ISRme20
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007973 | ND | Ralstonia metallidurans | Ralstonia metallidurans CH34 Cupriavidus metallidurans CH34 |
DNA section
IS Length : 1977 bp
Ends
IR Length : 13/15
IRL : TGACCCAGCGGCAGCACATTGGATTTGACCCACCCGGGTAGGATGGGCCC
IRR : TGACCCTGGGTCAGCGTCAGTTGCTGGCCATCGGTTTCGCCTGACCCAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGTTGAAAA | CGCAACATGC | 0 |
DNA sequence
TGACCCAGCGGCAGCACATTGGATTTGACCCACCCGGGTAGGATGGGCCCTTTTTGGAGGGCCAGTGCTTACAAGGGAGGTAATCGTGGAAATACGTGTG
TTGGCCCGCCAAGGATTAGGAATCCGGGAGATCTCGCGGGAACTGGGGCTGTCGAGGAACACCGTGCGCAAATACTTACGCAGTTGTGCTGAGCAGGATC
GTGCGCCGCGATCGGGGCGCACGCAGAAGCTCGATCCGTTCAAAACCTATCTGCATGACCGGGTTCGCGCAGCGCATCCGGCCTGGCTGCCGGCGACCGC
GCTGCTGATCGAGATTCGCGCGATGGGTTATGACGGAGGCCTGACGCGGCTGCGGGTCTATCTGCGGTCGCTGAAGTCGATCAAGGCGCCGGAGCCACTG
GTACGTTTCGAAACCGATCCGGGGCAGCAGATGCAGGTCGACTGGATCGTGTTTCGACGTGGCAAGCTGCCGCTGTCGGCATTCGTTGCGACGCTCGGTT
ACAGTCGGGCGAGTTACGTCGAGTTCGTGACCGACGAACGATTGCCGACGCTGCTGGGATGCCATGAGCGCGCGTTCGACTTCTTCGGTGGGGTTCCTCG
CGAGGTCCTGTACGACAACGTCAAGACGGTGGTGATTGGCCGAGACGCCTATGGTCCCGGACTGCATCGCTATCAACCGGCCTTCCTCGACTTTGCGAAG
CACCACGGCTTCGTGCCGCGCCTGTGCAAGCCCTACCGAGCGAAGACCAAGGGGAAGGTTGAGCGCTTCAACGGATACCTGCGCCGCAGCTTCTACAATC
CGCTGGCAAGCCGGCTCGCTCAGGATCGCCTCTCGCTTGACGCGACCACGGCGAACGCCGAGGTCGTGCGCTGGCTGAGAGATGTCGCCAATGTGCGCAT
CCACGGCACGACTGGACAGTCGCCGCTCGAGCGTCTCCTGCTTGAGCAACCGAAGCTGCAGCAGTTGCCGGAGCCGTGGCGCGCGAACCTGCCGTCAGCT
TCCGTGCCGACGATGCCACCGCCGTCGCGGTTCGATGATACGCCGCTGCAGCATCAGCTGTCAGTGTATCAAGCCCTGCTGACGGAGGCACGTTGATGGA
CATCCAGCACGAACGTATCCTCGAAGCCTGTCGACAACTGCGGATCGACACGATGGGCGAGCAGTACAGCATGTTGGCAGCTCGCGCGGCAGCGAACCAA
CTCACGTTTGCCGACTTCTTCGAGGCGCTGTTGAAATCTGCGATGGACGCGAAGCACACGCGGACCAAATCCATGTTGCTGCGCACGGCCGGCTTCCCTG
TGCTGAAGACGCTGGAGGAGTTCGACTTTACGTTTGCTCACGGCGTTGCCAAGAAATCGGTGCTGGAGCTGTCGTCGATGGCCTTCGTCGAGCGCGCCGA
GAACATCGTGTTGCTCGGGCCGAGCGGCCTTGGCAAAACGCATCTGGCGATCGCACTCGGTTACATCGCAACGCAGATGGGGATCAAGACGCGCTTCATC
TCTGCGGCGGATCTCGTCATTGCCTTGGCCGCCGCTTCCCGGCAGGATCGATTGGCCGACTTCCTGAAGCGCAACATCATGAACCCACGGCTGTTGATCA
TTGATGAGGTCGGCTATCTGCCGCTCGGCCGGGACGATGCCAATCTGTTCTTCCAGGTGATCGCCAAGCGGTATGAAAAGGGTTCCGTGATCGTAACGAG
CAACTTGCCGTTCGGGCAATGGGACCAGACGTTCGCCGGAGATCAGACGCTCACGGCCGCGTTGCTCGACCGCCTCCTGCATCACAGCCATGTTCTACAG
ATCAAAGGCGAAAGCTACCGACTGAAGGACAAGCGAAAAGCCGGCGTGATCCGATCCCGTGCATCCGAAAGTGCTGAACAGCAACTGGAGATGCACGCGT
AAGTGATGATCAGCAACATCGTTTGACCCTGGGTCAGGCGAAACCGATGGCCAGCAACTGACGCTGACCCAGGGTCA
TTGGCCCGCCAAGGATTAGGAATCCGGGAGATCTCGCGGGAACTGGGGCTGTCGAGGAACACCGTGCGCAAATACTTACGCAGTTGTGCTGAGCAGGATC
GTGCGCCGCGATCGGGGCGCACGCAGAAGCTCGATCCGTTCAAAACCTATCTGCATGACCGGGTTCGCGCAGCGCATCCGGCCTGGCTGCCGGCGACCGC
GCTGCTGATCGAGATTCGCGCGATGGGTTATGACGGAGGCCTGACGCGGCTGCGGGTCTATCTGCGGTCGCTGAAGTCGATCAAGGCGCCGGAGCCACTG
GTACGTTTCGAAACCGATCCGGGGCAGCAGATGCAGGTCGACTGGATCGTGTTTCGACGTGGCAAGCTGCCGCTGTCGGCATTCGTTGCGACGCTCGGTT
ACAGTCGGGCGAGTTACGTCGAGTTCGTGACCGACGAACGATTGCCGACGCTGCTGGGATGCCATGAGCGCGCGTTCGACTTCTTCGGTGGGGTTCCTCG
CGAGGTCCTGTACGACAACGTCAAGACGGTGGTGATTGGCCGAGACGCCTATGGTCCCGGACTGCATCGCTATCAACCGGCCTTCCTCGACTTTGCGAAG
CACCACGGCTTCGTGCCGCGCCTGTGCAAGCCCTACCGAGCGAAGACCAAGGGGAAGGTTGAGCGCTTCAACGGATACCTGCGCCGCAGCTTCTACAATC
CGCTGGCAAGCCGGCTCGCTCAGGATCGCCTCTCGCTTGACGCGACCACGGCGAACGCCGAGGTCGTGCGCTGGCTGAGAGATGTCGCCAATGTGCGCAT
CCACGGCACGACTGGACAGTCGCCGCTCGAGCGTCTCCTGCTTGAGCAACCGAAGCTGCAGCAGTTGCCGGAGCCGTGGCGCGCGAACCTGCCGTCAGCT
TCCGTGCCGACGATGCCACCGCCGTCGCGGTTCGATGATACGCCGCTGCAGCATCAGCTGTCAGTGTATCAAGCCCTGCTGACGGAGGCACGTTGATGGA
CATCCAGCACGAACGTATCCTCGAAGCCTGTCGACAACTGCGGATCGACACGATGGGCGAGCAGTACAGCATGTTGGCAGCTCGCGCGGCAGCGAACCAA
CTCACGTTTGCCGACTTCTTCGAGGCGCTGTTGAAATCTGCGATGGACGCGAAGCACACGCGGACCAAATCCATGTTGCTGCGCACGGCCGGCTTCCCTG
TGCTGAAGACGCTGGAGGAGTTCGACTTTACGTTTGCTCACGGCGTTGCCAAGAAATCGGTGCTGGAGCTGTCGTCGATGGCCTTCGTCGAGCGCGCCGA
GAACATCGTGTTGCTCGGGCCGAGCGGCCTTGGCAAAACGCATCTGGCGATCGCACTCGGTTACATCGCAACGCAGATGGGGATCAAGACGCGCTTCATC
TCTGCGGCGGATCTCGTCATTGCCTTGGCCGCCGCTTCCCGGCAGGATCGATTGGCCGACTTCCTGAAGCGCAACATCATGAACCCACGGCTGTTGATCA
TTGATGAGGTCGGCTATCTGCCGCTCGGCCGGGACGATGCCAATCTGTTCTTCCAGGTGATCGCCAAGCGGTATGAAAAGGGTTCCGTGATCGTAACGAG
CAACTTGCCGTTCGGGCAATGGGACCAGACGTTCGCCGGAGATCAGACGCTCACGGCCGCGTTGCTCGACCGCCTCCTGCATCACAGCCATGTTCTACAG
ATCAAAGGCGAAAGCTACCGACTGAAGGACAAGCGAAAAGCCGGCGTGATCCGATCCCGTGCATCCGAAAGTGCTGAACAGCAACTGGAGATGCACGCGT
AAGTGATGATCAGCAACATCGTTTGACCCTGGGTCAGGCGAAACCGATGGCCAGCAACTGACGCTGACCCAGGGTCA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1011 bp | 336 aa | 86 | 1096 | + | No |
Chemistry : DDE
ORF sequence :
VEIRVLARQGLGIREISRELGLSRNTVRKYLRSCAEQDRAPRSGRTQKLDPFKTYLHDRVRAAHPAWLPATALLIEIRAMGYDGGLTRLRVYLRSLKSIK
APEPLVRFETDPGQQMQVDWIVFRRGKLPLSAFVATLGYSRASYVEFVTDERLPTLLGCHERAFDFFGGVPREVLYDNVKTVVIGRDAYGPGLHRYQPAF
LDFAKHHGFVPRLCKPYRAKTKGKVERFNGYLRRSFYNPLASRLAQDRLSLDATTANAEVVRWLRDVANVRIHGTTGQSPLERLLLEQPKLQQLPEPWRA
NLPSASVPTMPPPSRFDDTPLQHQLSVYQALLTEAR
APEPLVRFETDPGQQMQVDWIVFRRGKLPLSAFVATLGYSRASYVEFVTDERLPTLLGCHERAFDFFGGVPREVLYDNVKTVVIGRDAYGPGLHRYQPAF
LDFAKHHGFVPRLCKPYRAKTKGKVERFNGYLRRSFYNPLASRLAQDRLSLDATTANAEVVRWLRDVANVRIHGTTGQSPLERLLLEQPKLQQLPEPWRA
NLPSASVPTMPPPSRFDDTPLQHQLSVYQALLTEAR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 807 bp | 268 aa | 1096 | 1902 | + | No |
AG : IS21 helper
ORF sequence :
MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSMLLRTAGFPVLKTLEEFDFTFAHGVAKKSVLELSSMAFVER
AENIVLLGPSGLGKTHLAIALGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRNIMNPRLLIIDEVGYLPLGRDDANLFFQVIAKRYEKGSVIV
TSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGESYRLKDKRKAGVIRSRASESAEQQLEMHA
AENIVLLGPSGLGKTHLAIALGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRNIMNPRLLIIDEVGYLPLGRDDANLFFQVIAKRYEKGSVIV
TSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGESYRLKDKRKAGVIRSRASESAEQQLEMHA
Blast result :
Comments
ISRme20 is 69%(ORFA) and 76%(ORFB) aa similar to ISLpn12.
References
1] Rob Van Houdt (2014) Direct submission ISfinder
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Martinez,M., Goltsman,E., Pitluck,S., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Mergeay,M., Benotmane,M.A., Vallaeys,T., Michaux,A., Monchy,S., Dunn,J., McCorkle,S., Taghavi,S., van der Lelie,D. and Richardson,P. (2006) Direct submission GenBank
3] Janssen,P.J., Van Houdt,R., Moors,H., Monsieurs,P., Morin,N., Benotmane,R., Lapidus,A., McCorkle,S., Monchy,S., Taghavi,S., van der Lelie,N., Dunn,J., Leys,N. and Mergeay,M. (2010) Direct submission GenBank
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Martinez,M., Goltsman,E., Pitluck,S., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Mergeay,M., Benotmane,M.A., Vallaeys,T., Michaux,A., Monchy,S., Dunn,J., McCorkle,S., Taghavi,S., van der Lelie,D. and Richardson,P. (2006) Direct submission GenBank
3] Janssen,P.J., Van Houdt,R., Moors,H., Monsieurs,P., Morin,N., Benotmane,R., Lapidus,A., McCorkle,S., Monchy,S., Taghavi,S., van der Lelie,N., Dunn,J., Leys,N. and Mergeay,M. (2010) Direct submission GenBank