ISH32
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AM774418 | ND | Halobacterium salinarum | Halobacterium salinarum R1 Natrinema pellirubrum DSM 15624 Halobacterium salinarum DSM 671 |
DNA section
IS Length : 1625 bp
Ends
IR Length : 16/17
IRL : CATCCGTTCTAGTTTAGCAGAGTGTACCGTCGTCTGATTAGATATCGTTG
IRR : CATCTGTTCTAGTTTAGTGACGGAGTGAAGCAGGATCTCAGCGGAGACTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTCGATTCA | CCCGCCCTCC | AGAGGATGCC | 10 |
| GGCGGCGCGGCT | GTCTACTCAG | GGGAATCTTCTG | 10 |
| ACGGACGGATCT | TCGAAGGTAC | AGTCAACCGGTC | 10 |
| AGCCGATATCTT | CCCAAGTCAT | AGATCAAATTTC | 10 |
| CGGGTTCTCCTT | GCAGACGACG | AGGTCGTCGAAC | 10 |
| GGCGACGACGCT | GTAGTAGGAC | AGAATAATGAAC | 10 |
| AGCGAAAATCCG | GTGTTTGATC | AGTGGGCGAATG | 10 |
| TCGACGATCCCT | GCTTGCCGGT | AGGCTTCGTCGA | 10 |
| ACCCCACGATGT | CCGCAGTTAC | AGCGAGCACCTC | 10 |
| TGGGGTGTCGCC | CCTTTTGAAC | AGAATCTCCACG | 10 |
| TTCACCGTTCGT | GATGGGCTCG | AGAACGCTAGAG | 10 |
DNA sequence
CATCCGTTCTAGTTTAGCAGAGTGTACCGTCGTCTGATTAGATATCGTTGTAACTACCAGTCAGAGAAATCCGGTGGATAGTGTGACTTACTCCCCACCG
GATTCAGTGATTGTTGATCGGATTCAAAGAGCGTTTCCCTCCGATGAGTTGCGCGAGCGCGCTCGCGCAACGAATCTCGTCGAGCGTGAACGGAAATTCG
ACGCTGTTGCGCTGTTCTACACGCTTTCACTTGGCTTCGCTGCTGGGTCAGACCGATCTGTACAGGCTTTTCTCGAACGATTCGTCGAAATGTCCGACTG
CGATGAACTCTCCTATGCAACCTTCCACGGGTGGTTCTCTCCACCGTTCGTTGCACTCCTTCGAGAGATTCTCGATGATGCCATCGAGAATCTCGATACC
AGAAATGCCGACCTTAGCGGACGTCTCGAACGCTTTCGAGACGTCCTCATTGCCGATGGGAGCATTGTTTCTCTCTATCAAGACGCCGCGGATGTGTACG
CTGCGACCGGTGACGATCAGGCCGGTCTGAAACTTCACCTAACAGAATCACTCTCAACTGGCCTTCCAACACGGTACCGAACAACTGACGCTAAAACCCA
AGAACGGAGCCAGCTACCCACCGGCGAGTGGGTAGCTGGCGCACTTGTCTTGCTCGATCTCGGTTTCTACGACTTCTGGTTGTTCGACCGCATCGACCAG
AATAACGGCTGGTTCGTCTCCCGTGTGAAAGACGACGCAAACTTCGAGATCGTCGAAGAGCTCCGGACGTGGCGGGGGAACAGTATCCCGCTCGAAGGAG
AGTCGCTGCAGGACGTCCTTGACGACCTGCAGCGACAGGAAATCGATGTTCGCATCACCCTCTCGTTCGAGCGAAAGCGAGGGTCGTGCACCAGCACGAC
CCGAACGTTCCGACTGGTCGGTGTTTGGAACGAGGATACTGAAGAGTACCATCTCTATTTGACAAATCTCTCGAAAGACGACTATAGTGCGCCCGATATC
GCACAGCTCTATCGGGCGCGCTGGGAAATAGAATTATTGTTCAAAGAACTGAAATCACGCTTCGGACTTGACGAAATCAACACGACCGATCCGTACATCA
TCGAAGCTCTGGTCATCATGGCCGCAATCTCACTGCTGATGAGCCGTGTTATCGTCGATGAACTCCGGAAACTGGACGTGAAACAACGGGAAAGCGCCGA
CGACGCCACAGCGTCGTCGCCGCAACTTCCTCGTCGACGATGTTCTCACGCTGTCGAACGCCATTCACACCTGATTCAGTTGTACGTGATGCTCGATTTA
GGGTACGAACTTCCGGATTTAGATGAGTTGTTGCTGTGGGCTTCACGTGATCCAAATCCGCATCGACCGCGGTTACGTGAGCAGGTTGAGTCAGGCGAGT
TCTGGTAACGAACTCGCCTGACGACCGGGGAAGCACCTGTGTCGATAGCGGCAGCGCTGGAACGGCGACGCACTCACGGCTGTGAGTGCGCCGCCTCGAA
GGTATCACACAACAAATCAGCGACTGCCTGCTCCAGCGAACCCAGTCTCCTGAGCTAATCCGCCTCAATTCGGTATAGTCTCCGCTGAGATCCTGCTTCA
CTCCGTCACTAAACTAGAACAGATG
GATTCAGTGATTGTTGATCGGATTCAAAGAGCGTTTCCCTCCGATGAGTTGCGCGAGCGCGCTCGCGCAACGAATCTCGTCGAGCGTGAACGGAAATTCG
ACGCTGTTGCGCTGTTCTACACGCTTTCACTTGGCTTCGCTGCTGGGTCAGACCGATCTGTACAGGCTTTTCTCGAACGATTCGTCGAAATGTCCGACTG
CGATGAACTCTCCTATGCAACCTTCCACGGGTGGTTCTCTCCACCGTTCGTTGCACTCCTTCGAGAGATTCTCGATGATGCCATCGAGAATCTCGATACC
AGAAATGCCGACCTTAGCGGACGTCTCGAACGCTTTCGAGACGTCCTCATTGCCGATGGGAGCATTGTTTCTCTCTATCAAGACGCCGCGGATGTGTACG
CTGCGACCGGTGACGATCAGGCCGGTCTGAAACTTCACCTAACAGAATCACTCTCAACTGGCCTTCCAACACGGTACCGAACAACTGACGCTAAAACCCA
AGAACGGAGCCAGCTACCCACCGGCGAGTGGGTAGCTGGCGCACTTGTCTTGCTCGATCTCGGTTTCTACGACTTCTGGTTGTTCGACCGCATCGACCAG
AATAACGGCTGGTTCGTCTCCCGTGTGAAAGACGACGCAAACTTCGAGATCGTCGAAGAGCTCCGGACGTGGCGGGGGAACAGTATCCCGCTCGAAGGAG
AGTCGCTGCAGGACGTCCTTGACGACCTGCAGCGACAGGAAATCGATGTTCGCATCACCCTCTCGTTCGAGCGAAAGCGAGGGTCGTGCACCAGCACGAC
CCGAACGTTCCGACTGGTCGGTGTTTGGAACGAGGATACTGAAGAGTACCATCTCTATTTGACAAATCTCTCGAAAGACGACTATAGTGCGCCCGATATC
GCACAGCTCTATCGGGCGCGCTGGGAAATAGAATTATTGTTCAAAGAACTGAAATCACGCTTCGGACTTGACGAAATCAACACGACCGATCCGTACATCA
TCGAAGCTCTGGTCATCATGGCCGCAATCTCACTGCTGATGAGCCGTGTTATCGTCGATGAACTCCGGAAACTGGACGTGAAACAACGGGAAAGCGCCGA
CGACGCCACAGCGTCGTCGCCGCAACTTCCTCGTCGACGATGTTCTCACGCTGTCGAACGCCATTCACACCTGATTCAGTTGTACGTGATGCTCGATTTA
GGGTACGAACTTCCGGATTTAGATGAGTTGTTGCTGTGGGCTTCACGTGATCCAAATCCGCATCGACCGCGGTTACGTGAGCAGGTTGAGTCAGGCGAGT
TCTGGTAACGAACTCGCCTGACGACCGGGGAAGCACCTGTGTCGATAGCGGCAGCGCTGGAACGGCGACGCACTCACGGCTGTGAGTGCGCCGCCTCGAA
GGTATCACACAACAAATCAGCGACTGCCTGCTCCAGCGAACCCAGTCTCCTGAGCTAATCCGCCTCAATTCGGTATAGTCTCCGCTGAGATCCTGCTTCA
CTCCGTCACTAAACTAGAACAGATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1332 bp | 444 aa | 74 | 1405 | + | No |
Chemistry : DDE
ORF sequence :
MDSVTYSPPDSVIVDRIQRAFPSDELRERARATNLVERERKFDAVALFYTLSLGFAAGSDRSVQAFLERFVEMSDCDELSYATFHGWFSPPFVALLREIL
DDAIENLDTRNADLSGRLERFRDVLIADGSIVSLYQDAADVYAATGDDQAGLKLHLTESLSTGLPTRYRTTDAKTQERSQLPTGEWVAGALVLLDLGFYD
FWLFDRIDQNNGWFVSRVKDDANFEIVEELRTWRGNSIPLEGESLQDVLDDLQRQEIDVRITLSFERKRGSCTSTTRTFRLVGVWNEDTEEYHLYLTNLS
KDDYSAPDIAQLYRARWEIELLFKELKSRFGLDEINTTDPYIIEALVIMAAISLLMSRVIVDELRKLDVKQRESADDATASSPQLPRRRCSHAVERHSHL
IQLYVMLDLGYELPDLDELLLWASRDPNPHRPRLREQVESGEFW
DDAIENLDTRNADLSGRLERFRDVLIADGSIVSLYQDAADVYAATGDDQAGLKLHLTESLSTGLPTRYRTTDAKTQERSQLPTGEWVAGALVLLDLGFYD
FWLFDRIDQNNGWFVSRVKDDANFEIVEELRTWRGNSIPLEGESLQDVLDDLQRQEIDVRITLSFERKRGSCTSTTRTFRLVGVWNEDTEEYHLYLTNLS
KDDYSAPDIAQLYRARWEIELLFKELKSRFGLDEINTTDPYIIEALVIMAAISLLMSRVIVDELRKLDVKQRESADDATASSPQLPRRRCSHAVERHSHL
IQLYVMLDLGYELPDLDELLLWASRDPNPHRPRLREQVESGEFW
Blast result :
Comments
ISH32 is 94% aa similar to ISH30.
In plasmid pHS3, this element has been targetted by an ISH8 element after base 1501, causing an 11 bp target duplication (CCGCCTCGAAG). A subsequent inversion between this and another copy of ISH8 has separated the parts of this element. Reconstruction of the original insertion site identifies a 10 bp target duplication.
File updated : May 7 2015 : complete copies are found in Natrinema pellirubrum DSM 15624 (NC_019962)
In plasmid pHS3, this element has been targetted by an ISH8 element after base 1501, causing an 11 bp target duplication (CCGCCTCGAAG). A subsequent inversion between this and another copy of ISH8 has separated the parts of this element. Reconstruction of the original insertion site identifies a 10 bp target duplication.
File updated : May 7 2015 : complete copies are found in Natrinema pellirubrum DSM 15624 (NC_019962)
References
1] Pfeiffer F, Schuster S.C., Broicher A, Falb M, Palm P, Rodewald K, Ruepp A, Soppa J, Tittor J, Oesterhelt D. (2008) Genomics 91: 335-346
2] Pfeiffer F. (2013) Direct submission to ISfinder
2] Pfeiffer F. (2013) Direct submission to ISfinder