ISHhu1

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LN831302 ND Halobacterium hubeiense
Halobacterium hubeiense plasmid pSTJ001
Halobacterium hubeiense
DNA section
IS Length : 1835 bp

Ends


Left end : GGAAAATTCAAGGCGAAAGCCTCGCCCTTCAGGGCGGGGATGAAGCCGACCAACAGTATTCAACCGCCGTCGATGGCATAGACGCGGTTCCAACACAATT II struct. : Yes
Right end : TCCTAAAGAAGTGCGCACAAACCCGCAAGTTGCCTCCGTGGGTCGGTAGCCGAACCCCCAACGGAGGAATCCTCGCGCTTTAGCGCGGGGAGGATGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TCAGGGGGCGTATTACTGGGTGTCGTTCTCAA
AGACTTTAATCGTTACTCACTGACAAATTCAA

DNA sequence

GGAAAATTCAAGGCGAAAGCCTCGCCCTTCAGGGCGGGGATGAAGCCGACCAACAGTATTCAACCGCCGTCGATGGCATAGACGCGGTTCCAACACAATT
TTTAACCGGTCCGACCATGATAGTATACATAGATGGTAAAGAGTACCCGTCACGCGAAATACGAACTCTATTACCACATAGTGTTCGTGCCGAAGTATCG
GCGTTCGCCCCTGACGGGGAAGACGAAGGACCGTCTCGAAACCATCTTCACGGAAATCTGTGAGGACAAAGACCTCGAACTGGCCGAGTCCGAGGTCATG
CCCGACCACGTACACCTGTTCATCGGGAGTCCACCGAAGAACGCCCCGTCACTCATCGTCAACTGGGTCAAGGGCATCTCGGCGCGGAAGTATAACCAGC
GATACGACGACCGCGTGAAGTGGACTCGTTCGTACTACGTCGGTACGGCGGGTAGTTCCTCGAAGGGCGCTGTCGAACAGTACATCGCTGAACAGGAAGG
TGGTGACGAATGAAGCGCGTCAACACCTTCGAGGTCGTGCCACAGACCGAGAACGACAAAGAGTGCCTTCTACGGCTACTCGACGCCTCCGCCTCCCTGT
GGAACGAACTCACCTACGAACGCCGTCAGAACTACTTCGGTGACGGCGACGTGTGGGACACCTCCGAGTACCGAGGACGCTACAACGGTGTCGTCGGAAG
CGCGACCGTTCAACAGGTCACGCGCAAGAACAGCGAAGCGTGGCGGTCGTTCTTCGCGCTCAAAGAGAACGGAGAGCATGCGAACCCACCGTCGTACTGG
GGCAACGAGGAGGATGGACGAGAACTCCGTACCTACATCCGAAACAACCAGTACACGATTCAGTGGGGCAAGCGTAGCCGTCTCGAACTCCCCGTTGGGC
AAGAGTTGAAAGACGAATACGGACTCGGCTACCACGAACGCCTCCGTCTCGAAGTCCGAGGCAACCCGAAGTGGGACGGCGAACAGGGTCGTCTGGAACT
TGAGTACGACGGGGTGAGCGACACGTTCAGGGCTTTCCAACCAGTCACAGTCCCTGATTCTCGACTGGATTCACCACTGGCTTCGGAAGAAGCCGCCCTC
GACGTTGGCGCAAACAACCTCGTCGCCTGTTCTACGACTACTGGGAACCAGTACCTCTACGACGGTCGGGAGTTGTTCGGACGGTTCCGTGAGACGACTG
ACGAAATCGCCCGCCTGCAGTCGAAACTCCGCGAGGGACGCTACAGTTCCAAGCGGATTCGACGGCTGTATCGACAGCGGACGAAGCGCCGTGACCACGC
ACAGAACGCGCTGGTGCGCGACCTCGTTGAACGGCTGTACGACGAGGGCGTGGCGACGGTGTACGTGGGCGATTTGACCGACGTGCTGGAAACGCACTGG
TCGGTCAGGGTGAACGAGAAGACGCACAACTTCTGGGCGTTCAAGAAGTTCATCCACCGTCTCGCGTGTGTCTGTGAGGAATACGGCATCAGCCTCGAAG
TCGAGTCGGAAGCGTGGACAAGTCAGACGTGTCCCGAGTGTGGCGACCACGAGGAGACGGTTCGCCATGAGGATACGCTGACGTGTCCGTGTGGGTTCGA
GGGACACGCAGACCTCACGGCGTCAGAGACGTTCCTTCGGGAAAACAGCGGTACGGAAGTCAGGCCGATGGCACGGCCCGTGCGATTCGAGTGGGACGAC
CACGATTGGTCGGGGAAACCACACCCTCACGAAAGTCCTAAAGAAGTGCGCACAAACCCGCAAGTTGCCTCCGTGGGTCGGTAGCCGAACCCCCAACGGA
GGAATCCTCGCGCTTTAGCGCGGGGAGGATGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
381 bp126 aa133513+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MVKSTRHAKYELYYHIVFVPKYRRSPLTGKTKDRLETIFTEICEDKDLELAESEVMPDHVHLFIGSPPKNAPSLIVNWVKGISARKYNQRYDDRVKWTRS
YYVGTAGSSSKGAVEQYIAEQEGGDE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1275 bp424 aa5101784+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRVNTFEVVPQTENDKECLLRLLDASASLWNELTYERRQNYFGDGDVWDTSEYRGRYNGVVGSATVQQVTRKNSEAWRSFFALKENGEHANPPSYWGNE
EDGRELRTYIRNNQYTIQWGKRSRLELPVGQELKDEYGLGYHERLRLEVRGNPKWDGEQGRLELEYDGVSDTFRAFQPVTVPDSRLDSPLASEEAALDVG
ANNLVACSTTTGNQYLYDGRELFGRFRETTDEIARLQSKLREGRYSSKRIRRLYRQRTKRRDHAQNALVRDLVERLYDEGVATVYVGDLTDVLETHWSVR
VNEKTHNFWAFKKFIHRLACVCEEYGISLEVESEAWTSQTCPECGDHEETVRHEDTLTCPCGFEGHADLTASETFLRENSGTEVRPMARPVRFEWDDHDW
SGKPHPHESPKEVRTNPQVASVGR

 

Blast result :
Comments
ISHhu1 is 67% (TnpA : the transposase) aa similar to ISNpe10 and 80% (TnpB) aa similar to ISNpe11.
References
1] F. Pfeiffer (2013) Direct submission.
2] Jaakkola S.T., Pfeiffer F., Ravantti J.J., Guo Q., Liu Y., Chen X., Ma H., Yang C., Oksanen H.M., Bamford D.H. (2015) Environ Microbiol. doi: 10.1111/1462-2920.13130