ISIde2

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Ideonella dechloratans
Ideonella dechloratans
DNA section
IS Length : 2529 bp

Ends


IR Length : 45/50

IRL : TGCGTATTCCAGACCAAACTGGACACCGATTCCACGGCAAACTGGACAGT
IRR : TGCGTATTCCACGCAAACTGGACAGCCAGTCCACGGCAAACTGGACAGTC

Insertion site


Left flankDirect repeatRight flankDR Length
GCTGAATTAGGCGTGTCGAG0

DNA sequence

TGCGTATTCCAGACCAAACTGGACACCGATTCCACGGCAAACTGGACAGTGATTCCAGAGCAATCTGGACAGTAAAAATAGTAACGTAGCGCGCGGGATA
ACCGTGGCACTCTCGGAGATTTTTCTTCGGAGACCACATGGCGAACAACAGGTTATCCATGCGCAAAATTACAGAAGCACTACGGCTGCATTTCGAGCAC
GACCGCACCAACCGTGAAATCGCCCTGGTCATCGGCACCTCGCCGACCACCGTCGGGGAATATCTGCGTCGAGCCCGTGTGGCTGGCATTGGTTATCCCT
TGCCAGACGGGCTGGATGACGCCGCCATTGAGTCCCGCCTCTTCCCGCCGGCAGTATCGGCGGAGGTCATCCGCCTGGAGCCCGACTGGGCATGGGTGCA
CCGAGAGATGCGCAAGAAGAGCGTCACGCTCGACTTGCTGTGGCAGGAATACAAGGCGGATCACCCGGATGGTTACCTATATAGTTGGTTCTGCGAACAT
TACCGACAGTGGGCCGGCAAGCTGTCGGTCAGCATGCGGCAGACGCACACCCCCGGCGAGAAACTGTTTGTCGACTATGTCGGCCAGACGCTGTCGATCA
TTGATGGCGCCACTGGTGAAATTCGCCAGGCACAACTGTTCGTCGCCGTCCTCGGCGCATCCAATTACACCTATGCCGAAGCGACCTGGACGCAGAAACT
GCCGGACTGGATTGGCTCCCATGTCCGCACCTTCGAGTTCCTTGGCGGCGTCACTGCGATCCTTGTCCCAGACAACCTCAAGTCAGGCGTCAAACATGCC
TCCTACTACGATCCGGAACTCAATCCGACCTATCAGGACTTCGCCCGGCATTACGGCGTAGCAGTGCTGCCGGCACGTCCATACAAGCCCAAGGACAAAG
CCAAGGTCGAAGGCGGCGTGCTGATTGTGGAACGCTGGATACTGGCGCGGCTGCGCAATCAACGCTTCTTCAGTTTGGCGGAAGCCAACCGCGCCATCGC
CCAATTGTTGGAGTCGCTGAATCAGCGGCCGTTCAAGAAACTGCCTGGCTGCCGCCACAGCGCCTTCCTTGAGATGGATCTCCCTGCGCTCAATCCCCTC
CCGCAATGCCGCTATGAATACGCCGAATGGAAGAAGGTGCGCGTTGGCGTTGATTACCACGTTGAAGGTGATGAGCATTACTACTCGGTGCCTTACCGCC
TTTCCCGCCTGCAATTGGAGCTGCGCATGACCGTCACCACGGTGGAGGTGTTTCATCGAGGCCAGCGCATTGCCGCACACGTGCGCAGCCCGATCAAAAA
TCACCACACCACGCTGGATGCGCACATGCCCCCGGAACACCAAGCCGTTGCCGGCTGGAATGGCCCGCGGTTGCTCGACTGGGCACAGAAGATTGGTAGC
TCCACTGAATCCGCGGTGCGAGTCATGCTGGGCGCGCGCAAGCATCCACAACAAAGCTATCGCGCCTGCCTCGGCGTACTGCGTCTGGCCAAGGGCTTTG
GCAATGATCGTTTAAATGCTGCGTGTGCTCGTGCTCTGAAACTGAACGCGGCGGGCTACCGCAGCATTCATTCAATACTCAAGAACGGCCTGGACCGGCA
ACAGCCGGCGATAGCCATTCAGGCATACCTGCCACTGGATCACGCCAATGTGCGTGGCCCGAACTATTACCATTAACACCACAGGAGAACCACCCATGCT
CAATCATCCAACACACGACCAACTCCAGCAATTACGCCTGTTCGGAATGGCACGCGCCCTCGCCGAACAGCAGGCCATTCCCGACATTACGCATCTGGCA
TTCGAGGAACGCCTCGGCCTCTTGGTTGAGCGCGAAGTCACTGAGCGTGATTCCCGACTAACTTCCAGCCGGCTACGGCGTGCAAAGCTCAAACAGGCAG
CCGTTGCCGAGGACGTGGACTACCGCGCAGCACGAAGCCTGGACCGCACCTTATTCAACCGGCTATTGACCGGCTCGTGGGTAGCAGAACACCAGAATGT
GCTGATCACAGGCCCCACGGGCGTCGGCAAGACTTGGCTGGCCTGCGCTCTGGCCAACCAAGCGTGCCGTCAGGGCAAATCGGTGCTTTACCTGCGGGTG
CCGCGATTGTTCGAGGATGTGGCGATTGCTCATGGGGACGGGCGTTACGGCAAGCTGATGATGCAACTCGCCAAGACCGATGTGCTGCTATTGGACGACT
GGGGATTGGCGTTACTGACCGACTCCGCGCGACGTGACTTGCTGGAAATCTTTGACGACCGGCACGGCCACCGTTCAACCATCGTAACCAGTCAATTGCC
GGTGGCGAACTGGCATGAATCCATCGGCGACCCCACCTTGGCTGACGCCATTCTGGATCGCCTGGTTCACAACGCTCATCAATTAATTCTGACCGGAGAA
TCCATGAGAAAACACAAAAATAGTTTGACCCAAAAACAAGTTTCAGAGTAAAACTATAACCCCCGCGTCGCTACGCTCCGACTGTCCAGTTTGCCGTGGA
CTGGCTGTCCAGTTTGCGTGGAATACGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1539 bp512 aa1381676+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MANNRLSMRKITEALRLHFEHDRTNREIALVIGTSPTTVGEYLRRARVAGIGYPLPDGLDDAAIESRLFPPAVSAEVIRLEPDWAWVHREMRKKSVTLDL
LWQEYKADHPDGYLYSWFCEHYRQWAGKLSVSMRQTHTPGEKLFVDYVGQTLSIIDGATGEIRQAQLFVAVLGASNYTYAEATWTQKLPDWIGSHVRTFE
FLGGVTAILVPDNLKSGVKHASYYDPELNPTYQDFARHYGVAVLPARPYKPKDKAKVEGGVLIVERWILARLRNQRFFSLAEANRAIAQLLESLNQRPFK
KLPGCRHSAFLEMDLPALNPLPQCRYEYAEWKKVRVGVDYHVEGDEHYYSVPYRLSRLQLELRMTVTTVEVFHRGQRIAAHVRSPIKNHHTTLDAHMPPE
HQAVAGWNGPRLLDWAQKIGSSTESAVRVMLGARKHPQQSYRACLGVLRLAKGFGNDRLNAACARALKLNAAGYRSIHSILKNGLDRQQPAIAIQAYLPL
DHANVRGPNYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa16962451+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLNHPTHDQLQQLRLFGMARALAEQQAIPDITHLAFEERLGLLVEREVTERDSRLTSSRLRRAKLKQAAVAEDVDYRAARSLDRTLFNRLLTGSWVAEHQ
NVLITGPTGVGKTWLACALANQACRQGKSVLYLRVPRLFEDVAIAHGDGRYGKLMMQLAKTDVLLLDDWGLALLTDSARRDLLEIFDDRHGHRSTIVTSQ
LPVANWHESIGDPTLADAILDRLVHNAHQLILTGESMRKHKNSLTQKQVSE

 

Blast result :
Comments
ISIde2 is 71% (istA, the transposase) and 76% (istB, helper of transposition) aa similar to ISSpu5.
References
1] Iain C. Clark (2014) Direct submission to ISfinder
2] Iain C. Clark, Ryan A. Melnyk, Anna Engelbrektson, John D. Coates (2013) mBio. Jul-Aug; 4(4): e00379-13