ISfinder Mirror Site

ISHvo18

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013964	ND	Haloferax volcanii	Haloferax volcanii DS2 plasmid pHV3

DNA section

IS Length : 1892 bp

Ends

Left end : ACATAGTGCGAGCGCATACCCGCGTCTTCAGGCGCGGGTCAAACGGACAATAGCGTACACAACCACCGACGGTAGTACGGCTGGATTTCCCACCGTGTAA II struct. : Yes
Right end : CCATGGTGGGATTCCCGCGTTGACGAGACGCTCTGCGTCTCGTTCGCACACCAGAACGCTGTGCGTTCTGGGGACGACTTTAGGCGCGGGAGGTGGTCAA II struct. : No
Comments : Comparing to ISH35, there is an insertion of 57 nts (TGACGAGACGCTCTGCGTCTCGTTCGCACACCAGAACGCTGTGCGTTCTGGGGACGA) inside the rigth end of the sequence. This inactive the RE.

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CGAGTCCGCC	TGAT	TAGGCCGCGC

DNA sequence

ACATAGTGCGAGCGCATACCCGCGTCTTCAGGCGCGGGTCAAACGGACAATAGCGTACACAACCACCGACGGTAGTACGGCTGGATTTCCCACCGTGTAA
GGTGGTTTTAAGACACACGCTACGTATAGTGTGTAGTACGGATGAAGGCCACACGGCACGCAACGTACAACCTCAACTACCACATAGTGTGGATACCGAA
GTACCGTCGGTCGGTACTCGTCGGTGACGTTGCAACCCGTGTGCGAGACATCCTCCACGAAATCGCTGACGAGAAGGGGTTGGAGATTATCGACCTCACT
GTCCAACCCGACCACATCCACCTGTTCGTCAGTAGCCCGCCGAAGCACGCTCCGTCACTTCTCGCCAACTGGTTCAAAGGCATCAGTTCGCGGAAGTACA
ACCACCGACACGCCGACCACGACAGTGAGAAAATTAAGTGGGTGCGAGGCTACTACGCGGGGACAGCAGGACACGTTTCGAGTGAGACTGTCGAGAAGTA
CATCCAGCGTCACGAGGAGGACGAACAGTGACCGAACTCACCGAGACGCTGGAACTGAAGCTTGTGGAACCGAACACCCACAAGCATCGGAAACTCTGTG
AGACGGAACAGGCGTACCAAGACGCCCTTGAATCCGCGTTCAACGCTAACTGCACCACACAGTCGGCGGCGAACGACGTGGTAGTCAACTACGACCTGAG
CGGCTACGCCAAGAACGCGCTCAAGAAGTACGTCCCACAACTCTGTGGCGGAAGCTACGACGCCAAAGAGCTTCACGACGACCACCCCGTTCGGTTCACG
AACGAAGGCCCGAAGCTCGACCACAAGCCACAGAACGCTATCGAGTGGTACGTCAAAATCCCGCACCACGACGACTACAACCTCTGGTTGCCCGCGCAAC
CGAATCCCGAACAGCGAGAATGGTTGGAAGCGTTGCACGCAGGAGACGCACAGATGGGCGAATGTCGGTTGTTCGACCGTGACGGCGAGTGGTACTTTCA
CATCGTCGCCACGCGGGACGTGGAGGAACGAGAGACCAGTTCGGTGGAAACGCCGATTGGGGTAGACATCGGGGAAGCCTCTTTGTTAACGGTGTGTCAC
CGTGACGAGCGCGGCTCCCCGACTGCACCCAACCTCTGGAACGACGAAGGCATGCGAGTACGGCAACTCCGTGAGACGTACTTCACGGCGACTCGACGCC
TTCAAAAACGCGGAAGCGAACGTATCGCTGAATCCTACGGCGACGAGTTGTGGCGACACATCGACCACATCCTTCACACGGTCACGTCGGAGGTCGTCTC
CTACGCCGACCGATTCGAGAATCCCGTGTTGGTTCTGGAAGACTTGACGCACATCCGTGAGAACATGGACTACGGCGCGTTCATGAATCGACGGCTTCAC
GGATGGGGCTTCGCCAAGATGCACGCTCAGATTCGCTACAAGGCGTCTGAGAAAGGGATTCGTGTGGAGACAGTGAATCCCGCGTACACCTCGAAAACGT
GCCACTGTTGCGGAGAGGCGGGCTACCGTCCGAAGCAGGCGACGTTCAAGTGTTCCAACTCGGCGTGTTGGGTTTCGGAGTACCAAGCAGACGTTAACGC
CGCGCTCAATATTGCAGACCGCTACCTCAGTGGAGAGAGTCATTCAAGAGAACACACGGATGGCGATGACTCGGCTGAGGAAGGGGGACGTTTGACCGTC
CCACAAGACAGCCAAGCCGATGCTGACACCCAGCAAGAGACGCTTGGAACGTATGCGTCTTGAAACCTTAGGGCCGCACTCGGCCTGAAATCCCATGGTG
GGATTCCCGCGTTGACGAGACGCTCTGCGTCTCGTTCGCACACCAGAACGCTGTGCGTTCTGGGGACGACTTTAGGCGCGGGAGGTGGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	142	531	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKATRHATYNLNYHIVWIPKYRRSVLVGDVATRVRDILHEIADEKGLEIIDLTVQPDHIHLFVSSPPKHAPSLLANWFKGISSRKYNHRHADHDSEKIKW
VRGYYAGTAGHVSSETVEKYIQRHEEDEQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1236 bp	411 aa	528	1763	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTELTETLELKLVEPNTHKHRKLCETEQAYQDALESAFNANCTTQSAANDVVVNYDLSGYAKNALKKYVPQLCGGSYDAKELHDDHPVRFTNEGPKLDHK
PQNAIEWYVKIPHHDDYNLWLPAQPNPEQREWLEALHAGDAQMGECRLFDRDGEWYFHIVATRDVEERETSSVETPIGVDIGEASLLTVCHRDERGSPTA
PNLWNDEGMRVRQLRETYFTATRRLQKRGSERIAESYGDELWRHIDHILHTVTSEVVSYADRFENPVLVLEDLTHIRENMDYGAFMNRRLHGWGFAKMHA
QIRYKASEKGIRVETVNPAYTSKTCHCCGEAGYRPKQATFKCSNSACWVSEYQADVNAALNIADRYLSGESHSREHTDGDDSAEEGGRLTVPQDSQADAD
TQQETLGTYAS

Blast result :

Comments

ISHvo18 is 91% (TnpA : the transposase) and 85% (TnpB) aa similar to ISNpe10.

References

1] ISfinder annotation (2014)
2] Hartman,A.L., Norais,C., Badger,J.H., Delmas,S., Haldenby,S., Madupu,R., Robinson,J., Khouri,H., Ren,Q., Lowe,T.M., Maupin-Furlow,J., Pohlschroder,M., Daniels,C., Pfeiffer,F., Allers,T. and Eisen,J.A. (2010) PLoS ONE 5 (3), E9605.