ISfinder Mirror Site

ISNpe20

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003373	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624 plasmid pNATPE01

DNA section

IS Length : 1529 bp

Ends

Left end : AGAATACAAGGAGAATACCTGCGCCTTGAGGCGCGGGATGAATCCGACAACTCTGACACACCCTACTATCGATAGCACGGTTGGATATTCCACTGTTCCC II struct. : No
Right end : CCAACCGCAGGATCAAGTGGTAGACTGCAAACCCTAATATCCCAATCCAGCGGCGCGGTGTCGTGGGATTCCCGCGTCTTCAGGCGCGGGAGGATGTCAA II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GTCTGTCGGTCG	TTAT	GGGATTAGTGTC	tcaa

DNA sequence

AGAATACAAGGAGAATACCTGCGCCTTGAGGCGCGGGATGAATCCGACAACTCTGACACACCCTACTATCGATAGCACGGTTGGATATTCCACTGTTCCC
AACCCGAACTTTTACTTACGAATCAACTATAACCTGTTATAGAGCGTTCCAAATGCTGGAAGTCCACCGAACCCACCAAGCGAAAATCCTCAACCACGGT
CAGGTAGAGGAGTCGCTTGACAGGCACGGCTGGTCAGCCAGCAAGCTCTGGAACGTCGCTAACTACTACTCCCGCGAAGTATGGGATGAAACGGGTGAAA
TCCCCGATCACGGAGACCTCAAAGACGAGTTGAAGACCCACAACAAGTACAAAGGACTACACAGTCAGTCCAGTCAGCGCGTTCTGGAGGAACTCGCTGA
ATCCTTCAACTCGTGGTACGGCTCCGAAGACGACAGGGACAACCCACCCGGCTACCGCAAAGAAAACTACTACGACCAACAGGGTCGTCGCGTCCACGAA
GAACACCCGCGTAGCACGGTGACGTGGAAACAGAAAGGCATCCGTCACGACTCAAAGAACGATCGGGTTCGCCTCTCAAAAGGTCGAAATCACAAAGAGT
ACCCCCGAGCGAGAGAGTACATCCTCGTTGAGTACGAAACCGCACCCGGCGTCACCGTCGAAAATCTACAACAGGTTCGCGCCGTCTACGACAACTCAAA
CGGGCACTGGGAACTACACCTCGTCTGTAAAGACGAAGTTGAGACTCCTGACGCTCCCGGCACCGAGACGGCAGGTATCGACCTCGGTATCTGTAACTTC
GCTACCGTTGCGTACAGTACCGAGGAAGCCGACCTCTACCCCGGCAACCGGCTGAAACAGGACGGCTACTACTTCCCAAAAGAGATCGCCAAGTGCGACG
ATTCGGGTGGCGAAGAAGCCACCCGTCTACACGCGAAATGGTCGGAGCGGCGCACTCACTTCTTCCACAGTCTCGCCAAACATATCGTTCAGCGGTGTGT
TGAAAAACAAGTTGGTCGTATCAACGTGGGGGACTTGGAGGGTATCCGAGAGGACGAAGATGGTGGCTCAAAGAACTGGGGTCGGCACGGAAACCTCGAC
TTGCACGGGTGGGCGTTCGACCGCTTCACGGACCTTCTCGAATACAAGGCGAAGGTCGAGGGTATCGAGGTTGTGGAAGTATCTGAGCGTGACACGAGCA
AGACGTGTTGTATGTGTGGGAGAGAAAACGAGAGTCAGCGTGTTGAACGTGGTCTGTACGTCTGCGAGGAGTGTGATGCGGCGTTCAACGCTGACGTGAA
TGGGGCGGAGAACATCCGTCTCGACATGAACGAAAGTAACTCCGAGTCTTCGGCCAGTTTGGACGGGGATAGGAGTACCGGCTGGTTGGCACAGCCCGGA
GTCTACCTGTATGACTTGTCCAGCGGATTCCAACCGCAGGATCAAGTGGTAGACTGCAAACCCTAATATCCCAATCCAGCGGCGCGGTGTCGTGGGATTC
CCGCGTCTTCAGGCGCGGGAGGATGTCAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1314 bp	437 aa	153	1466	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLEVHRTHQAKILNHGQVEESLDRHGWSASKLWNVANYYSREVWDETGEIPDHGDLKDELKTHNKYKGLHSQSSQRVLEELAESFNSWYGSEDDRDNPPG
YRKENYYDQQGRRVHEEHPRSTVTWKQKGIRHDSKNDRVRLSKGRNHKEYPRAREYILVEYETAPGVTVENLQQVRAVYDNSNGHWELHLVCKDEVETPD
APGTETAGIDLGICNFATVAYSTEEADLYPGNRLKQDGYYFPKEIAKCDDSGGEEATRLHAKWSERRTHFFHSLAKHIVQRCVEKQVGRINVGDLEGIRE
DEDGGSKNWGRHGNLDLHGWAFDRFTDLLEYKAKVEGIEVVEVSERDTSKTCCMCGRENESQRVERGLYVCEECDAAFNADVNGAENIRLDMNESNSESS
ASLDGDRSTGWLAQPGVYLYDLSSGFQPQDQVVDCKP

Blast result :

Comments

ISNpe20 is 89% aa similar to ISHli3.

References

1] Pfeiffer, F. (2013) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank.