATCCTCCTGAATTGAAGCAAGATATGATGGATGAAGTCCTTCTAAATGGTCGCTCTCAGCTAGAGGTTTCCCTAG
ATTATGGGTTGCCTAACAAGGGAATGCTTCCTAATTGGATAGCACAATACAAGAAAAACGGGTATACTATACTTGAGAAACCAAGAGGGAGACATGGAAA
GATGGGACGACAACCGAAGAAAAAACCAGAAGAGATGACCGAATTAGAGCGTCTTCAGTACGAAAATGAATACCTTAGAACGGAGAATGCCGTGCTAAAA
AAGCTGAGAGAATACCGCTTAAAGGACGAAGCAAAGCTCAAAGAGCAACAGAAATCATCCAAGGATTAATCAAAGAATTTAAGTTAACACTCTTGCTTGA
TACTTTGAAGTTATCTCGGTCAACTTACTATTATCAAATGAAACGACTGGCTCAAGAAGATAAAGACAGTGAGTTAAAAGAGACTATTCAAGACATCTAT
ACCGAGCACAAGGGGAGATATGGTTACCGAAGAATTCATCTTGAAATTAGAAATCGAGGCATCCAAGTCAACCATAAGAAAGTTCAACGTCTGATGACCG
AACTTGGTTTAAAAGCTAGGATTCGCGCTCAACGTCGTTACAATTCCTATAAGGGAGAGGTGGGTAAGAAAGCTAATAATCTCATTAAGCGCCAATTCAA
AGCGACACAACCTCTTGAGAAGTGTTATACTGATGTGACCGAATTTTCCATTCCTGCTAGTGATCAAAAGTTATATTTATCCCCGGTTCTTGATGGTTAT
AACAGTGAAATCATTGCTTATCATCTTTCTACGTCACCGAATTTACAACAACTAAAAACAATGCTTGAAGAGGCATTTCCAAAGCAAACTTACCAAGGAA
CCATCTTGCATAGTGATCAGGGGTGGCAATACCAACATGCCTATTATCATCAGTTTCTGGAGAAACATAGTATGAGACCATCAATGTCTCGCAAAGGCAA
TAGTTTAGACAATGGCATGATGGAATCTTTCTTTGGAACACTGAAAACTGAGATGTTCTATGGATTTGAGAAGGAATTTACTTCTCTTGAAGTATTAGAA
ACAGCCATTACAGAATATATTGACTATTACAACAACAAACGAATCAAACTAACATTAAAAGGACTGAGCCCTGTGCAATACAGAACTCAATCCTTAGCTT
AAATATTGTCTAACTTCTTGGGGTCACTTCA
AGCAGGTTGGTCTTGGCCAAAGCTCAGTCGAAAATTTAATATCACCGAGTCTAACTTGCAATATATGGTACGTCTTATTGAGAAGCATGGAATAGAAAGT
GTCCGTAAAGGAAAAAATCAGGTTTATCCTCCTGAATTGAAGCAAGATATGATGGATGAAGTCCTTCTAAATGGTCGCTCTCAGCTAGAGGTTTCCCTAG
ATTATGGGTTGCCTAACAAGGGAATGCTTCCTAATTGGATAGCACAATACAAGAAAAACGGGTATACTATACTTGAGAAACCAAGAGGGAGACATGGAAA
GATGGGACGACAACCGAAGAAAAAACCAGAAGAGATGACCGAATTAGAGCGTCTTCAGTACGAAAATGAATACCTTAGAACGGAGAATGCCGTGCTAAAA
AAGCTGAGAGAATACCGCTTAAAGGACGAAGCAAAGCTCAAAGAGCAACAGAAATCATCCAAGGATTAATCAAAGAATTTAAGTTAACACTCTTGCTTGA
TACTTTGAAGTTATCTCGGTCAACTTACTATTATCAAATGAAACGACTGGCTCAAGAAGATAAAGACAGTGAGTTAAAAGAGACTATTCAAGACATCTAT
ACCGAGCACAAGGGGAGATATGGTTACCGAAGAATTCATCTTGAAATTAGAAATCGAGGCATCCAAGTCAACCATAAGAAAGTTCAACGTCTGATGACCG
AACTTGGTTTAAAAGCTAGGATTCGCGCTCAACGTCGTTACAATTCCTATAAGGGAGAGGTGGGTAAGAAAGCTAATAATCTCATTAAGCGCCAATTCAA
AGCGACACAACCTCTTGAGAAGTGTTATACTGATGTGACCGAATTTTCCATTCCTGCTAGTGATCAAAAGTTATATTTATCCCCGGTTCTTGATGGTTAT
AACAGTGAAATCATTGCTTATCATCTTTCTACGTCACCGAATTTACAACAACTAAAAACAATGCTTGAAGAGGCATTTCCAAAGCAAACTTACCAAGGAA
CCATCTTGCATAGTGATCAGGGGTGGCAATACCAACATGCCTATTATCATCAGTTTCTGGAGAAACATAGTATGAGACCATCAATGTCTCGCAAAGGCAA
TAGTTTAGACAATGGCATGATGGAATCTTTCTTTGGAACACTGAAAACTGAGATGTTCTATGGATTTGAGAAGGAATTTACTTCTCTTGAAGTATTAGAA
ACAGCCATTACAGAATATATTGACTATTACAACAACAAACGAATCAAACTAACATTAAAAGGACTGAGCCCTGTGCAATACAGAACTCAATCCTTAGCTT
AAATATTGTCTAACTTCTTGGGGTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 516 bp | 171 aa | 54 | 569 | + | No |
Description : First part of the transposase
ORF sequence :
MKLNDDDKIEIYQLRLAGWSWPKLSRKFNITESNLQYMVRLIEKHGIESVRKGKNQVYPPELKQDMMDEVLLNGRSQLEVSLDYGLPNKGMLPNWIAQYK
KNGYTILEKPRGRHGKMGRQPKKKPEEMTELERLQYENEYLRTENAVLKKLREYRLKDEAKLKEQQKSSKD
KNGYTILEKPRGRHGKMGRQPKKKPEEMTELERLQYENEYLRTENAVLKKLREYRLKDEAKLKEQQKSSKD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 479 | 1402 | + | No |
Description : Second part of the transposase
ORF sequence :
NGECRAKKAERIPLKGRSKAQRATEIIQGLIKEFKLTLLLDTLKLSRSTYYYQMKRLAQEDKDSELKETIQDIYTEHKGRYGYRRIHLEIRNRGIQVNHK
KVQRLMTELGLKARIRAQRRYNSYKGEVGKKANNLIKRQFKATQPLEKCYTDVTEFSIPASDQKLYLSPVLDGYNSEIIAYHLSTSPNLQQLKTMLEEAF
PKQTYQGTILHSDQGWQYQHAYYHQFLEKHSMRPSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTSLEVLETAITEYIDYYNNKRIKLTLKGLSPVQ
YRTQSLA
KVQRLMTELGLKARIRAQRRYNSYKGEVGKKANNLIKRQFKATQPLEKCYTDVTEFSIPASDQKLYLSPVLDGYNSEIIAYHLSTSPNLQQLKTMLEEAF
PKQTYQGTILHSDQGWQYQHAYYHQFLEKHSMRPSMSRKGNSLDNGMMESFFGTLKTEMFYGFEKEFTSLEVLETAITEYIDYYNNKRIKLTLKGLSPVQ
YRTQSLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1349 bp | 449 aa | 54 | 1402 | + | Yes |
Chemistry : DDE
ORF sequence :
MKLNDDDKIEIYQLRLAGWSWPKLSRKFNITESNLQYMVRLIEKHGIESVRKGKNQVYPPELKQDMMDEVLLNGRSQLEVSLDYGLPNKGMLPNWIAQYK
KNGYTILEKPRGRHGKMGRQPKKKPEEMTELERLQYENEYLRTENAVLKKAERIPLKGRSKAQRATEIIQGLIKEFKLTLLLDTLKLSRSTYYYQMKRLA
QEDKDSELKETIQDIYTEHKGRYGYRRIHLEIRNRGIQVNHKKVQRLMTELGLKARIRAQRRYNSYKGEVGKKANNLIKRQFKATQPLEKCYTDVTEFSI
PASDQKLYLSPVLDGYNSEIIAYHLSTSPNLQQLKTMLEEAFPKQTYQGTILHSDQGWQYQHAYYHQFLEKHSMRPSMSRKGNSLDNGMMESFFGTLKTE
MFYGFEKEFTSLEVLETAITEYIDYYNNKRIKLTLKGLSPVQYRTQSLA
KNGYTILEKPRGRHGKMGRQPKKKPEEMTELERLQYENEYLRTENAVLKKAERIPLKGRSKAQRATEIIQGLIKEFKLTLLLDTLKLSRSTYYYQMKRLA
QEDKDSELKETIQDIYTEHKGRYGYRRIHLEIRNRGIQVNHKKVQRLMTELGLKARIRAQRRYNSYKGEVGKKANNLIKRQFKATQPLEKCYTDVTEFSI
PASDQKLYLSPVLDGYNSEIIAYHLSTSPNLQQLKTMLEEAFPKQTYQGTILHSDQGWQYQHAYYHQFLEKHSMRPSMSRKGNSLDNGMMESFFGTLKTE
MFYGFEKEFTSLEVLETAITEYIDYYNNKRIKLTLKGLSPVQYRTQSLA
Blast result :
Comments
ISLgar1 is 82% aa similar to ISSth6. The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Eraclio G., Ricci G., Fortina MG. (2014) Paper in preparation