ISHvo10

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001955 ND Haloferax volcanii
Haloferax volcanii DS2 plasmid pHV4
Haloferax volcanii DS2
DNA section
IS Length : 1462 bp

Ends


IR Length : 14

IRL : gtgctgacttgATGGAAATTCTGCTATCCCTGCGGAGCGTGAATCAAGCA
IRR : --tattcacctATGGAAATTCTGCTGGCTCAACTAACCACCGCTAGCGAT

Insertion site


Left flankDirect repeatRight flankDR Length
GACAAGAACCTCTAGAGGCTACAT0
GTTGCTGCGGCCCAGAGCGCAAGT0

DNA sequence

GTGCTGACTTGATGGAAATTCTGCTATCCCTGCGGAGCGTGAATCAAGCATGGGACGCAAGAGACCGAGCTGTATTCCGTGCCCCAGAGCACGTGGTGCT
GACTGGTCCGAGTGCGAAAGAAGCCGAGTTATGGGCGTCACAGCCCGGATGCCGCTCCTGGCGGCATCCGGGAAAGCCCGCATAGGTGAAGTTCTGATTC
ACGCACCGCCGTCCTATCCCGGAGGAACAAGCAATGTACCTCGGAATCGACTTACACAAACGGTACGCACAGGTAGCAGTAATCGACAGCGAAGGCCAGA
TTGTCGAAGAGGTTCGCGTCAAAAACGCGAACCTCGACGATCTCGCTCGACGCTACGCTGGCTCTCGTGCGGTCATCGAGGCGACCAGCAATTACTACCA
CGTCTACGATACGCTCGCGGAGTACTTGGACGTAACCGTCGCTCATCCAGGTAAACTGACGCTCATCGCTCACTCCGACAAGAAAACCGACCGCGTCGAC
GCCAAAGAACTCGCGCGGTTGCTTCGACTCAACTCCGTTCCGCAGAGTTACGTTCCCACCGACGAGATCAGGCAAGCCCGCGCACTCGTGCGCGGGCGAC
AGACATTGGTCGAAGATCGCACCAAGTTCGCCAACAAGATCCATGGCTTGCTCGCTGACAACGGTATCACCCGCGAGGTGAAGCCACTGAGCGTGAAGGG
ACGAGAGTTCCTCCGGGAACTCTCGCTCCCGTCCCCGTGGGACGCGTTGTTGGAGTCGTACCTTGACGTGGTTGAGACCCTGACCGAGCAGATGACACAG
CTGGAAGCTGCGATTGAGGAGCGCGCTGGGTCTCTGAAGGAGACCCAGCTGCTCATGACGATTCCTGGAGTCAGTTACTACTCCGCGTTGCTGATCTACG
CAGAACTTGGAGAAGTTGATCGGTTCGACAGCCACAAGGAGGTCGTGAGTTACATGGGGCTGAACCCGACGATCCGCGAGTCCGGCGACTCGCGGATCGA
GGGTGGTATCTCGAAACGTGGTTCAGGACGGGTGCGGTGGATTTTGGTTCAGAGTGCATACTCAGCAGTGTACACGTGTAAAGACGAGTATCTGAGCCGG
TTCTTCCACCGGTTGAACCGTCGGATGAACCCGAAAAAAGCAATCGTTGCGACGGCGCGAAAACTGCTCGTCTCGATGTACTACATGCTCGTCCGGGAGG
AAGTCTACGATCCACCGGGGGTGAGCGCCTGAGGAAGGGAATCTGAGGAACGCCAGTCCGGCGTTCCTCAGTGGCCGGATGGAACCAGCGTGAGCGACAG
CTATCGCGGAACTCGTACCGACCGCCTATCGGTTGTGGAGGTCTCAATCACCACGTTGCCCAGTGGTTTTTCACCGTTGAAGAACGAGAGACACGGAATC
GTGCGAACAACTTTATCGCTAGCGGTGGTTAGTTGAGCCAGCAGAATTTCCATAGGTGAATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
999 bp332 aa2341232+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MYLGIDLHKRYAQVAVIDSEGQIVEEVRVKNANLDDLARRYAGSRAVIEATSNYYHVYDTLAEYLDVTVAHPGKLTLIAHSDKKTDRVDAKELARLLRLN
SVPQSYVPTDEIRQARALVRGRQTLVEDRTKFANKIHGLLADNGITREVKPLSVKGREFLRELSLPSPWDALLESYLDVVETLTEQMTQLEAAIEERAGS
LKETQLLMTIPGVSYYSALLIYAELGEVDRFDSHKEVVSYMGLNPTIRESGDSRIEGGISKRGSGRVRWILVQSAYSAVYTCKDEYLSRFFHRLNRRMNP
KKAIVATARKLLVSMYYMLVREEVYDPPGVSA

 

Blast result :
Comments
ISHvo10 is 97% aa similar to ISHvo9.
The IR of this IS are not at its termini. In the IS sequence as given 11 nt separate IRl from the left-hand end of the element and 9 nt separate IRr from the right-hand end
References
1] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S.,Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605