ISHmu5

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001688 ND Halomicrobium mukohataei
Halomicrobium mukohataei DSM 12286
DNA section
IS Length : 1847 bp

Ends


Left end : CTTGATTCAGCGAGAGAATCCCTCCGTTCACGGCGGGCGTGAATCGTGTAAGCTCGGTGCGACAGTCCACTCGGTTCTGTCCGACCGAATACCCATTGGC II struct. : No
Right end : CGGATGACGCTCGGCGAGGTCGGGTCGGAACCCTCTGCCGGTAGTTAGCCGGCATTCCCATGCAGGGAAGCCGCGCCGTTCACGGCGCGGAGGATGTCAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TACATCGAGTAGCTATTCGAGTGCTTCT

DNA sequence

CTTGATTCAGCGAGAGAATCCCTCCGTTCACGGCGGGCGTGAATCGTGTAAGCTCGGTGCGACAGTCCACTCGGTTCTGTCCGACCGAATACCCATTGGC
ACAAGAGTTATTGAAATTGGGTGTCTTATATTTGGTAAGGCCAAATGGAGTCTCACCTGCAATCTGGGTCGCACACGGTCTACGCGCTCCAATATCACTT
CGTGACCGTCACGAAGTACCGCGCCGACATCCTCACCGATGAGCGGCTGGAGCGCGTGGCTGAAGTCGCACACGAGATTGCAGAGGACTTCGAGGCCGAC
ATCAAGAACGTGGACGGCGGTACTGACCACGTTCACATCCTGTTCACGACCAAGCCAACCACAGACCTCACGAAGTTCATCAACTCGCTCAAGGGCGTCA
CATCCCGCCGGATTCGGCAGGAGTACCCCGAGGTGAAACAAACGCTCGAAGATGCGTTCTGGCAACCGGGATATTTCCTCGCCACGACCGGTCAAGTGAG
CATCGACGTGCTGATGGACTACGTGGAAAACCAGTAGTATGACCGCGACAACCACGAAAACGCTGGAGGCCACGCTCGCCCCGCCGACAGCCCACAAAGA
GCGTAAACTGTGCGACCTGCTCGACACCTACCGGGCAGGACTCCACGAGGCGTTCGGGGCCGGGTGCGAGACGATGACCGCCACCAGCGACGTGGTAACG
CCCTACGACCTGCCGTATCAGGCGAAGGCGGCCCTGTGTAACTACGTTCCGCAACTGCACGGCACCTACGATGCACAGGAGCTGGACGACGACCACCCAA
TTCGACTCACCAACCAAGCCGCCGAGTTTGACTACTCATCGGAACGGGACTACGAGTTTACGTGGTGGGTGCCACAGCCCGGTCGCGGGACGAACTTCTG
GATACCGCTTCGTATCAACCCCGCTCAAGAAGAACTGTGGCACGACCTCGTGGACGGTGAGGCGTCAGCGGGACAGCTCCGTCTGCAACGTCACCACACG
TCGTGGACGCTTCACGTCACCGTCGAGTTCCCGGTCGAAGAACCAGACTACGAACCGACAGACGACGATGTAACACCAGTCGGCTTCGACATTGGCGAAG
CGCACCTGCTCGCGGGCTGTGCCTGCGAGCAGGACACTCCGACCGACCCACTACTCATCAACGGTGGCCGCGCTCGTCACCTCCGCAAGGAGATGTACAC
GACCCTGAAGCGACTCCAAGAGCGCGAGGCCACCGCGTGGCGGATTGACGAACGGTTCGACCACTACCAGAACGCACTCACGGACATCATCGAGAAGGCG
TCTCGACAGGCAAACGAGTACGCAAGCCGCTTCGAGAAGCCTGTGGTCGTTCTGGAAGACCTCTCGTACATCCGCGAAGAACTCGATTACGGCGAGTGGA
TGAATCGACGCCTCCACGCATGGGCGTTTGCTCGCTTACAGGAGCGCATCGAGGACAAAGCACGAGAGGCCGGGCTTCCGGTCGAATACATCCGCCCGGA
GTACACGAGCCAGACGTGCCACGAGTGCGGCCACGTCGGACACCGGAACGGCGACGAGTTCTGGTGTACGAACGACGAGTGTTGGGTCACGGAGTATCAC
GCAGACATTAACGCGGCGGTCAACATTGCTGGCCGCCACGACCCGTGGGGTGAGAGCCTGCCGCTGAAACCGGCAAGCGATGACATCTCACGGGATGGGA
GCGCCTGTGACAGCGCCGCGATCCCCACCGAGCAGAGCCAACCACGGCGGATGACGCTCGGCGAGGTCGGGTCGGAACCCTCTGCCGGTAGTTAGCCGGC
ATTCCCATGCAGGGAAGCCGCGCCGTTCACGGCGCGGAGGATGTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
393 bp130 aa145537+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MESHLQSGSHTVYALQYHFVTVTKYRADILTDERLERVAEVAHEIAEDFEADIKNVDGGTDHVHILFTTKPTTDLTKFINSLKGVTSRRIRQEYPEVKQT
LEDAFWQPGYFLATTGQVSIDVLMDYVENQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1257 bp418 aa5391795+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTATTTKTLEATLAPPTAHKERKLCDLLDTYRAGLHEAFGAGCETMTATSDVVTPYDLPYQAKAALCNYVPQLHGTYDAQELDDDHPIRLTNQAAEFDYS
SERDYEFTWWVPQPGRGTNFWIPLRINPAQEELWHDLVDGEASAGQLRLQRHHTSWTLHVTVEFPVEEPDYEPTDDDVTPVGFDIGEAHLLAGCACEQDT
PTDPLLINGGRARHLRKEMYTTLKRLQEREATAWRIDERFDHYQNALTDIIEKASRQANEYASRFEKPVVVLEDLSYIREELDYGEWMNRRLHAWAFARL
QERIEDKAREAGLPVEYIRPEYTSQTCHECGHVGHRNGDEFWCTNDECWVTEYHADINAAVNIAGRHDPWGESLPLKPASDDISRDGSACDSAAIPTEQS
QPRRMTLGEVGSEPSAGS

 

Blast result :
Comments
ISHmu5 is 99% (TnpA : the transposase) and 96% (TnpB) aa similar to ISNph5.
References
1] Pfeiffer, F. (2013) Direct submission
2] Tindall,B.J., Schneider,S., Lapidus,A., Copeland,A., Glavina Del Rio,T., Nolan,M., Lucas,S., Chen,F., Tice,H., Cheng,J.F., Saunders,E., Bruce,D., Goodwin,L., Pitluck,S., Mikhailova,N., Pati,A., Ivanova,N., Mavrommatis,K., Chen,A., Palaniappan,K., Chain,P., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Brettin,T., Han,C., Rohde,M., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Klenk,H.P., Kyrpides,N.C. and Detter,J.C. (2009) Stand Genomic Sci 1 (3), 270-277