ISfinder Mirror Site

ISSen6

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006511	ND	Salmonella enterica	Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150

DNA section

IS Length : 1818 bp

Ends

Left end : CTTAACTCCAAAGGGTACTCCCGCCGACATAAGGCGGGAGGGGAATTTGGACGGGCTTGAAAAGCCCGAAGGATCATCAGCCACGCTGATGTTGAATGTA II struct. : Yes
Right end : CCGTCTGAAACAGGAACCAGACGCTGAGCAATCAGTCGTCACCCACTAATGGGAATTCCCCACCTGAGTCACGAAGTGCGAAGGTGGGGGAGGATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ACGCTGTGCA	CCAT	TGTTGTTGTC	TCAA

DNA sequence

CTTAACTCCAAAGGGTACTCCCGCCGACATAAGGCGGGAGGGGAATTTGGACGGGCTTGAAAAGCCCGAAGGATCATCAGCCACGCTGATGTTGAATGTA
TTGCTTCACAACTTCCAGCGGCGCACCTCCGCACGAGCCAGCAAAGTATGACCTCGACCAGAGCACGGCTTTTCCGTATGCTCCCCGCAAATCAATAAAT
TCGTTTCGCAGACGGCGGGATGTCACAGCTTTCAGTGAGTTAACCAGTACCGAAAGCTGCACTGTTGGTGGGTATTCGATCAGCATGTGGACATGATCGA
CGTCTCCGTTACTTTCTTTAAGTTCTCCGCCAAAATCACGGCAGACTTCTGCTGCATACTGATGGAATGCAGCATAGTGTGACTCACCCAGTATCTTTCG
ACGGTATTTTGTTACAAAGACAAGATGAACATGCAAAAGGAAGGCGGCATGTCTTGAACGATTAATTTTATGTTTTTGTATTGAAGTTGACCGTAAAAAC
AGTAACGTAGCAAACAAATCTCCTACACACTGAGTGATGATGCTAATCCTGAAAGCCTACAAATTCAGACTGGAACCAACGCCTGAACAGTCGCAGCGTT
TGCGGCAGTTATGTGGTTGCGCCCGTTTTGTCTGGAATTTAGGTCTTGCGGAGACAAAGCGCATCCTTGGTTCAGGCGAAAAGTTACCTTCGGCTTTCGA
GTTGAACAGGATGCTTACAGTGTGGAAAAAAATGCCGGGACACATCTTTTTACAGGATGCTTATACCGACAATCTGCAACAAAAGTTGAAAGACCTGCAT
ACTGCATGGAAACGTTGCTTTGATAAAAAGCTCGCAGCTAAGGCCCCGGTATGGAAACGAAAAAATGAGGGCAGAGACTCAATCCGTTTTGTGAACTTTG
AGAAATATTGCCGCCTTGAAAATCGCAGAGTGAAGCTACCGTCAGGTCTTGGGTGGGTAAAATTCCGGCAATCTCAACGTGTGAACGGTAAAATCAAAAA
TGCGACAATCAGTCAGTTAGCGGGACAGTGGTATATCTCGTTTCAGGTTGAAGTTGAAACGGCAGAACCCAATCACACAAGCACAACGATAGTCGGTCTG
GACGCAGGAGTAACAAAACTCGCCACGCTTTCAGACGGTACGGTATACCAACCAGTCAACAGTTTTAAAGCAAGTCAGCGCAAGCTGGCAACACTCCAGC
GGCAGTTAAGCCGTAAAGTCAGATTCAGCAGTAACTGGCAGAAACAAAAGCGAAAAGTCCAGCATCTGCACTCGCACATAGCCAATATCCGGCGCGACTA
CCTTCATAAAGTTACCAGTGAAATCAGCAAAAACCACGCGATGATAGTCATTGAGGATTTGAAGGTTAGTAACATGTCGAAATCGGCAAAAGGTACAGCA
GAACAGCACGGACGAAACGTCAAGGCCAAATCAGGTCTGAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGAGTACAAACAGC
TCTGGCGTGGCGGTCAGGTGCTTGCGGTTCCGCCAGCATATACAAGCCAGCGTTGCGCGTGCTGTGGTCATACCGCGAAAGAAAATCGCCTGTCACAAAG
TAAATTTGTGTGTCAGGCATGCGGATATACAGCGAACGCTGATGTAAACGGCGCTCGTAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGGG
ATGGTGCAGTCAGGCCGCCCGTCTGAAACAGGAACCAGACGCTGAGCAATCAGTCGTCACCCACTAATGGGAATTCCCCACCTGAGTCACGAAGTGCGAA
GGTGGGGGAGGATGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
441 bp	146 aa	517	77	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MFATLLFLRSTSIQKHKINRSRHAAFLLHVHLVFVTKYRRKILGESHYAAFHQYAAEVCRDFGGELKESNGDVDHVHMLIEYPPTVQLSVLVNSLKAVTS
RRLRNEFIDLRGAYGKAVLWSRSYFAGSCGGAPLEVVKQYIQHQRG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1209 bp	402 aa	537	1745	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MMLILKAYKFRLEPTPEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMLTVWKKMPGHIFLQDAYTDNLQQKLKDLHTAWKRCFDKKLA
AKAPVWKRKNEGRDSIRFVNFEKYCRLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQLAGQWYISFQVEVETAEPNHTSTTIVGLDAGVTKLATLSD
GTVYQPVNSFKASQRKLATLQRQLSRKVRFSSNWQKQKRKVQHLHSHIANIRRDYLHKVTSEISKNHAMIVIEDLKVSNMSKSAKGTAEQHGRNVKAKSG
LNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFVCQACGYTANADVNGARNILAAGHAVLACGGMVQSGRPSETGT
RR

Blast result :

Comments

ISSen6 is 86% (TnpA : the transposase) and 82% (TnpB) aa similar to ISEc41.

References

1] ISfinder submission (2013)
2] McClelland,M., Sanderson,K.E., Clifton,S.W., Latreille,P., Porwollik,S., Sabo,A., Meyer,R., Bieri,T., Ozersky,P., McLellan,M., Harkins,C.R., Wang,C., Nguyen,C., Berghoff,A., Elliott,G., Kohlberg,S., Strong,C., Du,F., Carter,J., Kremizki,C., Layman,D., Leonard,S., Sun,H., Fulton,L., Nash,W., Miner,T., Minx,P., Delehaunty,K., Fronick,C., Magrini,V., Nhan,M., Warren,W., Florea,L., Spieth,J. and Wilson,R.K. (2004) Nat. Genet. 36 (12), 1268-1274.