ISfinder Mirror Site

ISSiac5

Family ISLre2
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_019892	ND	Singulisphaera acidiphila	Singulisphaera acidiphila DSM 18658

DNA section

IS Length : 1429 bp

Ends

IR Length : 22

IRL : GGGTGTAACTCTGGAATCTGGAAATTCTGCCGCCTCCCTGGTATGACGGG
IRR : GGGTGTAACTCTGGAATCTGGAGATCAAGCGGCGGTCCGAGTCCAGAGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCTCACTGG		GCTCACTGTT	0
GATCTGCAAA		GCGGCAATCG	0

DNA sequence

GGGTGTAACTCTGGAATCTGGAAATTCTGCCGCCTCCCTGGTATGACGGGATGCTCCCCGACACCCCTGGAGGCGAAGCGATGGAGCGGACGGTCACGGT
CAGGACGGAGTTGGAGTCGATCTTGGTCGAACAGGCCCTGGCGATGGCGCGCGAGTTGGAGGCCGTCACCGATGCGGCTCCGGACGGTCAAGTCCTGGCC
GTCGGCGAGTTGACCGCTGTTCGACTCGGCCGCGAGTTGACCCGTGTCGCCCTGGAGTCGGCCTTGCAGCAGCAGGCGCAGGCGGCCGAGAAAAAGGGGC
TCCCGGCCGAGCCTGCCCCTGCGGCGGCCGTCGCGCGATCAAGGACCAGACGGCCAAGACGACCGTGATCGCCGCCGGCCGGGTGACGCTGCGTCGTCGC
TCGCTGCGATGCCACGGCTGTGGCCTGACGGCTCATCCCCTCGACGCCCGCCTCGGCGTCGACGACTTCCTCAGCCCGCACGCCACCCGGTTGGCCTGCC
TGGCGGCGGCGAGTTGGTCATTCGACGTCGCCTCCGACCGCCTGCACGAGTTCGCTGGCGTCCCCATCAACGGCGAGACGATCCGCCGGCATAGTCATCG
CGCCGCCCAAGCTCTGACGCGGCGGCGCGAGCAGGAGCCGCCGAAGGCGCACTTCGAGGACGCCGAGGGGGAAATTGAGTTCCTGACTGACGGCGTCATG
GCTCCGACCCGCGGCGGCTGGCGGGAACTGAAGATGGCGTTGTACCTGAAGCGACCGCTCGGCGAACCGGCCGAACCTGACGCCTGGGCCGAGCGGGAAC
TGCCGCGGCCGACGGCGCAGGCGGCCTATGCCACGGTCGCCGATAGCGAGACATTCGCGGCGCGATGGACGCCGCTAGCCCAGGCGTTGGGGATCGAGCC
GGCCGGTTCGTTGACGGTCCTGGGCGACGGCGCCGAGTGGATCTGGAACGCGGCCTCGACGCAGTTCCCCGCGGTCACGCAACTCCTGGACGTCTTCCAC
GCCTGCGAGCATCTGGCCTCGGCGGGCCGCGCCCTGTTCGGCGACGAGGCCGCCGACTGGGTCGAACGAGGGCGGCACTCGCTCCTGGCCGACGGCTGGC
CGGGGCTGCTCGACCACGTCGGAGCCACTCCGGTCGCGGGCCGGACGACGGCCGGTCAGGCCGGCCTCGATGAACTGATCGGCGACTTCGCCAAGCATAC
CGACCGTCTCGGCTATTACGGTCGACTCCGCACGGGCCGCTCGATCGGTAGCGGCGGAGTGGAGAGCCTGGCGCGGCGGATGGGCCGTCGGCTCAAAGTC
CCGGGACGAGGCTGGTGCGTGGATCACCTGGAGGGCATGGCGTCCCTGATCGCCACTGCCGATACCCCGGAATGGGAGAGCCTCTGGACTCGGACCGCCG
CTTGATCTCCAGATTCCAGAGTTACACCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
288 bp	95 aa	81	368	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MERTVTVRTELESILVEQALAMARELEAVTDAAPDGQVLAVGELTAVRLGRELTRVALESALQQQAQAAEKKGLPAEPAPAAAVARSRTRRPRRP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1041 bp	346 aa	365	1405	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIAAGRVTLRRRSLRCHGCGLTAHPLDARLGVDDFLSPHATRLACLAAASWSFDVASDRLHEFAGVPINGETIRRHSHRAAQALTRRREQEPPKAHFEDA
EGEIEFLTDGVMAPTRGGWRELKMALYLKRPLGEPAEPDAWAERELPRPTAQAAYATVADSETFAARWTPLAQALGIEPAGSLTVLGDGAEWIWNAASTQ
FPAVTQLLDVFHACEHLASAGRALFGDEAADWVERGRHSLLADGWPGLLDHVGATPVAGRTTAGQAGLDELIGDFAKHTDRLGYYGRLRTGRSIGSGGVE
SLARRMGRRLKVPGRGWCVDHLEGMASLIATADTPEWESLWTRTAA

Blast result :

Comments

ISSiac5 is 48% aa (ORFB : the transposase) similar to ISGob7.
The first ORF is a passenger gene annotated as hypothetical protein.

References

1] ISfinder annotation (2013)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Chertkov,O., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindall,B., Pomrenke,H., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2012) Direct submission GenBank.