ISLla3

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003132 ND Lactococcus lactis
Enterococcus faecalis 62
Lactococcus lactis subsp. cremoris SK11
Lactococcus lactis subsp. cremoris A76
Lactococcus lactis subsp. cremoris A76 pQA554
Lactococcus lactis subsp. cremoris UC5099
Lactococcus lactis subsp. lactis IL1403
Lactococcus lactis subsp. lactis pK214
Lactococcus garvieae TB25
DNA section
IS Length : 1484 bp

Ends


IR Length : 23/30

IRL : TGTAGTGACCCCCAAAATATAGACTTTTCAAATCTATATTTCGGAGGTCT
IRR : TGATGTGCACCCCAAAAGTTAGACTTTTTATCCAAGTAGAAATACTTGGA

Insertion site


Left flankDirect repeatRight flankDR Length
ATAACCGTGAATTTCGTTCACTT3
AAAAACCGCTTTTGGCGGTTTTT3
AAGCCTCTAAATTAGAGGCTTTT3
AAAAACCGTTACTCACGGCTTTC3
ACCTGAGTAAATTTGCTGGC0
AAAAACCGCCAAAAGCGGTTTTT3
TGGAACCTTTTTGAGCGGCTACT3
GCTTTTTTGAGTTGACGGTTCTT3

DNA sequence

TGTAGTGACCCCCAAAATATAGACTTTTCAAATCTATATTTCGGAGGTCTTTTCTTATGGTCAAATATTCCATAGAATTAAAACAACGCGTTATTCAAGA
TTATTTATCTGGCAAGGGAGGCTCTACCTATCTTGCTAAACTGCACAACGTAGGTTCATCTAGTCAGGTTAGACGCTGGATTCGCAATTATCGAGCAGAG
GGACTTCCTACAGCTCACTCCAAAGTCAATAAAAATTATTCTATGGAGTTGAAAGAAAATGCGGTACAATGTTATTTAACAACTGATTTAACGTATGAAG
CTGTGGCAAGAAAATTCGAAATTACTAATTTTACCTTACTTGCAAGCTGGGTTAATCATTTCAAAATTTATGGAGAAGTCCCAATAAGCAAAAAGAGAGG
ACGGCGTAAAAAACTAGAAAGCATTGCATCGTCTATGACTCAGAACCCAAATGATTCTCAACGAATTAAAGAACTTGAACAAGAATTACGTTATGCGCAA
ATTGAGGTAGCTTATTTAAAAGGACTTCGGAGATTGGAGAAAAATGCTCTAATGAACAAAAATCAAGACTCATCTACAGTCTCCGTAAAACCTTCAAACT
CAAAGAAATCTTGAAAGTTACAGGATTCCCTAAAGCTACTTATTATTATTGGGTTAACTGTTTTGAACGGGTAAACAAAGATGAGCTTATCGAAAAAGAA
ATGCTTAAAATACGTCAAGAACATGCCAATGCAGGTTATCGTCCAATGAGTGAATTACTTAAGCAACGTGGCTATCACGTCAACCACAAGAAGGTACAGC
GCTTAATGAAGAAGCTAGGGCTTCGTGTGACGTCTTATTGGCACAAATCACGTAAATATAATTCATATAAAGGAAAAGTAGGGACGGTAGCTAAAAATAA
ATTGCACAGACGATTCAGAACTTCCATTCCTCATCAAAAAATCACAACAGATACGACAGAATTCAAATATTATGAAGATGGAATTCAGAAAAAGTGTTAT
CTCAATCCTTACATTGATTTATTTAATAGCGAGGTGATTAGTTATCATATCTCTAAACAACCCTCTTATCAATCAATTGATATTGCTCTAAATCAAGCCT
TAGCTGTGACATCTGATTGTCCTTATCGACGTACTTTCCACTCAGATCAAGGATGGGGATACCAAATGAGAGACTATGTTTCTAAATTAAAATCTCATAG
AATTTTTCAGTCTATGAGCCGTAAAGGAAACTGTCATGATAATTCAGTTATGGAGAATTTCTTTGGGCTACTTAAACAAGAAATTTACTACGGACATATC
TTCTCGTCTTTCGAAGAACTTGAACAGGTTATTGTAATCTGGATTAGGTACTATAATACAAAACGAATCAAACAAAAATTGAACTGGATGAGTCCAATTC
AATTTCGCTTGAATTACCAAAACAATTAAAAAAATCCAAGTATTTCTACTTGGATAAAAAGTCTAACTTTTGGGGTGCACATCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
558 bp185 aa57614+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVKYSIELKQRVIQDYLSGKGGSTYLAKLHNVGSSSQVRRWIRNYRAEGLPTAHSKVNKNYSMELKENAVQCYLTTDLTYEAVARKFEITNFTLLASWVN
HFKIYGEVPISKKRGRRKKLESIASSMTQNPNDSQRIKELEQELRYAQIEVAYLKGLRRLEKNALMNKNQDSSTVSVKPSNSKKS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
924 bp307 aa5061429+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GSLFKRTSEIGEKCSNEQKSRLIYSLRKTFKLKEILKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMSELLKQRGYHVNHKKVQRL
MKKLGLRVTSYWHKSRKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKQPSYQSIDIALNQALA
VTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQVIVIWIRYYNTKRIKQKLNWMSPIQF
RLNYQNN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1373 bp457 aa571429+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVKYSIELKQRVIQDYLSGKGGSTYLAKLHNVGSSSQVRRWIRNYRAEGLPTAHSKVNKNYSMELKENAVQCYLTTDLTYEAVARKFEITNFTLLASWVN
HFKIYGEVPISKKRGRRKKLESIASSMTQNPNDSQRIKELEQELRYAQIEVAYLKRTSEIGEKCSNEQKSRLIYSLRKTFKLKEILKVTGFPKATYYYWV
NCFERVNKDELIEKEMLKIRQEHANAGYRPMSELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKSRKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDT
TEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKQPSYQSIDIALNQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVME
NFFGLLKQEIYYGHIFSSFEELEQVIVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

 

Blast result :
Comments
ISLla3 is 57% aa similar to ISEnfa3.
The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Eraclio,G., Ricci,G., Fortina, M.G. (2014) Paper in preparation
2] Eraclio,G. (2014) Direct submission to ISfinder