ISH36

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AM774417 ND Halobacterium salinarum
Halobacterium salinarum R1 (DSM 671) plasmid pHS2
DNA section
IS Length : 1978 bp

Ends


Left end : CGGCGGAGCAGGCCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGGCCGTAAGCCCCGTTAAACGTGTTCCGTGCGCTCGATATACTGCTCAATCGTGT II struct. : Yes
Right end : GTGACCCAATCGACCGTTCGAGGCTATCAGGCCGATGGTCGGATGGGAGTGTCCGACTAACCCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TCGGCGACACCGTCTACAAACAGAATCACtcac

DNA sequence

CGGCGGAGCAGGCCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGGCCGTAAGCCCCGTTAAACGTGTTCCGTGCGCTCGATATACTGCTCAATCGTGT
CGGTCGAAACATCACCTGCCGTCCCCACGTAGTACGATTCCTCCCAGAACCCACCGCCCCAAAGATACTCCTCCAAGTACGACTCGTGCTGTTCCCACAT
CTCCCGCGCCGTGATGCTCTTGACCGTCCGCACAATCTCGCCCGGCGCGTGCTTCGGATGGGTGGAGAGGAAAAAATGTACGTGGTCGGGTGAGATGTGG
AGCGACAGTATCTCGTAGCCGTACTCGTCGCACACATCCCGAAAACTCGCCTCCAGCGAATCCTCGATTGGTTCGAGAATGGCGTGGCGATACTTCGGAC
ACCACACGAAGTGATAGTTGACGTTGTACACCGTGTGATTCGACCGCTTCTCGCCCATATCGAACCCACCCCACCAGCACAGTTAAGTATATCCAAAGGA
TATACGCAGGTATGACGAATCGCTTTGAAATCGACGGCGAGGAAGTTCTCGACGGCGAGGTCAAGCCGTTCGGGAACAGTGCCCACGTCACCGTTCCCAA
ACGCTGGCGTGGTGCGGACGTAAAAGTCGTCCGAACCTCAGAACCCACCGAACAAGACGAAGAATGACCGACGCACAGGCTCTTGTCAAGACGCTGGACT
TCCAACTCGACATCCAGAGTGACAACGAGAGCCTGCTGTACGACGCCACGCTCGAAGCCCGCTCGGTGTACAACGAAACCATCCGTCTCGCCAAAGAGGG
TGTGGACTGGGACGCCATCCCCGACCGTGTGGCCGACGATGCCAACCTCGTGAAGAACACGACTCAGCGCGTCGTCGCCAAAGCACTCGGCGCGATGGAG
AACTACTACGAGTACGACGACTTCGGCCAACCCAGTCACACCAAGGACGGCGCGTACCCGCTTCGTGCGAACTACGAGGAAGGGTACAACCTGTCGCTCA
CCGACAACGGTGACGTGGCGTTCCGCATCAGCGCGAAGCCCTACAAACACGTCAAGGGCGTCCTCAAAGGGAGCGACGCTCACCTCGACATTCTCAAGAC
CGCTCTCACGAGTGACGAGTGGAAGATTGGGACGGCGGAAGCCCTGTTCCACAACGACACCCCCGAGTTGCACGTCAACGTTACCAACACCGAGCAGACC
GTTCGAGACAAGCAGTACTCACGGACGGTCGTCGGTGTGGACGTGAACGAAGACAACGTGGCTCTCACCGCTCTTTCCGAGGATGGCATTGAGGACTCGT
TGGTTATCGACTTCCCCGAAATCAAGTTTGAACGTCATCGCTACTTCACGATGCGGAAGCGCGTGCAGAACGCGGGGAAGAACAGCATCCACGACACGCT
GGAAGGGCGTGAGGAACGGTTCGTCCGCGACAGACTCCACAAGGTGAGTCGTCACATCGTGGAGTGGAGCCGTCAGTTCGAGAAACCGTGCATCGTCTTT
GAAGACCTCAAAGAGATGCGCGACAGTATCGACTACGGCACGCGAATGAACCGACGCTTGCACCACCTCCCGTTCCGCGCCCTCCAGTTCTGTACGTCGT
ACAAGGCCGCGTTCGAGGGCATTCCGACCGCGTGGATTAACCCCGAGTACACCAGCCAGCGGTGTCCGCTCTGCGGACACACGGAACGTGCGAATCGGAA
CAAGAAGCGGTTCAAGTGTCGGGACTGCGGGCATCAAGACCACAGCGACCGTGGTGCAAGCGTCAATATCGCTGTAAAAGGCGGCAAGAAACTCGATTGG
AATGTGCCTGCTCTCAACAGCCTTCCCACCGTGCGGAAGGTGCGACGGCGGGCATCGGGGGCCGTGGACGCCCCGACCGTGACCCAATCGACCGTTCGAG
GCTATCAGGCCGATGGTCGGATGGGAGTGTCCGACTAACCCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa45860-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEKRSNHTVYNVNYHFVWCPKYRHAILEPIEDSLEASFRDVCDEYGYEILSLHISPDHVHFFLSTHPKHAPGEIVRTVKSITAREMWEQHESYLEEYLW
GGGFWEESYYVGTAGDVSTDTIEQYIERTEHV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1275 bp424 aa6641938+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTDAQALVKTLDFQLDIQSDNESLLYDATLEARSVYNETIRLAKEGVDWDAIPDRVADDANLVKNTTQRVVAKALGAMENYYEYDDFGQPSHTKDGAYPL
RANYEEGYNLSLTDNGDVAFRISAKPYKHVKGVLKGSDAHLDILKTALTSDEWKIGTAEALFHNDTPELHVNVTNTEQTVRDKQYSRTVVGVDVNEDNVA
LTALSEDGIEDSLVIDFPEIKFERHRYFTMRKRVQNAGKNSIHDTLEGREERFVRDRLHKVSRHIVEWSRQFEKPCIVFEDLKEMRDSIDYGTRMNRRLH
HLPFRALQFCTSYKAAFEGIPTAWINPEYTSQRCPLCGHTERANRNKKRFKCRDCGHQDHSDRGASVNIAVKGGKKLDWNVPALNSLPTVRKVRRRASGA
VDAPTVTQSTVRGYQADGRMGVSD

 

Blast result :
Comments
ISH36 is 96% (TnpA : the transposase) and 97% (TnpB) aa similar to ISHma7.
References
1] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91: 335-346.