ISHmu3

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001688 ND Halomicrobium mukohataei
Halomicrobium mukohataei DSM 12286
DNA section
IS Length : 2029 bp

Ends


Left end : AGGCGGAGCAGGCCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGGCCGTACACTCCGCTAAACGTGTTCCGTTCGCTCGATATACTGCTCAATCGTGT II struct. : Yes
Right end : GTGACCCATTCGACCGTTCGAGGCTATCAGGCCGATGGTCGGATGGGTGTGTCTGATTAAACCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTACACCGGCAGTCTACAAGAATCTTCTCTCAC

DNA sequence

AGGCGGAGCAGGCCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGGCCGTACACTCCGCTAAACGTGTTCCGTTCGCTCGATATACTGCTCAATCGTGT
CGGTCGAAACATCGCCTGCCGTCCCCACGTAGTACGATTCCTCCCAAAATCCACCGCCCCACAGGTACTCCTCCAAGAACGGTTCGTGCTGTTCCCACAT
CTCCCGTGCCGTGATGCTCTTGATTGTCCGTACAATCTCGCTCGGCCCGTACTTCGGGTGAGCTGATAGGAACAAGTGTACGTGATCGGGCGAAATATGT
AGCGATAGTATCTCGTAACCGTACTCGTCGCACACATCGCGGAAACTCGCTTCCAGCGAATCCTCGATTGGTTCGAGAATCGTGTAGCGATACTGAGCGG
GATCTTCGATCCCGCGAGGTACGCAAAGCTTCGCTTTGCTTGACTTCGGACACCACACGAAATGGTAGTTGACGTTGTACACCGTGTGATTCGACCGCTT
CTCACCCATATCGAACCTACTCCACCAGCACAGTTAAGTATATCCAAAGGATATACGCAGGTATGGCGAATCGCTTTGAGATCGACGGCGAAGAAGTTCT
CGACGGTGAAGTCAAGCCGTTCGGGAACAGCGCCCATGTCACCGTCCCTAAACGCTGGCGTGGAGCAGATGTGAAAGTCGTCCGAACTTCAGAACCCACC
GAACAAGACGAAGAATGACCACGGCACAGGCTCTCGTCAAGACGCTAGACTTCCAACTCGACATCCAGAGTGACAACGAGAGCCTGTTGTACGACGCCAC
GCTTGAAGCCCGCTCGGTGTACAACGAAACCATCCGCCTCGCGAAAGAAGGCGTGGACTGGGACACCACTCCTGACTGTGTGGCTGATGACGCTGACCTC
GTGAAGAACACGACACAGCGCGTCGTCGCCAAGGCGCTCGGCGCGATGGAGAACTACTACGAGTACGACGACTTCAGCCAACCCAGCCACACCAAGGACG
GCGCGTACCCGCTTCGTGCGAACTATGAGGAGGGGTACAACCTGTCGCTCACCGACGATGGCGGCGTGGCGTTCCGTATCAGCACGGAGCCGTACAAGCA
CGTCAAGGGCTTTCTCACCGGGAGTGACGCCCACCTCGACATTCTCAAGACCGCGCTCACGAGCGACGAGTGGAAAATTGGGACGGCGGAAGCCCTGTTC
CACAACGACGACGCCGAGTTGCACGTCAACGTCACCAACACCGAGCAGACCGTTCGGGACAAGGAAGACTCACGGACTGTCGTGGGTGTGGACGTGAACG
AGGACAACGTGGCTCTCACCGCACTTTCCGGAGATGGCGTCAAGCGCACGTTGGTTATTGACTTCCCCGAAATCAAGTTCGAGCGCCACCGCTACTTCAC
GATGCGAAAGCGCGTCCAGAACGCGGGGAAGGACAGCATCCACGACACGCTCGAAGGACGCGAAGAACGGTTTGTCCGCGACCGCCTCCACAAGGTGTCT
CGACACATCGTAGAGTGGAGCCAGCAGTTCGAGAAGTCGTGCATTGTCTTTGAAGACCTCAAAGAGATGCGCGACAGTATCGACTACGGGACGCGGATGA
ACCGACGCTTGCACCACCTCCCGTTCCGCGCCCTCCAATACTACACGTCGTACAAGGCGTCGTTTGAGGGTATCCCGACTGCGTGGATCAATCCCGAATA
TACGAGTCAGCGGTGTCCGATGTGTGGGCATACAGAGCGAGCGAATCGAAACAAAAAGCGGTTCGAGTGCAAGTCCTGCGAGCATCAAGACCACAGCGAC
CGTAGTGCAAGCGTCAACATCGCCGTGAAAGGCGTGAAGAAACTCGATTGGAATGTGCCTGCTCTCAACAGCCTTCCCGTTGTTCGGAAGGTGCGACGGC
AGGCATCGGGGGCCGTGGACGCCCCGACCGTGACCCATTCGACCGTTCGAGGCTATCAGGCCGATGGTCGGATGGGTGTGTCTGATTAAACCACGGGAAG
CCTCGGGGCTTGACCCCGAGGCGGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
450 bp149 aa50960-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEKRSNHTVYNVNYHFVWCPKSSKAKLCVPRGIEDPAQYRYTILEPIEDSLEASFRDVCDEYGYEILSLHISPDHVHLFLSAHPKYGPSEIVRTIKSIT
AREMWEQHEPFLEEYLWGGGFWEESYYVGTAGDVSTDTIEQYIERTEHV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1275 bp424 aa7151989+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTTAQALVKTLDFQLDIQSDNESLLYDATLEARSVYNETIRLAKEGVDWDTTPDCVADDADLVKNTTQRVVAKALGAMENYYEYDDFSQPSHTKDGAYPL
RANYEEGYNLSLTDDGGVAFRISTEPYKHVKGFLTGSDAHLDILKTALTSDEWKIGTAEALFHNDDAELHVNVTNTEQTVRDKEDSRTVVGVDVNEDNVA
LTALSGDGVKRTLVIDFPEIKFERHRYFTMRKRVQNAGKDSIHDTLEGREERFVRDRLHKVSRHIVEWSQQFEKSCIVFEDLKEMRDSIDYGTRMNRRLH
HLPFRALQYYTSYKASFEGIPTAWINPEYTSQRCPMCGHTERANRNKKRFECKSCEHQDHSDRSASVNIAVKGVKKLDWNVPALNSLPVVRKVRRQASGA
VDAPTVTHSTVRGYQADGRMGVSD

 

Blast result :
Comments
ISHmu3 is 90% (TnpA : the transposase) aa similar to ISNph7 and 95% (TnpB) aa similar to ISHma7.
References
1] Pfeiffer, F. (2013) Direct submission
2] Tindall,B.J., Schneider,S., Lapidus,A., Copeland,A., Glavina Del Rio,T., Nolan,M., Lucas,S., Chen,F., Tice,H., Cheng,J.F., Saunders,E., Bruce,D., Goodwin,L., Pitluck,S., Mikhailova,N., Pati,A., Ivanova,N., Mavrommatis,K., Chen,A., Palaniappan,K., Chain,P., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Brettin,T., Han,C., Rohde,M., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Klenk,H.P., Kyrpides,N.C. and Detter,J.C. (2009) Stand Genomic Sci 1 (3), 270-277