ISfinder Mirror Site

ISNpe15

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003373	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624 plasmid pNATPE01

DNA section

IS Length : 1069 bp

Ends

IR Length : 19/23

IRL : CAGCGTGTCATAGAAAGCGTCTCGTGCAAGGCAGCAGGGTGCGGAAAGTG
IRR : GAGCGTGTCATAGAATCCATCTCATAGCTTGAGCTTCTCACATCCCGGAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTGGGTTGAT	ACTGCTA	GACAGAACTA	7

DNA sequence

CAGCGTGTCATAGAAAGCGTCTCGTGCAAGGCAGCAGGGTGCGGAAAGTGTGTCCAGCACAATCGCAGCCCTGCAAGACGTAACTTCGGTAGACGAGTTC
TTGAATGCGGCGGCTACCGAGACAGTGCCGCTATTCGAGTACCTTGAGTTCGAGTTTCTGCTGGAGTACGATGTGTTCGCCCCCGCTTCGAGGGGGCGAA
CACGAGTTCATCAGCCACCAGATCTCTTTCGTGGTTTTCTGCACTGCTATTACAAGAACATCTACGGTACACGCCCCGTCACGCGTGAACTCCAGCATAC
ACTCGTCTGGTACTACTGCGGACTTGACAAACCTCCATCTAGAGACACGGTTGACCGCTTTCTCACTGACCTCGAACATGTCGTCGACGATGTCTTCGAC
AGGCTCGTCGAGCAGGCCGCCGTCCGCGGCCTGCTCGACTCAACATATTCCATTGATTCGACGCACATTGAGGCGATTCGGTACAACGACGCAGCGTCAT
GGAACTACGATCCAACAGCTGAAGAGTACTACTACGGCTTCGGCTGTACGATCGTCTCAAGCGGAGCAAAGATCCCGATAGCGGCGGAGTTTACACAGGC
TAAACAGGCATCTCAAGAGACGGCGATGCGCGTCACGAGTGACGCGCTCGCCGTCGATACACCGACCTGGATGCTTGGAGACAGTGCCTACGACATCCTC
GACTGGCACGACCACCTGCTGGCCGCAGGGGTCGTGCCAGTCGCCCCGTACAACCCGCGAAACACCGACGATCCGAAAGATATCGAGTACAGGATTGAGG
ACCGCATCGAGGAACACAGCGAGGACGTCCAGCTCAAACAATCCGTCTTAGACGAGACGTACAACCGCCGGACAGGGGTCGAACGAACCAACGACGCGGT
CAAGGACTGCGGCCTCGGGCACGTTCGCGCCCGAGGCCGTGTTCACGCACGGACAGAAGTGTTCGTCGCGCTCTGCCTACGACTCGTCGTTGCCATCACC
AACTATGAGCGAGGAGACGATCCGGGATGTGAGAAGCTCAAGCTATGAGATGGATTCTATGACACGCTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
999 bp	332 aa	50	1048	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSSTIAALQDVTSVDEFLNAAATETVPLFEYLEFEFLLEYDVFAPASRGRTRVHQPPDLFRGFLHCYYKNIYGTRPVTRELQHTLVWYYCGLDKPPSRDT
VDRFLTDLEHVVDDVFDRLVEQAAVRGLLDSTYSIDSTHIEAIRYNDAASWNYDPTAEEYYYGFGCTIVSSGAKIPIAAEFTQAKQASQETAMRVTSDAL
AVDTPTWMLGDSAYDILDWHDHLLAAGVVPVAPYNPRNTDDPKDIEYRIEDRIEEHSEDVQLKQSVLDETYNRRTGVERTNDAVKDCGLGHVRARGRVHA
RTEVFVALCLRLVVAITNYERGDDPGCEKLKL

Blast result :

Comments

ISNpe15 is 95% aa similar to ISNpe2.

References

1] F. Pfeiffer (2013) Direct submission.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank.