ISH34

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AM774415 ND Halobacterium salinarum
Halobacterium salinarum R1 (DSM 671)
DNA section
IS Length : 1853 bp

Ends


Left end : ACAACCTGCGAGGGCGAGTTCCCCGACCCACCGGGGTCGGGGGTGAAGCCCGACACTCGACCTAATGTTCTGTCTGCTCGATATACCGTTCAACCACTTC II struct. : Yes
Right end : GCCGCCCAACCTCATCCCGTTCCCTCGCGGAACAGGGAGTGTTAGCGCACGGCTGAGGGAACTACAAAAGCCTCGGGCCATCGTGCCCGAGGCTGTTTAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GCATCACGCACTTCTAATCCGTTCTCGCGTTAC

DNA sequence

ACAACCTGCGAGGGCGAGTTCCCCGACCCACCGGGGTCGGGGGTGAAGCCCGACACTCGACCTAATGTTCTGTCTGCTCGATATACCGTTCAACCACTTC
TTCTGACACCTCACCCGTCGTCCCCACGTAGTACCCAACCTTCCAGAACCCACCACCCCAGAAATACGACTCCCGAATCTCAGGATACCTTTCCAGCAAG
TGCTTGCCCGAATACGACTTGAACTGTCGAGCGATGTCGGCAGGACTGTGCTTCGGGTCGCACTGGACGAACAGGTGAACGTGGTCGTCTGCTATCTCCA
ACGCCAGAATCTCGTGCCCGAAGTGGTCGGCAGTCTCCGAAAACAACTCCCGCACATCGTCTTCAACCACGTTGAGAACCGGGTGGCGGTACTTGGGACA
CCACACAAAGTGATACTTGCAGGAACTAACCGAATGCGCATGACTGCGGTACTCCTCCATCCCTAATCCCTACATTTATGACAGTATGGAACGATTATCA
AGGTATGGGTGAAGAAGCCACGAAAACCATTCAGACGCGCCTTCAAATAGCGTCTGGTGAACGGTCGTGGCTTCACGACGCCCGCCTCGCCTCACGCGAG
ATATTCAACCAAACCATCCGCCTCAAACAGCAAGGGTACAATCGCACCGAGATACAGCGGGAAGTTGACCGCGACGACTTCTTGCGGAACAACAAGTGCG
CGGTCGTCGGGAAAGCCCTCCAAACGTGGAACTCCTACCAGTCACTCAAAGACTGGTGGGAGAACCAAGACGACCCTGACGGCGGGAAGCCGACCCCGCC
GAGTACCGACAAATCTGGTGTGTACCCGCTCGTGATGGCGCACGCGGAAGGCTACCGCCTCACCGTAGATGACGACACGAACCGCGTCCGGTTCCGCATC
AGCCCGAAACCCTACAAGAAGGTGAAGGGCCACCTTCGCGGAGAGCCGGACGCGATGGATGAACTTCGAGACGCCATCACGTCGGATAAGATCGATGTGG
GGCAGGCAGAACTCCTGTACCGCGATGGCGTGTACTACCTACACGTCACGGTCACACGCGAGTTCGACGTGCCCGAACCAGACACCAGCGACACGCTCGT
CGGCGTGGACATCAACGAGCGTAACGTCGCACTCACCGCCCTCGACCGCGAGACGATGCGGACAAAGGGCACGCTCGTCCTCGACTATGGACGGGTGAAG
GAGGAACGTCAACGCTACCATACAATCACCACTCGCTGTCAGGAACACGGCAAGACGAGCATCCACCGGAAACTCGGTGACGACGAAGAGCGATTCACCG
AGTGGGTGTTGCACCGTCTCTCCCGTGCGGTCGTGGAGTTCTCGGAGCAGTTCTCGAATCCGGTTATCGTGTTCGAGGATATGACCGGCATCCGCGACGA
AATCAAGTACGGGACGTATATGAACCGGCGGTTGCACAAATTGCCGTTCCACAAGTTCGAGACGTTCGTCTCGTATAAGGCGACGTGGCGAGAGATTCCT
ATGGATACGGTGGATGCGTACTACAATTCGAAGACGTGTTCGTGCTGTGGTGAGCGTGGGAGTCGTCAAGGACGGCGTTTCCGGTGTACGAACGACGAGT
GTGTTGTGGTGCAAGATCACGCCGACCGGAATGCGTCGGTGAACATCGCGTGGCGCGAGACGCTGAAACTCGACGGTAACGAATCGAATTACCGGACTCA
CAAAACCCAACCACAAGTTCGGTTGGTGCGTCTGTCCGGGTCGGGGCGCGTAAGCCGCCCAACCTCATCCCGTTCCCTCGCGGAACAGGGAGTGTTAGCG
CACGGCTGAGGGAACTACAAAAGCCTCGGGCCATCGTGCCCGAGGCTGTTTAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa46062-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEEYRSHAHSVSSCKYHFVWCPKYRHPVLNVVEDDVRELFSETADHFGHEILALEIADDHVHLFVQCDPKHSPADIARQFKSYSGKHLLERYPEIRESYF
WGGGFWKVGYYVGTTGEVSEEVVERYIEQTEH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1305 bp434 aa5051809+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MGEEATKTIQTRLQIASGERSWLHDARLASREIFNQTIRLKQQGYNRTEIQREVDRDDFLRNNKCAVVGKALQTWNSYQSLKDWWENQDDPDGGKPTPPS
TDKSGVYPLVMAHAEGYRLTVDDDTNRVRFRISPKPYKKVKGHLRGEPDAMDELRDAITSDKIDVGQAELLYRDGVYYLHVTVTREFDVPEPDTSDTLVG
VDINERNVALTALDRETMRTKGTLVLDYGRVKEERQRYHTITTRCQEHGKTSIHRKLGDDEERFTEWVLHRLSRAVVEFSEQFSNPVIVFEDMTGIRDEI
KYGTYMNRRLHKLPFHKFETFVSYKATWREIPMDTVDAYYNSKTCSCCGERGSRQGRRFRCTNDECVVVQDHADRNASVNIAWRETLKLDGNESNYRTHK
TQPQVRLVRLSGSGRVSRPTSSRSLAEQGVLAHG

 

Blast result :
Comments
ISH34 is 94% (TnpA : the transposase) and 96% (TnpB) aa similar to ISNph6.
References
1] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91: 335-346