ISSag12
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ507131 | ND | Streptococcus agalactiae | Streptococcus agalactiae |
DNA section
IS Length : 1369 bp
Ends
IR Length : 28/40
IRL : TGAATAGGCTACACTTAACTAGACAGAAAAGATAAGGTGTGTAAAATATA
IRR : TGGTTTGGCAACACTTTTTTAGACAAATTTTATAAGGTGTTCTTTTCATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAATATATT | GAC | AATACTGATA | 3 |
DNA sequence
TGAATAGGCTACACTTAACTAGACAGAAAAGATAAGGTGTGTAAAATATAAGAAAACACACTGGAGGAAATATCACATGACTAGACGTGTACGTCAAATT
TATACCCCTGAATTTAAACAACAAATGGTTGATCTTTATCAAGCTGGAAAACCCAGAGCACAGATTATCAAAGAATATGATTTAACGCCTTCTGCGTTTG
ACCGTTGGATTCGACAATCCAAACAGACCGGTAGTTTTGAGACGAAAGATAATTTAACAATGGATCAAAAAGAATTAATTGAATTACGTAAACGAAATAA
ACAGCTTGAAATGGAAAATGATATTTTAAAGCAAGCGGCGCTGATATTCGGACGAAGAGACAAGTAATTGATCAAAATAAAGATCGTTACTCGATATCAG
CGATGTGCAAAGTACTTGGCATCTCTCGACAAACGTATTACTACCAACCAAAAGAAAAACATTCAGAGGCTGAGTTAGAAGAACAAGTCGAAGAGTTATT
TATGGAAAACCGAAAAATTTGTGGGACAAGAAAGTTGAAAAATCAGCTTGAAAAACGCGCCATCACCATCAGTCGTCGAAAAATCGGAAGTATTATGAAA
AGACGAAATTTACAATCTGCCTATACCGTTGCTCAATTTAAAGCACCAAAATCGTCTGTAAACGAAGCTCCCACTCCTAATTTATTGAATCGGGAATTTT
CTGATCGCGAACCATTAGAAGCGATTGTCACTGATCTAACTTATGTTCGAGTAGGAAAGGCTTGGCATTATATTTGTGCCATTTTAGACTTATTTAATCG
AGAAATTATCGGGTATTCTTGCGGACCTAGAAAAGATGCACAATTGGTCAAAAAAGCCTTTCAAACGATTAATTATCCATTGAATGACGTGGCGATTTTC
CACACTGATCGAGGCAAAGAATTCGATAACCAGTTGATTGACCAACTCCTAGAGTCTTTTGAGATTGAACGTTCTTTGAGTCGTAAGGGCAATCCTTATG
ACAATGCAGTAGCGGAATCTACCTATAAGTCAATGAAAATTGAATTCATCCGTCAACACACTTTTGAAACGTTAGACGAACTACGTATTCAGCTTTCTGA
CTACATTCATTGGTGGAACTATCTGCGAATCCATGGTTCATTGGACAATCAAACACCGATTGCCTATCGGCAACAGAGATTGGCGAAGCGAACCCTTGAT
AATGAGCATGGGTGTGATAGCCAAAGGCAGACGGCATAATCATTTGTATTTGCCGACTTCGTCTGAACTCATACGCATGGAGGCTCGTTGTCAAGGGCAA
TCGGAGGCTTCGGATAGATAATGAAAAGAACACCTTATAAAATTTGTCTAAAAAAGTGTTGCCAAACCA
TATACCCCTGAATTTAAACAACAAATGGTTGATCTTTATCAAGCTGGAAAACCCAGAGCACAGATTATCAAAGAATATGATTTAACGCCTTCTGCGTTTG
ACCGTTGGATTCGACAATCCAAACAGACCGGTAGTTTTGAGACGAAAGATAATTTAACAATGGATCAAAAAGAATTAATTGAATTACGTAAACGAAATAA
ACAGCTTGAAATGGAAAATGATATTTTAAAGCAAGCGGCGCTGATATTCGGACGAAGAGACAAGTAATTGATCAAAATAAAGATCGTTACTCGATATCAG
CGATGTGCAAAGTACTTGGCATCTCTCGACAAACGTATTACTACCAACCAAAAGAAAAACATTCAGAGGCTGAGTTAGAAGAACAAGTCGAAGAGTTATT
TATGGAAAACCGAAAAATTTGTGGGACAAGAAAGTTGAAAAATCAGCTTGAAAAACGCGCCATCACCATCAGTCGTCGAAAAATCGGAAGTATTATGAAA
AGACGAAATTTACAATCTGCCTATACCGTTGCTCAATTTAAAGCACCAAAATCGTCTGTAAACGAAGCTCCCACTCCTAATTTATTGAATCGGGAATTTT
CTGATCGCGAACCATTAGAAGCGATTGTCACTGATCTAACTTATGTTCGAGTAGGAAAGGCTTGGCATTATATTTGTGCCATTTTAGACTTATTTAATCG
AGAAATTATCGGGTATTCTTGCGGACCTAGAAAAGATGCACAATTGGTCAAAAAAGCCTTTCAAACGATTAATTATCCATTGAATGACGTGGCGATTTTC
CACACTGATCGAGGCAAAGAATTCGATAACCAGTTGATTGACCAACTCCTAGAGTCTTTTGAGATTGAACGTTCTTTGAGTCGTAAGGGCAATCCTTATG
ACAATGCAGTAGCGGAATCTACCTATAAGTCAATGAAAATTGAATTCATCCGTCAACACACTTTTGAAACGTTAGACGAACTACGTATTCAGCTTTCTGA
CTACATTCATTGGTGGAACTATCTGCGAATCCATGGTTCATTGGACAATCAAACACCGATTGCCTATCGGCAACAGAGATTGGCGAAGCGAACCCTTGAT
AATGAGCATGGGTGTGATAGCCAAAGGCAGACGGCATAATCATTTGTATTTGCCGACTTCGTCTGAACTCATACGCATGGAGGCTCGTTGTCAAGGGCAA
TCGGAGGCTTCGGATAGATAATGAAAAGAACACCTTATAAAATTTGTCTAAAAAAGTGTTGCCAAACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 77 | 367 | + | No |
Description : First part of the transposase
ORF sequence :
MTRRVRQIYTPEFKQQMVDLYQAGKPRAQIIKEYDLTPSAFDRWIRQSKQTGSFETKDNLTMDQKELIELRKRNKQLEMENDILKQAALIFGRRDK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 322 | 1239 | + | No |
Description : Second part of the transposase
ORF sequence :
YFKASGADIRTKRQVIDQNKDRYSISAMCKVLGISRQTYYYQPKEKHSEAELEEQVEELFMENRKICGTRKLKNQLEKRAITISRRKIGSIMKRRNLQSA
YTVAQFKAPKSSVNEAPTPNLLNREFSDREPLEAIVTDLTYVRVGKAWHYICAILDLFNREIIGYSCGPRKDAQLVKKAFQTINYPLNDVAIFHTDRGKE
FDNQLIDQLLESFEIERSLSRKGNPYDNAVAESTYKSMKIEFIRQHTFETLDELRIQLSDYIHWWNYLRIHGSLDNQTPIAYRQQRLAKRTLDNEHGCDS
QRQTA
YTVAQFKAPKSSVNEAPTPNLLNREFSDREPLEAIVTDLTYVRVGKAWHYICAILDLFNREIIGYSCGPRKDAQLVKKAFQTINYPLNDVAIFHTDRGKE
FDNQLIDQLLESFEIERSLSRKGNPYDNAVAESTYKSMKIEFIRQHTFETLDELRIQLSDYIHWWNYLRIHGSLDNQTPIAYRQQRLAKRTLDNEHGCDS
QRQTA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 77 | 1239 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRRVRQIYTPEFKQQMVDLYQAGKPRAQIIKEYDLTPSAFDRWIRQSKQTGSFETKDNLTMDQKELIELRKRNKQLEMENDILKASGADIRTKRQVIDQ
NKDRYSISAMCKVLGISRQTYYYQPKEKHSEAELEEQVEELFMENRKICGTRKLKNQLEKRAITISRRKIGSIMKRRNLQSAYTVAQFKAPKSSVNEAPT
PNLLNREFSDREPLEAIVTDLTYVRVGKAWHYICAILDLFNREIIGYSCGPRKDAQLVKKAFQTINYPLNDVAIFHTDRGKEFDNQLIDQLLESFEIERS
LSRKGNPYDNAVAESTYKSMKIEFIRQHTFETLDELRIQLSDYIHWWNYLRIHGSLDNQTPIAYRQQRLAKRTLDNEHGCDSQRQTA
NKDRYSISAMCKVLGISRQTYYYQPKEKHSEAELEEQVEELFMENRKICGTRKLKNQLEKRAITISRRKIGSIMKRRNLQSAYTVAQFKAPKSSVNEAPT
PNLLNREFSDREPLEAIVTDLTYVRVGKAWHYICAILDLFNREIIGYSCGPRKDAQLVKKAFQTINYPLNDVAIFHTDRGKEFDNQLIDQLLESFEIERS
LSRKGNPYDNAVAESTYKSMKIEFIRQHTFETLDELRIQLSDYIHWWNYLRIHGSLDNQTPIAYRQQRLAKRTLDNEHGCDSQRQTA
Blast result :
Comments
ISSag12 (orfAB) is 81% aa similar to IS1485.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The streptothricin resistance sat4 gene is truncated by ISSag12 element.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The streptothricin resistance sat4 gene is truncated by ISSag12 element.
References
1] Beatriz Rojo Bezares (2014) Direct submission to ISfinder