ISfinder Mirror Site

ISHvo19

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013966	ND	Haloferax volcanii	Haloferax volcanii DS2 plasmid pHV4

DNA section

IS Length : 1435 bp

Ends

Left end : AGAGGTGCAAGGAGGAAGCCCACGACTTCAGCCGTGGGAAGAATCCGACTCGAAACAAACCACGAGCGATAGCACCCCGACTCCCCCACGCAAGTCGTAG II struct. : Yes
Right end : GGGACGCTGAACCTGAACGGCGATTTCTGCGCCTCCGTCGATTCGACGGCAGAAGGGGAGTCCACTGACAAGCCCACGACTTCAGTCGTGGGTAACTGAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GACGAACGAG	TCAC	ACACCCGATC	tgac

DNA sequence

AGAGGTGCAAGGAGGAAGCCCACGACTTCAGCCGTGGGAAGAATCCGACTCGAAACAAACCACGAGCGATAGCACCCCGACTCCCCCACGCAAGTCGTAG
TTTTATAAAGAACTAATCTGATATGTGAAATACGAATGGAGGACGTGATTCGCACCGTCAAGGTCAAACTCGACGTACCCAACGAGCGGTGCGACGACCT
CCATCAGACAAAAAATCAGTTCCTCCACTGTGCGAACACTACTGCAGAGTGGGCGTGGAGACACCCGGACGACTACTGCGTGACCTCGAAACAGAAAGCC
GAGAAAGCCCTCTACGAGCAACTCCGCAACGAGACAGAGTTGACTGCAAACCTCGTTCAGAAAGGGATTCGGCGTGCTATCGAGGCCACGAAAAGCGGTG
TCGCCCGACTCAAGAAAGGCGACAACACGAGCCAACCGCACTTCGACGCGTGGAGCGTCGTCTACGACAAGCGTTCAGCGACGTTCCACCGCGACCACGT
TTCGCTCTCGACGGTGAACGGTCGCGTCAAGTGTGACTACGTGATTCCCGACAGCTCCGAGGGAACGCCGATTGGCGAGTACCTGTTGAATGAGGATTAC
GAGTTCCGAATGTCCACGTTGCAATACGACCGCGCCACAGAGTCGTTCTACCTCCACGCACGGATGCGCCGAACCACAGACGAACAAGAGCTGTCGATGA
CTCATTCTGACGCCAAGCACAGAACAGTCCTTGGTGTTGACCTGAACGTGGACGGCTCGCTCGCCGTGACGTCCACAGGCGCGTTCATCGGGAACGCCGA
CGAGATGAATCATCGACGCCGAGAGTTCGAGAAGACGCGTGGGTCGATGCAACAAACTGGAACGCGGTCGGCACACCTGTCGATTCAGTCGATGAAAAAC
CGCGGACACCGCTGGATGCAGGACGAACTACACCGAGCGTCTAACCAGATTCTCGAAGAAGCCCGCGACCACGACTGTACTCATATCTCATTCGAGAATT
TGACCGACATTCGTAAGCGGATGGCTGGCACCAAGCGGTTCCACGCATGGGCGTTCCGACGCCTGTACCAGTACACCGAATACAAAGCCGAAATGTTCGG
AATCGGGGTTGAACAGATAAGTCCTGCATACACGAGTCAGCGGTGTTCCAAGTGTGGATTTACGCATGAGGACAATCGGCGGTCGAAGCACCAGTTCGTG
TGTCAGAAGTGTGAGTACGAACTGAACGCGGAGTATAACGCGAGCAAAAACATCGCTCGCAAACTCTTGAAACGTCTCCACTCGGGGCAGAAGTCTTCGA
GTGGAGGCGCACCCTGTCAGTGTGCGCTACCGTCAGGGACGCTGAACCTGAACGGCGATTTCTGCGCCTCCGTCGATTCGACGGCAGAAGGGGAGTCCAC
TGACAAGCCCACGACTTCAGTCGTGGGTAACTGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1299 bp	432 aa	136	1434	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MEDVIRTVKVKLDVPNERCDDLHQTKNQFLHCANTTAEWAWRHPDDYCVTSKQKAEKALYEQLRNETELTANLVQKGIRRAIEATKSGVARLKKGDNTSQ
PHFDAWSVVYDKRSATFHRDHVSLSTVNGRVKCDYVIPDSSEGTPIGEYLLNEDYEFRMSTLQYDRATESFYLHARMRRTTDEQELSMTHSDAKHRTVLG
VDLNVDGSLAVTSTGAFIGNADEMNHRRREFEKTRGSMQQTGTRSAHLSIQSMKNRGHRWMQDELHRASNQILEEARDHDCTHISFENLTDIRKRMAGTK
RFHAWAFRRLYQYTEYKAEMFGIGVEQISPAYTSQRCSKCGFTHEDNRRSKHQFVCQKCEYELNAEYNASKNIARKLLKRLHSGQKSSSGGAPCQCALPS
GTLNLNGDFCASVDSTAEGESTDKPTTSVVGN

Blast result :

Comments

ISHvo19 is 71% aa similar to ISNpe12.There is an empty site in Halobacterium sp. DL1 plasmid (CP007061).

References

1] ISfinder annotation (2014)
2] Madupu,R.M., Hartman,A.L., Norais,C., Badger,J.A., Delma,S., Haldenby,S., Khouri,H., Ren,Q., Lowe,T., Robinson,J., Furlow,J.M., Pohlschroder,M., Pfeiffer,T., Allers,T., Daniels,C. and Eisen,J.A. (2010) Direct submission GenBank. J. Craig Venter Institute.